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Associação de pós vegetais e Bacillus thuringiensis para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)

The most important insect pest in the global poultry production is Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), known as avian mealworm. This insect can be vector of pathogens, thus its presence in poultry houses results in several losses to the productive chain, such as reduction of...

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Autor principal: Alencar, Rafael Vido de
Formato: Dissertação
Idioma: Português
Publicado em: Universidade Tecnológica Federal do Paraná 2017
Assuntos:
Aves - Criação
Pragas - Controle
Bacillus thuringiensis
Aviculture
Pests - Control
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::PRODUCAO ANIMAL
Ciências Agrárias
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spelling riut-1-23822017-08-31T14:09:11Z Associação de pós vegetais e Bacillus thuringiensis para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) Association between Bacillus thuringiensis and vegetable powders for the control of Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) Alencar, Rafael Vido de Gouvea, Alfredo de http://lattes.cnpq.br/0808349137348123 Silva, Everton Ricardi Lozano da Potrich, Michele http://lattes.cnpq.br/0137021895397436 http://lattes.cnpq.br/6017285848848713 Gouvea, Alfredo de Mertz, Natalia Ramos Potrich, Michele Aves - Criação Pragas - Controle Bacillus thuringiensis Aviculture Pests - Control CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::PRODUCAO ANIMAL Ciências Agrárias The most important insect pest in the global poultry production is Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), known as avian mealworm. This insect can be vector of pathogens, thus its presence in poultry houses results in several losses to the productive chain, such as reduction of weight gain and damages of carcasses. The control A. diaperinus is done mainly with synthetic chemicals that are harmful to the environment, leave residues in the meat and contaminate the workers. Therefore, new alternative of controls that minimize impacts related to productivity and the environment are needed. The objective of this study was to evaluate the effect of vegetable powders, Bacillus thuringiensis and their association upon A. diaperinus larvae and adults at laboratory conditions. Three B. thuringiensis strains have been tested, ips82, br147 and br81, at concentration of 3x108 colony forming unit/ml added to poultry food. The mixture was divided in five acrylic dishes with 12 wells each and 20 larvae or adults of A. diaperinus were allocated in each well. Plants used for vegetable powders were rue, surinam cherry, mint and orange at concentration of 20%. The powders (20g) were added and mixed to 80g of sterilized feed for chickens. The mixture was divided in five parts and added to Petri dishes, where 20 larvae or adults of A. diaperinus were allocated in each replicate. The association of methods consisted in preparing suspensions of B. thuringiensis strains, br81, ips82 and br147 at the concentration of 3x108 spores/ml to the vegetable powder (5%) included in 22.8g of commercial feed for chicken. The mealworm mortality was assessed in all experiments for 10 days. Surinam cherry extract caused 63.73% accumulated mortality of the mealworm larvae, differing from the other extracts. As for mortality of adults, surinam cherry and orange presented 70% and 61.55% respectively with significant difference from the control. The three B. thuringiensis strains differed significantly from the control group for larvae cumulative mortality resulting respectively in 60%, 55% and 61.66%, whereas control group was only 15%. No significant differences were observed for adults. The association of methods presented significant larvae mortality when compared to the control, but only treatment 3 (br81 + 5% surinam cherry extract) presented over than 55% mortality. Treatment 2 (ips 82 + 5% surinam cherry extract) caused significant mortality in adults. Therefore, B. thuringiensis associated with vegetable powders at the described concentrations and associations showed insecticidal effect on larvae and adults of A. diaperinus. O inseto-praga de maior importância na produção avícola mundial é Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), conhecido como cascudinho de aviário. Sua presença nos galpões avícolas ocasiona diversos prejuízos à cadeia, sendo eles, ransmissores de patógenos, perdas no ganho de peso das aves, danificação de instalações, prejuízos nas carcaças. O controle de A. diaperinus é feito principalmente com produtos químicos sintéticos que trazem malefícios como a contaminação do ambiente, deixam resíduos nas carcaças dos animais e contaminação de aplicadores. Desta forma, é necessário encontrar novas alternativas de controle que visam minimizar impactos ligados à produtividade e ao meio ambiente. Neste sentido, o objetivo deste trabalho foi avaliar o efeito de pós vegetais e Bacillus thuringiensis e a associação destes sobre larvas e adultos de A. diaperinus, em condições de laboratório. Foram testadas três linhagens de B. thuringiensis, ips82, br147 e br81, na concentração de 3x 108, adicionados à ração para aves. A mistura foi dividida em cinco placas de acrílico com 12 poços cada, sendo alocadas 12 larvas ou 12 adultos de A. diaperinus em cada poço. As plantas utilizadas para a obtenção dos pós vegetais foram arruda, pitanga, menta e laranjeira na concentração de 20%. Os pós (20g) foram adicionados e misturados a 80 g de ração de frangos esterilizada. A mistura foi dividida em cinco partes e adicionada em placas de petri, sendo alocadas 20 larvas ou 20 adultos de A. diaperinus em cada repetição. A associação dos métodos consistiu na preparação de suspensões das linhagens br 81 ips 82 e br 147 de B. thuringiensis, na concentração 3 × 108 UFC/mL à concentração do pó vegetal (5%) de pitanga incluídos em 22,8 g de ração comercial para frangos. Todos os experimentos foram avaliados a mortalidade dos cascudinhos, durante