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Evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis

The objective of this work was to quantify the total content of phenolic compounds and the antioxidant activity of ethanolic extracts of acerola, guabiroba and ora-pro-nobis leaves. The analysis was performed by the DPPH free-radical method, aiming to find plants with potential use as natural antiox...

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Principais autores: Vieira, Denise Alves, Santos, Pauline Sambugaro, Haminiuk, Charles Windson Isidoro, Plata-Oviedo, Manuel Salvador Vicente
Formato: Artigo
Idioma: Português
Publicado em: Universidade Tecnológica Federal do Paraná (UTFPR) 2010
Acesso em linha: http://periodicos.utfpr.edu.br/rebrapa/article/view/3335
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spelling peri-article-33352015-11-17T19:14:38Z Evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis Vieira, Denise Alves Santos, Pauline Sambugaro Haminiuk, Charles Windson Isidoro Plata-Oviedo, Manuel Salvador Vicente Extracts from leaves; Phenolic compounds; Antioxidant activity; DPPH radical The objective of this work was to quantify the total content of phenolic compounds and the antioxidant activity of ethanolic extracts of acerola, guabiroba and ora-pro-nobis leaves. The analysis was performed by the DPPH free-radical method, aiming to find plants with potential use as natural antioxidants in foodstuff. In the extraction of phenolic compounds from leaves, acidified methanol 80% showed superior performance when compared to ethanol 80%. It was found the amounts of 1656.66, 5196.66 and 1693.33 mg GAE (gallic acid equivalent)/100 g for acerola, guabiroba and ora-pro-nobis leaves, respectively. Ethanolic extracts of guabiroba and ora-pro-nobis leaves (8.57 µg GAE/mL) presented higher antioxidant activity (92.6 and 92.76%, respectively) than BHT (10.13%), ascorbic acid (19.25%) and similar to trolox (92.79%). The extract of ora-pro-nobis presented the lowest IC50 (3.22 μg GAE/mL) showing a good ability to capture the DPPH radical. DOI: http://dx.doi.org/10.14685/rebrapa.v1i2.29 Universidade Tecnológica Federal do Paraná (UTFPR) 2010-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://periodicos.utfpr.edu.br/rebrapa/article/view/3335 10.14685/rebrapa.v1i2.29 Brazilian Journal of Food Research; v. 1, n. 2 (2010); p. 129-134 Brazilian Journal of Food Research; v. 1, n. 2 (2010); p. 129-134 2448-3184 10.14685/rebrapa.v1i2 por http://periodicos.utfpr.edu.br/rebrapa/article/view/3335/2262
institution Universidade Tecnológica Federal do Paraná
collection PERI
language Português
format Artigo
author Vieira, Denise Alves
Santos, Pauline Sambugaro
Haminiuk, Charles Windson Isidoro
Plata-Oviedo, Manuel Salvador Vicente
spellingShingle Vieira, Denise Alves
Santos, Pauline Sambugaro
Haminiuk, Charles Windson Isidoro
Plata-Oviedo, Manuel Salvador Vicente
Evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis
author_sort Vieira, Denise Alves
title Evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis
title_short Evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis
title_full Evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis
title_fullStr Evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis
title_full_unstemmed Evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis
title_sort evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis
description The objective of this work was to quantify the total content of phenolic compounds and the antioxidant activity of ethanolic extracts of acerola, guabiroba and ora-pro-nobis leaves. The analysis was performed by the DPPH free-radical method, aiming to find plants with potential use as natural antioxidants in foodstuff. In the extraction of phenolic compounds from leaves, acidified methanol 80% showed superior performance when compared to ethanol 80%. It was found the amounts of 1656.66, 5196.66 and 1693.33 mg GAE (gallic acid equivalent)/100 g for acerola, guabiroba and ora-pro-nobis leaves, respectively. Ethanolic extracts of guabiroba and ora-pro-nobis leaves (8.57 µg GAE/mL) presented higher antioxidant activity (92.6 and 92.76%, respectively) than BHT (10.13%), ascorbic acid (19.25%) and similar to trolox (92.79%). The extract of ora-pro-nobis presented the lowest IC50 (3.22 μg GAE/mL) showing a good ability to capture the DPPH radical. DOI: http://dx.doi.org/10.14685/rebrapa.v1i2.29
publisher Universidade Tecnológica Federal do Paraná (UTFPR)
publishDate 2010
url http://periodicos.utfpr.edu.br/rebrapa/article/view/3335
_version_ 1805294398965022720
score 10,814766

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