Sensibilidade de bactérias isoladas de fluido de corte a biocida sintético e natural
Metallurgy is a secondary sector with an important role in the country’s Gross Domestic Product, creating many jobs. In this one, machining is inserted, with an estimated expenditure of 17% in waste management, being cutting fluid part of this. Cutting fluid is a highly polluting substance with an e...
| Autor principal: | Pinto, Ana Alícia de Sá |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2020
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/12009 |
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| Resumo: |
Metallurgy is a secondary sector with an important role in the country’s Gross Domestic Product, creating many jobs. In this one, machining is inserted, with an estimated expenditure of 17% in waste management, being cutting fluid part of this. Cutting fluid is a highly polluting substance with an expensive treatment, which that’s why it is necessary to extend its use to the maximum. One of the responsible agent of the degradation of the cutting fluid, which reduces its lifetime, is bacteria. To control these microorganisms, synthetic biocides are used in the machining tanks filled with cutting fluid. These biocides can rise the polluting potential of the produced effluent, as well as being harmful to the machine operators’ health. A sustainable alternative to control the bacteria would be the substitution of the synthetic biocides by natural substances with antimicrobial characteristics, like some essential oils. This way, the objective of this work was to test the efficiency of essential oils of clove basil and clove in the inhibition of bacteria growth in the cutting fluid of mineral and vegetable origin. It was selected from the 8 colonies of bacteria that was isolated from the mineral cutting fluid and 8 from the vegetable cutting fluid of milling machine, which was in operation for 3 months with each one of the fluids. These were characterized morphotintorial and submitted to disk diffusion testing with essential oils of clove basil and clove dissolved in ethanol 0,1, 0,5 , 1,0 , 2,5 and 5% and 1% synthetic biocide. From the 8 bacterias isolated of mineral cutting fluid, 6 had coccus form, being 4 Gram positive and 2 Gram negative, the others were bacillus (1 negative and 1 positive). The fusion disk test for showed that, 7 were inhibited with clove basil essential oil, 6 with clove essential oil and 6 with biocide. Now, regarding to inhibition time, while 4 from 8 bacteria were inhibited in 15 seconds using the clove essential oil, the shorter inhibition time of bacteria using clove basil and clove essential oil was of 2 minutes. Among the isolated ones of vegetal cutting fluid, 6 were Gram positive and 2 Gram negative coccus. Among these isolates, 6 were inhibited by clove oil (1%), 4 by clove basil oil (1%) e 2 by biocide. In relation to the inhibition time of clove essential oil: 3 isolates weren’t inhibited, 2 were inhibited in 1,5 minutes, 1 in 4,5, 1 in 5 minutes and the other one in 20 minutes. Using clove basil, 5 weren’t inhibited, 1 was inhibited in 10 minutes and 2 in 20 minutes. The biocide didn’t inhibit 5 of the isolates in the analysed time, the other 3 were inhibited in 10, 20 and 30 minutes. The essential oils presents a better performance than the synthetic biocide, when compared to the same concentration, both in the number of bacteria isolates inhibited (both fluids), as in relation with the time of when this happened. |
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