Registro fonte: Controle de bactérias presentes em fluido de corte utilizando biocidas naturais

Controle de bactérias presentes em fluido de corte utilizando biocidas naturais

Cutting fluids are used to aid in the machining process, with the purpose of cooling, besides promoting lubrication, reducing friction in relation to the machined part and the cutting tool, thus increasing the efficiency of the work of the factories.In the machining system, the growth of microorgani...

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Autor principal:	Sá, Pâmela Nunes
Formato:	Dissertação
Idioma:	Português
Publicado em:	Universidade Tecnológica Federal do Paraná 2017
Assuntos:	Usinagem Contaminação microbiana Fluidos em metaloplastia Machining Microbial contamination Metal-working lubricants CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA Engenharia Sanitária
Acesso em linha:	http://repositorio.utfpr.edu.br/jspui/handle/1/2269
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spelling	riut-1-22692017-08-11T21:32:37Z Controle de bactérias presentes em fluido de corte utilizando biocidas naturais Control of bacteria present in cutting fluid using natural biocides Sá, Pâmela Nunes Prates, Kátia Valéria Marques Cardoso http://lattes.cnpq.br/3263748365906046 Moreira, Isabel Craveiro http://lattes.cnpq.br/1955070758364639 Prates, Kátia Valéria Marques Cardoso Silva Junior, Carlos Elias da Ueno, Cláudio Takeo Gonçalves, Janaína Fracaro de Souza Usinagem Contaminação microbiana Fluidos em metaloplastia Machining Microbial contamination Metal-working lubricants CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA Engenharia Sanitária Cutting fluids are used to aid in the machining process, with the purpose of cooling, besides promoting lubrication, reducing friction in relation to the machined part and the cutting tool, thus increasing the efficiency of the work of the factories.In the machining system, the growth of microorganisms can occur, since they use the cutting fluid as food source, thus reducing the useful life of the fluids and providing economic losses and causing damages to the health of the worker.To minimize the presence of microorganisms is used a chemical called biocide, which in high concentrations can cause health damage.The objective of this research was to evaluate the efficiency of the essential oils of Ocimum gratissimum (Alfavaca), Cymbopogon nardus (Citronella) Syzygium aromaticum (Clove) and Carapa guianensis (andiroba) as a control agent for the growth of bacteria in replacement of the biocide in the machining system.Initially, cutting fluid samples were collected in an automobile industry to quantify, isolate and characterize the bacteria (heterotrophic and potentially pathogenic) present in the cutting fluid.Following the isolated bacteria were tested for susceptibility to the different concentrations of the oils to define the minimum inhibitory concentration (MIC) and antibiogram to evaluate the resistance of the bacteria isolated to antibiotics.The contact time test of the essential oils and encapsulation of the essential oil of Citronella was carried out by means of the ionic gelation technique, which consisted of the simulation of reservoirs containing cut fluid contaminated with encapsulated oil, with oil mixed directly with the oil. Cutting fluid and reservoir containing only contaminated cutting fluid, in order to evaluate the best way of applying the oils in the machining system.Among the results obtained were 1.63x107 and 2.2x107 CFU / mL heterotrophic bacteria and 3.5x105 and 2x106 CFU / mL of potentially pathogenic bacteria in the first and second fluids collection, respectively. Six groups of bacteria were isolated, corresponding to the following species: Staphylococcus aureus, Enterobacter cloacae, Escherichia coli, Enterococcus faecalis, Proteus mirabilis and Acinetobacter spp.The bacterium that presented the highest resistance to the antibiotics tested was Acinetobacter spp and the ones with the highest sensitivity were Enterobacter cloacae (A2), Escherichia coli (A3) and Proteus mirabilis (A6). The oil of Syzygium aromaticum had the largest halo formed by the lowest concentration tested (0.25%) -11.07 mm, followed by Ocimum gratissimum with halo of 11 mm and Cymbopogon nardus 10.7 mm. In the test of contact time the shortest time