Application of an Ultra-Weak Bioluminescence Measurement System for Escherichia coli Detection in Sanitary Control
Detection and measurement of ultra-weak photon emission (UWPE) from biological samples is a promising tool with potential use in several fields such as agriculture, environmental science, food science and biomedicine. A measurement system especially designed to detect UWPE, and an application in san...
| Principais autores: | Cordeiro, Ana Carla, Marchi, Fernando, Gamba, Humberto Remigio, Fabris, Jose Luiz, Couto, Gustavo Henrique, Kalinowski, Hypolito Jose, Bertogna, Eduardo Giometti |
|---|---|
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Universidade Tecnológica Federal do Paraná (UTFPR)
2017
|
| Acesso em linha: |
http://periodicos.utfpr.edu.br/bjic/article/view/5928 |
| Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|
| id |
peri-article-5928 |
|---|---|
| recordtype |
ojs |
| spelling |
peri-article-59282022-10-19T17:34:38Z Application of an Ultra-Weak Bioluminescence Measurement System for Escherichia coli Detection in Sanitary Control Cordeiro, Ana Carla Marchi, Fernando Gamba, Humberto Remigio Fabris, Jose Luiz Couto, Gustavo Henrique Kalinowski, Hypolito Jose Bertogna, Eduardo Giometti 3.04.06.01-3; 1.05.02.02-5; 3.07.04.05-7 Ultra-Weak Bioluminescence; Escherichia coli; Sanitary Control Detection and measurement of ultra-weak photon emission (UWPE) from biological samples is a promising tool with potential use in several fields such as agriculture, environmental science, food science and biomedicine. A measurement system especially designed to detect UWPE, and an application in sanitary control of natural water resources is presented here. The system was implemented based on a dark-chamber with a photomultiplier module (PMT) cooled with a microprocessor controlled thermoelectric device coupled. The PMT detects the UWPE from the biological sample under measurement. The performance evaluation of the measuring system in terms of dark-noise and bacteria detection was performed in order to assure that it is able to realize UWPE measurements for the proposed application. The samples under test were comprised of a series of 3 control cultures of standard Escherichia coli strain, used as control, and other 3 water samples collected from a river close to a metropolitan area in Brazil. The comparison between the control and test samples has shown that the proposed application is feasible for Escherichia coli detection tests in water samples from natural water resources to assure the evaluation of their microbiologic quality. Universidade Tecnológica Federal do Paraná (UTFPR) CNPq CAPES FINEP Fundação Araucária 2017-08-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://periodicos.utfpr.edu.br/bjic/article/view/5928 10.3895/jaic.v5n1.5928 Journal of Applied Instrumentation and Control; v. 5, n. 1 (2017); 28-33 Journal of Applied Instrumentation and Control; v. 5, n. 1 (2017); 28-33 2594-3553 10.3895/ijca.v5n1 eng http://periodicos.utfpr.edu.br/bjic/article/view/5928/4360 Direitos autorais 2017 CC-BY http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidade Tecnológica Federal do Paraná |
| collection |
PERI |
| language |
Inglês |
| format |
Artigo |
| author |
Cordeiro, Ana Carla Marchi, Fernando Gamba, Humberto Remigio Fabris, Jose Luiz Couto, Gustavo Henrique Kalinowski, Hypolito Jose Bertogna, Eduardo Giometti |
| spellingShingle |
Cordeiro, Ana Carla Marchi, Fernando Gamba, Humberto Remigio Fabris, Jose Luiz Couto, Gustavo Henrique Kalinowski, Hypolito Jose Bertogna, Eduardo Giometti Application of an Ultra-Weak Bioluminescence Measurement System for Escherichia coli Detection in Sanitary Control |
| author_sort |
Cordeiro, Ana Carla |
| title |
Application of an Ultra-Weak Bioluminescence Measurement System for Escherichia coli Detection in Sanitary Control |
| title_short |
Application of an Ultra-Weak Bioluminescence Measurement System for Escherichia coli Detection in Sanitary Control |
| title_full |
Application of an Ultra-Weak Bioluminescence Measurement System for Escherichia coli Detection in Sanitary Control |
| title_fullStr |
Application of an Ultra-Weak Bioluminescence Measurement System for Escherichia coli Detection in Sanitary Control |
| title_full_unstemmed |
Application of an Ultra-Weak Bioluminescence Measurement System for Escherichia coli Detection in Sanitary Control |
| title_sort |
application of an ultra-weak bioluminescence measurement system for escherichia coli detection in sanitary control |
| description |
Detection and measurement of ultra-weak photon emission (UWPE) from biological samples is a promising tool with potential use in several fields such as agriculture, environmental science, food science and biomedicine. A measurement system especially designed to detect UWPE, and an application in sanitary control of natural water resources is presented here. The system was implemented based on a dark-chamber with a photomultiplier module (PMT) cooled with a microprocessor controlled thermoelectric device coupled. The PMT detects the UWPE from the biological sample under measurement. The performance evaluation of the measuring system in terms of dark-noise and bacteria detection was performed in order to assure that it is able to realize UWPE measurements for the proposed application. The samples under test were comprised of a series of 3 control cultures of standard Escherichia coli strain, used as control, and other 3 water samples collected from a river close to a metropolitan area in Brazil. The comparison between the control and test samples has shown that the proposed application is feasible for Escherichia coli detection tests in water samples from natural water resources to assure the evaluation of their microbiologic quality. |
| publisher |
Universidade Tecnológica Federal do Paraná (UTFPR) |
| publishDate |
2017 |
| url |
http://periodicos.utfpr.edu.br/bjic/article/view/5928 |
| _version_ |
1760450520684691456 |
| score |
10,966579 |