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Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims

Regarding the important actions attributed to antibiotics, it is understood the necessary and constant search for solutions that allow from the control of bacterial development, to its eradication. Over the years, many studies are being developed regarding the use of vegetables as precursors of prod...

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Autor principal:	Junglaus, Douglas
Formato:	Dissertação
Idioma:	Português
Publicado em:	Universidade Tecnológica Federal do Paraná 2022
Assuntos:	Maracujá Química vegetal Escherichia coli Passion fruit Botanical chemistry CNPQ::CIENCIAS BIOLOGICAS Biotecnologia
Acesso em linha:	http://repositorio.utfpr.edu.br/jspui/handle/1/27786
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spelling	riut-1-277862022-04-05T06:08:13Z Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims Evaluation of the antimicrobial activity of aqueous and metanolic vegetable extracts from Passiflora edulis Sims Junglaus, Douglas Fagundes, Regiane Slongo https://orcid.org/0000-0002-8560-916X http://lattes.cnpq.br/3383322132577252 Lobo, Viviane da Silva https://orcid.org/0000-0001-7099-9727 http://lattes.cnpq.br/3219620885362801 Fagundes, Regiane Slongo https://orcid.org/0000-0002-8560-916X http://lattes.cnpq.br/3383322132577252 Silva, Claudia Tatiana Araujo da Cruz http://lattes.cnpq.br/8415444857568360 Dalposso, Gustavo Henrique https://orcid.org/0000-0003-2097-5748 http://lattes.cnpq.br/8040071176709565 Maracujá Química vegetal Escherichia coli Passion fruit Botanical chemistry Escherichia coli CNPQ::CIENCIAS BIOLOGICAS Biotecnologia Regarding the important actions attributed to antibiotics, it is understood the necessary and constant search for solutions that allow from the control of bacterial development, to its eradication. Over the years, many studies are being developed regarding the use of vegetables as precursors of products that allow treatment or cure of diseases, pest control, among other purposes. From this information and characteristics, this work aimed to analyze the antimicrobial activity of plant extracts, aqueous and methanolic, of the species Passiflora edulis Sims, against the bacteria Escherichia coli and Staphylococcus aureus. The collection of the plant material was collected, referring to the leaves, peels and seeds of the yellow passion fruit. Subsequently, in order to obtain the aqueous and methanolic extracts, drying, maceration, grinding, addition of solvents, filtration, rotoevaporation were carried out, according to each stage and methodology duly pre-established. The following groups were established: Group 1, Group 2, Group3 (both through extracts obtained from leaves collected in two stages), Test Group - macerated leaves, Test Group - seeds and Test Group - peels, totaling 114 samples. Statistical calculations were performed with the data obtained from each of the samples, and the variability of each group was determined, obtaining high coefficients of variation, observing greater variability in samples with concentrations of methanolic or aqueous solvent of proportions 70:30. The extracts obtained were used to evaluate the antimicrobial action by means of disk diffusion methods, which allowed sizing the inhibition halo, and broth microdilution, in which it was possible to establish the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). After the experiments and methods performed, among the yellow passion fruit plant extracts obtained, the inhibitory action was observed only in the fruit seed extract against the microorganism S. aureus. Despite the fact that few samples of the seed extract were obtained, which inhibited the growth of the microorganism, it became evident the need to develop new studies in more depth, identifying possible characteristics present in the plant extracts that may have an inhibitory function against other species bacteria. No que tange as ações importantes atribuídas aos antibióticos, entende-se a necessária e constante busca por soluções que possibilitem desde o controle do desenvolvimento bacteriano, até sua erradicação. Com o avançar dos anos, muitos estudos estão sendo desenvolvidos quanto à utilização de vegetais como precursores de produtos que possibilitem tratamento ou cura de doenças, controle de pragas, dentre outros fins. A partir destas informações e características, este trabalho visou analisar a atividade antimicrobiana dos extratos vegetais, aquoso e metanólico, da espécie Passiflora edulis Sims, frente às bactérias Escherichia coli e Staphylococcus aureus. Realizou-se a coleta do material vegetal, sendo este referente às folhas, cascas e sementes do maracujá-amarelo. Posteriormente, para a obtenção dos extratos aquoso e metanólico foram realizados os processos de secagem, maceração, trituração, adição dos solventes, filtragem, rotoevaporação, conforme cada etapa e metodologia devidamente preestabelecida. Foram estabelecidos os seguintes grupos: Grupo 1, Grupo 2, Grupo3 (ambos através de extratos obtidos por meio de folhas coletadas em duas etapas), Grupo Teste – folhas maceradas, Grupo Teste – sementes e Grupo Teste – cascas, totalizando 114 amostras. Realizou-se cálculos estatísticos com os dados obtidos de cada uma das amostras, e