Análise histológica do mesêntero de Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposta a Ricinus communis L.
Parallel to the world's agricultural development is the increase in the appearance of pest insects, especially the false-moth caterpillar, Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), which has soybeans as the main target, influencing in the profitability of its production. A...
| Autor principal: | Lima, Yuri Renan Alves de |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
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Universidade Tecnológica Federal do Paraná
2020
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riut-1-110372020-11-13T13:05:38Z Análise histológica do mesêntero de Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposta a Ricinus communis L. Histological analysis of the mesenteric of Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposed to Ricinus communis L. Lima, Yuri Renan Alves de Freitas, Patricia Franchi de Warmling, Jheniffer Valmira Haas, Jucelaine Freitas, Patricia Franchi de Vieira, Claudinei de Freitas Histologia Lagartas Pragas - Controle biológico Histology Caterpillars Pests - Biological control CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::HISTOLOGIA Parallel to the world's agricultural development is the increase in the appearance of pest insects, especially the false-moth caterpillar, Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), which has soybeans as the main target, influencing in the profitability of its production. As a consequence, the scientific society has been searching for alternative methods to control this pest, highlighting the botanical extracts, since they have lower environmental impacts, and can act causing alterations mainly in the insect's midgut, damaging its physiology. In this way, the present work aims to analyze histologically the middle intestine (mesenteric) of C. includens exposed to hydroalcoholic extracts and purified fractions of Ricinus communis. Three caterpillars of C. includens were randomly collected in each treatment and controls, one at the end of 24h, 48h and 72h, which had been exposed to LC50 of purified fractions of R. communis. Thereafter, the caterpillars were killed and allocated in fixative solution for three hours, after which 70% alcohol was maintained. Then, they were dehydrated and underwent a historesin infiltration process, later being included in historesin and catalyst. The obtained blocks were cut in a microtome rotating manual EK MICRO 2.1 with a thickness of 3 to 4 μm. The sections were mounted on histological slides, stained with Hematoxylin - Eosin, and mounted with coverslips and balsam of Canada, analyzed and photographed under Axiophot - Zeiss optical photomicroscope for morphological study of the mesenteric. The present study showed that the middle intestine of C. includens did not present structural alterations for both the water and alcohol controls and for the purified fractions of R. communis, Hexane, Ethyl Acetate, Dichloromethane and Crude Extract evaluated in 24, 48 and 72 hours respectively, making further research necessary. Paralelo ao desenvolvimento agrícola mundial percebe-se o aumento de aparecimento de insetospraga, destacando-se dentre esses a lagarta falsa-medideira, Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), que tem a soja como principal alvo, influenciando pontualmente na rentabilidade de sua produção. Como consequência, a sociedade científica vem buscando métodos alternativos para o controle desta praga, destacando-se os extratos botânicos, visto que apresentam menores impactos ambientais, e podem atuar causando alterações principalmente no intestino médio (mesêntero) do inseto, danificando sua fisiologia. Dessa maneira, o presente trabalho tem por objetivo analisar histologicamente o intestino médio (mesêntero) de C. includens exposta a extratos hidroalcoólicos e frações purificadas de Ricinus communis. Foram coletadas três lagartas de 2° ínstar de C. includens, aleatoriamente em cada tratamento e testemunhas, sendo uma ao final de 24h, 48h e 72h, as quais haviam sido expostas a CL50 de frações purificadas de R. communis. A partir dai as lagartas foram mortas e alocadas em solução fixadora por três horas, sendo após, mantidas em álcool 70%. Seguidamente, estas foram desidratadas e passaram por um processo de infiltração em historesina, posteriormente sendo inclusas em historesina e catalizador. Os blocos obtidos foram cortados em micrótomo rotativo manual EK MICRO 2.1 com espessura de 3 a 4 µm. Os cortes foram montados em lâminas histológicas, corados em Hematoxilina-Eosina, e montados com lamínula e Bálsamo do Canadá, analisados e fotografados sob fotomicroscópio óptico Axiophot – Zeiss para estudo morfológico do mesêntero. O presente estudo mostrou que o intestino médio de (mesêntero) de C. includens não apresentou alterações estruturais tanto para as testemunhas água e álcool quanto para as frações purificadas de R. communis, Hexano, Acetato de Etila, Diclorometano e Extrato Bruto avaliados em 24, 48 e 72 horas respectivamente, fazendo-se necessário novos estudos investigativos. 