reduction occurred in 1 minute for Ocimum gratissimum, 5 minutes for Syzygium aromaticum and 10 minutes Cymbopogon nardus for 4 of 6 bacterial isolates. The encapsulation test was performed with the Cymbopogon nardus oil, obtaining a significant reduction in the two forms of essential oil application. Fundação Araucária Fluidos de corte são utilizados para auxiliar no processo de usinagem, tendo por finalidade a refrigeração. Além disso, promove a lubrificação, a diminuição do atrito em relação à peça usinada e a ferramenta de corte, aumentando assim a eficiência do trabalho das fábricas. No sistema de usinagem, pode ocorrer o crescimento de microrganismos, uma vez que estes utilizam o fluido de corte como fonte de alimento, reduzindo assim a vida útil dos fluidos, proporcionando perdas econômicas e causando danos à saúde do operador da máquina. Para minimizar a presença de microrganismos, são utilizados produtos químicos denominados biocidas, que em altas concentrações pode causar danos à saúde. Dentro desse panorama, o objetivo desta pesquisa foi avaliar a eficiência dos óleos essenciais de Ocimum gratissimum (alfavacão), Cymbopogon winterianus (citronela) Syzygium aromaticum (cravo) e Carapa guianensis (andiroba) como agentes de controle do crescimento de bactérias em substituição ao biocida no sistema de usinagem. Inicialmente, foram realizadas coletas de fluido de corte em uma indústria automobilística para quantificar, isolar e caracterizar as bactérias (heterotróficas e potencialmente patogênicas) presentes no fluido de corte. Na sequência, as bactérias isoladas foram testadas quanto a susceptibilidade às diferentes concentrações dos óleos essenciais. O estudo procurou definir a concentração mínima inibitória (CMI) e antibiograma para avaliar a resistência a antibióticos das bactérias isoladas. Realizou-se, então, o teste de tempo de contato dos óleos essenciais e de encapsulamento do óleo essencial de citronela por meio da técnica de gelificação iônica, que consistiu em simular a situação do reservatório (i) com óleo encapsulado, (ii) com óleo misturado diretamente ao fluido de corte (iii) com fluido de corte contaminado, com o objetivo de avaliar a melhor forma de aplicação dos óleos no sistema de usinagem. Dentre os resultados, obteve-se 1,63x107 e 2,2x107 UFC/mL de bactérias heterotróficas e 3,5x105 e 2x106 UFC/mL de bactérias potencialmente patogênicas, na primeira e segunda coleta de fluidos, respectivamente. Foram isolados seis grupos de bactérias correspondentes às seguintes espécies: Staphylococcus aureus, Enterobacter cloacae, Escherichia coli, Enterococcus faecalis, Proteus mirabilis e Acinetobacter spp. A bactéria que apresentou maior resistência aos antibióticos testados foi a Acinetobacter spp As que apresentaram maior sensibilidade foram a Enterobacter cloacae (A2), Escherichia coli (A3) e Proteus mirabilis (A6). O óleo de Syzygium aromaticum teve o maior halo formado na menor concentração testada (0,25%) - 11,07 mm, seguido por Ocimum gratissimum, com halo de 11,0 mm, e Cymbopogon winterianus ,10,7 mm. No teste de tempo de contato, o menor tempo de redução de crescimento ocorreu em 1 minuto para Ocimum gratissimum; 5 minutos para Syzygium aromaticum e 10 minutos para Cymbopogon nardus. O teste de encapsulamento foi realizado com o óleo de Cymbopogon nardus, obtendo-se redução significativa nas duas formas de aplicação do óleo essencial. 2017-08-11T21:32:36Z 2017-08-11T21:32:36Z 2017-05-03 masterThesis SÁ, Pâmela Nunes. Controle de bactérias presentes em fluido de corte utilizando biocidas naturais. 2017. 83 f. Dissertação (Mestrado em Engenharia Ambiental) - Universidade Tecnológica Federal do Paraná, Londrina, 2017. http://repositorio.utfpr.edu.br/jspui/handle/1/2269 por openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Universidade Tecnológica Federal do Paraná Apucarana Londrina Brasil Programa de Pós-Graduação em Engenharia Ambiental UTFPR
institution	Universidade Tecnológica Federal do Paraná
collection	RIUT
language	Português
topic	Usinagem Contaminação microbiana Fluidos em metaloplastia Machining Microbial contamination Metal-working lubricants CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA Engenharia Sanitária