determinada a variabilidade de cada grupo, obtendo coeficientes de variação altos, observando variabilidade maior nas amostras com concentrações de solvente metanólico ou aquoso de proporções 70:30. Os extratos obtidos foram utilizados para avaliar a ação antimicrobiana por meio dos métodos de difusão em disco, o qual permitiu dimensionar o halo de inibição, e de microdiluição em caldo, no qual foi possível estabelecer a Concentração Inibitória Mínima (CIM) e Concentração Bactericida Mínima (CBM). Após os experimentos e métodos realizados, dentre os extratos vegetais de maracujá-amarelo obtidos, observou-se a ação inibitória apenas no extrato de sementes do fruto frente ao microrganismo S. aureus. Apesar de terem sido obtidas poucas amostragens do extrato de semente, o qual inibiu o crescimento do microrganismo, ficou evidente a necessidade do desenvolvimento de novos estudos de maneira mais aprofundada, identificando possíveis características presentes nos extratos vegetais que venham a ter a função inibitória perante outras bactérias. 2022-04-04T12:14:05Z 2022-04-04T12:14:05Z 2022-02-09 masterThesis JUNGLAUS, Douglas. Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims. 2022. Dissertação (Mestrado em Tecnologias em Biociências) - Universidade Tecnológica Federal do Paraná, Toledo, 2022. http://repositorio.utfpr.edu.br/jspui/handle/1/27786 por openAccess https://creativecommons.org/licenses/by-nc/4.0/ application/pdf Universidade Tecnológica Federal do Paraná Toledo Brasil Tecnologias em Biociências UTFPR
institution	Universidade Tecnológica Federal do Paraná
collection	RIUT
language	Português
topic	Maracujá Química vegetal Escherichia coli Passion fruit Botanical chemistry Escherichia coli CNPQ::CIENCIAS BIOLOGICAS Biotecnologia
spellingShingle	Maracujá Química vegetal Escherichia coli Passion fruit Botanical chemistry Escherichia coli CNPQ::CIENCIAS BIOLOGICAS Biotecnologia Junglaus, Douglas Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims
description	Regarding the important actions attributed to antibiotics, it is understood the necessary and constant search for solutions that allow from the control of bacterial development, to its eradication. Over the years, many studies are being developed regarding the use of vegetables as precursors of products that allow treatment or cure of diseases, pest control, among other purposes. From this information and characteristics, this work aimed to analyze the antimicrobial activity of plant extracts, aqueous and methanolic, of the species Passiflora edulis Sims, against the bacteria Escherichia coli and Staphylococcus aureus. The collection of the plant material was collected, referring to the leaves, peels and seeds of the yellow passion fruit. Subsequently, in order to obtain the aqueous and methanolic extracts, drying, maceration, grinding, addition of solvents, filtration, rotoevaporation were carried out, according to each stage and methodology duly pre-established. The following groups were established: Group 1, Group 2, Group3 (both through extracts obtained from leaves collected in two stages), Test Group - macerated leaves, Test Group - seeds and Test Group - peels, totaling 114 samples. Statistical calculations were performed with the data obtained from each of the samples, and the variability of each group was determined, obtaining high coefficients of variation, observing greater variability in samples with concentrations of methanolic or aqueous solvent of proportions 70:30. The extracts obtained were used to evaluate the antimicrobial action by means of disk diffusion methods, which allowed sizing the inhibition halo, and broth microdilution, in which it was possible to establish the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). After the experiments and methods performed, among the yellow passion fruit plant extracts obtained, the inhibitory action was observed only in the fruit seed extract against the microorganism S. aureus. Despite the fact that few samples of the seed extract were obtained, which inhibited the growth of the microorganism, it became evident the need to develop new studies in more depth, identifying possible characteristics present in the plant extracts that may have an inhibitory function against other species bacteria.
format	Dissertação
author	Junglaus, Douglas
author_sort	Junglaus, Douglas
title	Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims
title_short	Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims
title_full	Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims
title_fullStr	Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims
title_full_unstemmed	Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims
title_sort	avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de passiflora edulis sims
publisher	Universidade Tecnológica Federal do Paraná
publishDate	2022
citation	JUNGLAUS, Douglas. Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims. 2022. Dissertação (Mestrado em Tecnologias em Biociências) - Universidade Tecnológica Federal do Paraná, Toledo, 2022.
url	http://repositorio.utfpr.edu.br/jspui/handle/1/27786
_version_	1805322378154082304
score	10,814766

Avaliação da atividade antimicrobiana de extratos vegetais aquoso e metanólico de Passiflora edulis Sims

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