2020-11-13T13:05:37Z 2020-11-13T13:05:37Z 2019-07-09 bachelorThesis LIMA, Yuri Renan Alves de. Análise histológica do mesêntero de Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposta a Ricinus communis L. 2019. 31 f. Trabalho de Conclusão de Curso (Graduação) - Universidade Tecnológica Federal do Paraná, Dois Vizinhos, 2019. http://repositorio.utfpr.edu.br/jspui/handle/1/11037 por openAccess application/pdf Universidade Tecnológica Federal do Paraná Dois Vizinhos Brasil Licenciatura em Ciências Biológicas UTFPR |
| institution |
Universidade Tecnológica Federal do Paraná |
| collection |
RIUT |
| language |
Português |
| topic |
Histologia Lagartas Pragas - Controle biológico Histology Caterpillars Pests - Biological control CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::HISTOLOGIA |
| spellingShingle |
Histologia Lagartas Pragas - Controle biológico Histology Caterpillars Pests - Biological control CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::HISTOLOGIA Lima, Yuri Renan Alves de Análise histológica do mesêntero de Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposta a Ricinus communis L. |
| description |
Parallel to the world's agricultural development is the increase in the appearance of pest insects, especially the false-moth caterpillar, Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), which has soybeans as the main target, influencing in the profitability of its production. As a consequence, the scientific society has been searching for alternative methods to control this pest, highlighting the botanical extracts, since they have lower environmental impacts, and can act causing alterations mainly in the insect's midgut, damaging its physiology. In this way, the present work aims to analyze histologically the middle intestine (mesenteric) of C. includens exposed to hydroalcoholic extracts and purified fractions of Ricinus communis. Three caterpillars of C. includens were randomly collected in each treatment and controls, one at the end of 24h, 48h and 72h, which had been exposed to LC50 of purified fractions of R. communis. Thereafter, the caterpillars were killed and allocated in fixative solution for three hours, after which 70% alcohol was maintained. Then, they were dehydrated and underwent a historesin infiltration process, later being included in historesin and catalyst. The obtained blocks were cut in a microtome rotating manual EK MICRO 2.1 with a thickness of 3 to 4 μm. The sections were mounted on histological slides, stained with Hematoxylin - Eosin, and mounted with coverslips and balsam of Canada, analyzed and photographed under Axiophot - Zeiss optical photomicroscope for morphological study of the mesenteric. The present study showed that the middle intestine of C. includens did not present structural alterations for both the water and alcohol controls and for the purified fractions of R. communis, Hexane, Ethyl Acetate, Dichloromethane and Crude Extract evaluated in 24, 48 and 72 hours respectively, making further research necessary. |
| format |
Trabalho de Conclusão de Curso (Graduação) |
| author |
Lima, Yuri Renan Alves de |
| author_sort |
Lima, Yuri Renan Alves de |
| title |
Análise histológica do mesêntero de Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposta a Ricinus communis L. |
| title_short |
Análise histológica do mesêntero de Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposta a Ricinus communis L. |
| title_full |
Análise histológica do mesêntero de Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposta a Ricinus communis L. |
| title_fullStr |
Análise histológica do mesêntero de Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposta a Ricinus communis L. |
| title_full_unstemmed |
Análise histológica do mesêntero de Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposta a Ricinus communis L. |
| title_sort |
análise histológica do mesêntero de chrysodeixis includens (walker, 1858) (lepidoptera: noctuidae), exposta a ricinus communis l. |
| publisher |
Universidade Tecnológica Federal do Paraná |
| publishDate |
2020 |
| citation |
LIMA, Yuri Renan Alves de. Análise histológica do mesêntero de Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), exposta a Ricinus communis L. 2019. 31 f. Trabalho de Conclusão de Curso (Graduação) - Universidade Tecnológica Federal do Paraná, Dois Vizinhos, 2019. |
| url |
http://repositorio.utfpr.edu.br/jspui/handle/1/11037 |
| _version_ |
1805313262907031552 |
| score |
10,814766 |