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Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible?

Interferents influence analytical results of colorimetric methods. This work evaluated the level of analytical influence by metallic ions (K+, Ca2+ and Fe3+) on reducing sugars analysis. The difference on the reducing sugars (RS) content was analyzed by the 3,5-DNS, Somogyi-Nelson, Lane-Eynon and io...

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Principais autores: Ogando, Felipe Iwagaki Braga, Santos, Carlos Bernardo, Nouer, Pedro Aggio, Alves, Marcelo Eduardo, Aguiar, Claudio Lima
Formato: Artigo
Idioma: Português
Publicado em: Universidade Tecnológica Federal do Paraná (UTFPR) 2022
Acesso em linha: http://periodicos.utfpr.edu.br/rbta/article/view/15265
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spelling peri-article-152652023-01-16T17:50:51Z Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible? Ogando, Felipe Iwagaki Braga Santos, Carlos Bernardo Nouer, Pedro Aggio Alves, Marcelo Eduardo Aguiar, Claudio Lima Ciência e Tecnologia de Alimentos Colorimetric methods; Cane juice analysis; Fructose; Glucose; Ion chromatography Interferents influence analytical results of colorimetric methods. This work evaluated the level of analytical influence by metallic ions (K+, Ca2+ and Fe3+) on reducing sugars analysis. The difference on the reducing sugars (RS) content was analyzed by the 3,5-DNS, Somogyi-Nelson, Lane-Eynon and ion chromatography (IC) methods. The RS contents were compared to each other, using sugarcane juice samples. The same sample presented, generally, Somogyi-Nelson with higher contents, while 3,5-DNS lower. Therefore, mixed solutions with 1.5 g L-1 (proportion 1:1, v/v) of glucose and fructose were prepared with increasing doses of K+, Ca2+and Fe3+ ions (2.6; 5.2; 7.8; 10.4; 13.0 mmolc L-1). K+ underestimated the results of 3,5-DNS. Nevertheless, Ca2+ overestimated the 3,5-DNS and Lane-Eynon results. While, Fe3+ overestimated the RS content in 3,5-DNS and underestimated in Somogyi-Nelson. Regardless of the metallic ion added, ion chromatography was more stable and showed the most accurate and exact methodology. Lastly, the use of colorimetric methods can interfere with sugarcane process monitoring, especially fermentable yield. Universidade Tecnológica Federal do Paraná (UTFPR) 2022-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://periodicos.utfpr.edu.br/rbta/article/view/15265 10.3895/rbta.v16n2.15265 Revista Brasileira de Tecnologia Agroindustrial; v. 16, n. 2 (2022) 1981-3686 10.3895/rbta.v16n2 por http://periodicos.utfpr.edu.br/rbta/article/view/15265/9379 Direitos autorais 2022 CC-BY http://creativecommons.org/licenses/by/4.0
institution Universidade Tecnológica Federal do Paraná
collection PERI
language Português
format Artigo
author Ogando, Felipe Iwagaki Braga
Santos, Carlos Bernardo
Nouer, Pedro Aggio
Alves, Marcelo Eduardo
Aguiar, Claudio Lima
spellingShingle Ogando, Felipe Iwagaki Braga
Santos, Carlos Bernardo
Nouer, Pedro Aggio
Alves, Marcelo Eduardo
Aguiar, Claudio Lima
Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible?
author_sort Ogando, Felipe Iwagaki Braga
title Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible?
title_short Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible?
title_full Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible?
title_fullStr Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible?
title_full_unstemmed Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible?
title_sort control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: is it always possible?
description Interferents influence analytical results of colorimetric methods. This work evaluated the level of analytical influence by metallic ions (K+, Ca2+ and Fe3+) on reducing sugars analysis. The difference on the reducing sugars (RS) content was analyzed by the 3,5-DNS, Somogyi-Nelson, Lane-Eynon and ion chromatography (IC) methods. The RS contents were compared to each other, using sugarcane juice samples. The same sample presented, generally, Somogyi-Nelson with higher contents, while 3,5-DNS lower. Therefore, mixed solutions with 1.5 g L-1 (proportion 1:1, v/v) of glucose and fructose were prepared with increasing doses of K+, Ca2+and Fe3+ ions (2.6; 5.2; 7.8; 10.4; 13.0 mmolc L-1). K+ underestimated the results of 3,5-DNS. Nevertheless, Ca2+ overestimated the 3,5-DNS and Lane-Eynon results. While, Fe3+ overestimated the RS content in 3,5-DNS and underestimated in Somogyi-Nelson. Regardless of the metallic ion added, ion chromatography was more stable and showed the most accurate and exact methodology. Lastly, the use of colorimetric methods can interfere with sugarcane process monitoring, especially fermentable yield.
publisher Universidade Tecnológica Federal do Paraná (UTFPR)
publishDate 2022
url http://periodicos.utfpr.edu.br/rbta/article/view/15265
_version_ 1805449231539896320
score 10,814766

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