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A handy-assay procedure to measure simultaneously the polyphenol content and the antioxidant capacity in teas using the Folin Ciocalteu reagent

Folin Ciocalteu reagent (FCR) is commonly used for the quantification of total polyphenol content (TPC) in plants and theirs derived materials. Tea is one of the most consumed beverages in the world and widely used in developing countries in folk medicine. Tea contains several compounds (especially...

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Principais autores: Marcelino, Cícera Pimenta, Oliveira, Lucas Belini, Santana, Waila Evelyn Lima, Moya, Horacio Dorigan
Formato: Artigo
Idioma: Inglês
Publicado em: Universidade Tecnológica Federal do Paraná (UTFPR) 2020
Acesso em linha: http://periodicos.utfpr.edu.br/rebrapa/article/view/8378
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spelling peri-article-83782022-10-19T18:58:31Z A handy-assay procedure to measure simultaneously the polyphenol content and the antioxidant capacity in teas using the Folin Ciocalteu reagent Marcelino, Cícera Pimenta Oliveira, Lucas Belini Santana, Waila Evelyn Lima Moya, Horacio Dorigan Área: Química. Sub-área: Química Analítica spectrophotometry; Folin Ciocalteu reagent; antioxidant capacity; ascorbic acid equivalents; tea bags. Folin Ciocalteu reagent (FCR) is commonly used for the quantification of total polyphenol content (TPC) in plants and theirs derived materials. Tea is one of the most consumed beverages in the world and widely used in developing countries in folk medicine. Tea contains several compounds (especially polyphenols) which are believed to have antioxidant capacity. In the present study, a handy-assay procedure to measure simultaneously the TPC and the antioxidant capacity in teas using the FCR was developed. A spectrophotometric procedure using the FCR was undertaken to evaluate simultaneously the TPC and the antioxidant capacity, expressed in Trolox equivalents antioxidant capacity (TEAC), of 32 samples of 7 different types of teas. For comparison purposes, the antioxidant activity of the same samples was determined using the CUPRAC (cupric reducing antioxidant capacity) reagent. Finally, the antioxidant capacity in a cup of tea (200 mL) obtained with the FCR and expressed in ascorbic acid equivalents (AAEC) was performed. The TEAC values obtained with FCR presented a good positive correlation with the CUPRAC method (r2 = 0.853), suggesting that both reagents can be used to quantify the antioxidant capacity. The TEAC and CUPRAC values also showed good agreement with the TPC (r2 = 0.969 and r2 = 0.809, respectively) indicating that the antioxidant capacity should be due to the presence of polyphenols. The results obtained and the calculation strategy used may be an easier way to present the antioxidant capacity values to the final consumer who is most commonly unfamiliar with this important concept.  Universidade Tecnológica Federal do Paraná (UTFPR) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) 2020-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://periodicos.utfpr.edu.br/rebrapa/article/view/8378 10.3895/rebrapa.v11n3.8378 Brazilian Journal of Food Research; v. 11, n. 3 (2020); 54-70 Brazilian Journal of Food Research; v. 11, n. 3 (2020); 54-70 2448-3184 10.3895/rebrapa.v11n3 eng http://periodicos.utfpr.edu.br/rebrapa/article/view/8378/pdf Direitos autorais 2022 CC-BY http://creativecommons.org/licenses/by/4.0
institution Universidade Tecnológica Federal do Paraná
collection PERI
language Inglês
format Artigo
author Marcelino, Cícera Pimenta
Oliveira, Lucas Belini
Santana, Waila Evelyn Lima
Moya, Horacio Dorigan
spellingShingle Marcelino, Cícera Pimenta
Oliveira, Lucas Belini
Santana, Waila Evelyn Lima
Moya, Horacio Dorigan
A handy-assay procedure to measure simultaneously the polyphenol content and the antioxidant capacity in teas using the Folin Ciocalteu reagent
author_sort Marcelino, Cícera Pimenta
title A handy-assay procedure to measure simultaneously the polyphenol content and the antioxidant capacity in teas using the Folin Ciocalteu reagent
title_short A handy-assay procedure to measure simultaneously the polyphenol content and the antioxidant capacity in teas using the Folin Ciocalteu reagent
title_full A handy-assay procedure to measure simultaneously the polyphenol content and the antioxidant capacity in teas using the Folin Ciocalteu reagent
title_fullStr A handy-assay procedure to measure simultaneously the polyphenol content and the antioxidant capacity in teas using the Folin Ciocalteu reagent
title_full_unstemmed A handy-assay procedure to measure simultaneously the polyphenol content and the antioxidant capacity in teas using the Folin Ciocalteu reagent
title_sort handy-assay procedure to measure simultaneously the polyphenol content and the antioxidant capacity in teas using the folin ciocalteu reagent
description Folin Ciocalteu reagent (FCR) is commonly used for the quantification of total polyphenol content (TPC) in plants and theirs derived materials. Tea is one of the most consumed beverages in the world and widely used in developing countries in folk medicine. Tea contains several compounds (especially polyphenols) which are believed to have antioxidant capacity. In the present study, a handy-assay procedure to measure simultaneously the TPC and the antioxidant capacity in teas using the FCR was developed. A spectrophotometric procedure using the FCR was undertaken to evaluate simultaneously the TPC and the antioxidant capacity, expressed in Trolox equivalents antioxidant capacity (TEAC), of 32 samples of 7 different types of teas. For comparison purposes, the antioxidant activity of the same samples was determined using the CUPRAC (cupric reducing antioxidant capacity) reagent. Finally, the antioxidant capacity in a cup of tea (200 mL) obtained with the FCR and expressed in ascorbic acid equivalents (AAEC) was performed. The TEAC values obtained with FCR presented a good positive correlation with the CUPRAC method (r2 = 0.853), suggesting that both reagents can be used to quantify the antioxidant capacity. The TEAC and CUPRAC values also showed good agreement with the TPC (r2 = 0.969 and r2 = 0.809, respectively) indicating that the antioxidant capacity should be due to the presence of polyphenols. The results obtained and the calculation strategy used may be an easier way to present the antioxidant capacity values to the final consumer who is most commonly unfamiliar with this important concept. 
publisher Universidade Tecnológica Federal do Paraná (UTFPR)
publishDate 2020
url http://periodicos.utfpr.edu.br/rebrapa/article/view/8378
_version_ 1805450590131585024
score 10,814766

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