dez dias. O extrato de pitanga ocasionou 63,73% de mortalidade acumulada em larvas de cascudinho, diferindo dos demais extratos. Já para a mortalidade em adultos, a pintanga e a laranja 70% e 61,55% respectivamente diferiram significamente da testemunha. No experimento com Bt para larvas as três subespécies diferiram significamente da testemunha, apresentando mortalidade acumulada respectivamente de 60%, 55% e 61,66%, já a testemunha apresentou apenas 15%. Para adultos as três subespécies não diferiram significamente da testemunha. A associação de métodos apresentou mortalidade para larvas, significativamente se comparado com a testemunha, porém apenas o tratamento 3 (br81+5% de extrato de pitanga) obteve acima de 55% de mortalidade. Para adultos os tratamentos 2 (ips82+5% de extrato de pitanga) e 3 (br81+5% de extrato de pitanga) ocasionaram mortalidade significativamente se comparados com a testemunha. A associação de bactéria com extrato apresentou efeito inseticida para larvas e adultos. 2017-08-31T14:09:00Z 2017-08-31T14:09:00Z 2015-09-18 masterThesis ALENCAR, Rafael Vido de. Associação de pós vegetais e Bacillus thuringiensis para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). 2015. 55 f. Dissertação (Mestrado em Zootecnia) - Universidade Tecnológica Federal do Paraná, Dois Vizinhos, 2015. http://repositorio.utfpr.edu.br/jspui/handle/1/2382 por openAccess application/pdf Universidade Tecnológica Federal do Paraná Dois Vizinhos Brasil Programa de Pós-Graduação em Zootecnia UTFPR
institution Universidade Tecnológica Federal do Paraná
collection RIUT
language Português
topic Aves - Criação
Pragas - Controle
Bacillus thuringiensis
Aviculture
Pests - Control
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::PRODUCAO ANIMAL
Ciências Agrárias
spellingShingle Aves - Criação
Pragas - Controle
Bacillus thuringiensis
Aviculture
Pests - Control
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::PRODUCAO ANIMAL
Ciências Agrárias
Alencar, Rafael Vido de
Associação de pós vegetais e Bacillus thuringiensis para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
description The most important insect pest in the global poultry production is Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), known as avian mealworm. This insect can be vector of pathogens, thus its presence in poultry houses results in several losses to the productive chain, such as reduction of weight gain and damages of carcasses. The control A. diaperinus is done mainly with synthetic chemicals that are harmful to the environment, leave residues in the meat and contaminate the workers. Therefore, new alternative of controls that minimize impacts related to productivity and the environment are needed. The objective of this study was to evaluate the effect of vegetable powders, Bacillus thuringiensis and their association upon A. diaperinus larvae and adults at laboratory conditions. Three B. thuringiensis strains have been tested, ips82, br147 and br81, at concentration of 3x108 colony forming unit/ml added to poultry food. The mixture was divided in five acrylic dishes with 12 wells each and 20 larvae or adults of A. diaperinus were allocated in each well. Plants used for vegetable powders were rue, surinam cherry, mint and orange at concentration of 20%. The powders (20g) were added and mixed to 80g of sterilized feed for chickens. The mixture was divided in five parts and added to Petri dishes, where 20 larvae or adults of A. diaperinus were allocated in each replicate. The association of methods consisted in preparing suspensions of B. thuringiensis strains, br81, ips82 and br147 at the concentration of 3x108 spores/ml to the vegetable powder (5%) included in 22.8g of commercial feed for chicken. The mealworm mortality was assessed in all experiments for 10 days. Surinam cherry extract caused 63.73% accumulated mortality of the mealworm larvae, differing from the other extracts. As for mortality of adults, surinam cherry and orange presented 70% and 61.55% respectively with significant difference from the control. The three B. thuringiensis strains differed significantly from the control group for larvae cumulative mortality resulting respectively in 60%, 55% and 61.66%, whereas control group was only 15%. No significant differences were observed for adults. The association of methods presented significant larvae mortality when compared to the control, but only treatment 3 (br81 + 5% surinam cherry extract) presented over than 55% mortality. Treatment 2 (ips 82 + 5% surinam cherry extract) caused significant mortality in adults. Therefore, B. thuringiensis associated with vegetable powders at the described concentrations and associations showed insecticidal effect on larvae and adults of A. diaperinus.
format Dissertação
author Alencar, Rafael Vido de
author_sort Alencar, Rafael Vido de
title Associação de pós vegetais e Bacillus thuringiensis para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
title_short Associação de pós vegetais e Bacillus thuringiensis para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
title_full Associação de pós vegetais e Bacillus thuringiensis para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
title_fullStr Associação de pós vegetais e Bacillus thuringiensis para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
title_full_unstemmed Associação de pós vegetais e Bacillus thuringiensis para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
title_sort associação de pós vegetais e bacillus thuringiensis para o controle de alphitobius diaperinus (panzer) (coleoptera: tenebrionidae)
publisher Universidade Tecnológica Federal do Paraná
publishDate 2017
citation ALENCAR, Rafael Vido de. Associação de pós vegetais e Bacillus thuringiensis para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). 2015. 55 f. Dissertação (Mestrado em Zootecnia) - Universidade Tecnológica Federal do Paraná, Dois Vizinhos, 2015.
url http://repositorio.utfpr.edu.br/jspui/handle/1/2382
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score 10,814766

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