spellingShingle	Usinagem Contaminação microbiana Fluidos em metaloplastia Machining Microbial contamination Metal-working lubricants CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA Engenharia Sanitária Sá, Pâmela Nunes Controle de bactérias presentes em fluido de corte utilizando biocidas naturais
description	Cutting fluids are used to aid in the machining process, with the purpose of cooling, besides promoting lubrication, reducing friction in relation to the machined part and the cutting tool, thus increasing the efficiency of the work of the factories.In the machining system, the growth of microorganisms can occur, since they use the cutting fluid as food source, thus reducing the useful life of the fluids and providing economic losses and causing damages to the health of the worker.To minimize the presence of microorganisms is used a chemical called biocide, which in high concentrations can cause health damage.The objective of this research was to evaluate the efficiency of the essential oils of Ocimum gratissimum (Alfavaca), Cymbopogon nardus (Citronella) Syzygium aromaticum (Clove) and Carapa guianensis (andiroba) as a control agent for the growth of bacteria in replacement of the biocide in the machining system.Initially, cutting fluid samples were collected in an automobile industry to quantify, isolate and characterize the bacteria (heterotrophic and potentially pathogenic) present in the cutting fluid.Following the isolated bacteria were tested for susceptibility to the different concentrations of the oils to define the minimum inhibitory concentration (MIC) and antibiogram to evaluate the resistance of the bacteria isolated to antibiotics.The contact time test of the essential oils and encapsulation of the essential oil of Citronella was carried out by means of the ionic gelation technique, which consisted of the simulation of reservoirs containing cut fluid contaminated with encapsulated oil, with oil mixed directly with the oil. Cutting fluid and reservoir containing only contaminated cutting fluid, in order to evaluate the best way of applying the oils in the machining system.Among the results obtained were 1.63x107 and 2.2x107 CFU / mL heterotrophic bacteria and 3.5x105 and 2x106 CFU / mL of potentially pathogenic bacteria in the first and second fluids collection, respectively. Six groups of bacteria were isolated, corresponding to the following species: Staphylococcus aureus, Enterobacter cloacae, Escherichia coli, Enterococcus faecalis, Proteus mirabilis and Acinetobacter spp.The bacterium that presented the highest resistance to the antibiotics tested was Acinetobacter spp and the ones with the highest sensitivity were Enterobacter cloacae (A2), Escherichia coli (A3) and Proteus mirabilis (A6). The oil of Syzygium aromaticum had the largest halo formed by the lowest concentration tested (0.25%) -11.07 mm, followed by Ocimum gratissimum with halo of 11 mm and Cymbopogon nardus 10.7 mm. In the test of contact time the shortest time reduction occurred in 1 minute for Ocimum gratissimum, 5 minutes for Syzygium aromaticum and 10 minutes Cymbopogon nardus for 4 of 6 bacterial isolates. The encapsulation test was performed with the Cymbopogon nardus oil, obtaining a significant reduction in the two forms of essential oil application.
format	Dissertação
author	Sá, Pâmela Nunes
author_sort	Sá, Pâmela Nunes
title	Controle de bactérias presentes em fluido de corte utilizando biocidas naturais
title_short	Controle de bactérias presentes em fluido de corte utilizando biocidas naturais
title_full	Controle de bactérias presentes em fluido de corte utilizando biocidas naturais
title_fullStr	Controle de bactérias presentes em fluido de corte utilizando biocidas naturais
title_full_unstemmed	Controle de bactérias presentes em fluido de corte utilizando biocidas naturais
title_sort	controle de bactérias presentes em fluido de corte utilizando biocidas naturais
publisher	Universidade Tecnológica Federal do Paraná
publishDate	2017
citation	SÁ, Pâmela Nunes. Controle de bactérias presentes em fluido de corte utilizando biocidas naturais. 2017. 83 f. Dissertação (Mestrado em Engenharia Ambiental) - Universidade Tecnológica Federal do Paraná, Londrina, 2017.
url	http://repositorio.utfpr.edu.br/jspui/handle/1/2269
_version_	1805312761821921280
score	10,814766

Controle de bactérias presentes em fluido de corte utilizando biocidas naturais

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