Toxicidade em nível celular e fitotoxidade do octocrileno em Allium cepa L.
The study aimed to evaluate the toxicity at cellular level and phytotoxicity of the ultraviolet filter octocrylene in Allium cepa L., under justification of practicality and recognition of this test organism in trials of the kind, as well as the potential threat of emerging pollutants, increased sal...
| Autor principal: | Santo, Diego Espirito |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2022
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/29378 |
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| Resumo: |
The study aimed to evaluate the toxicity at cellular level and phytotoxicity of the ultraviolet filter octocrylene in Allium cepa L., under justification of practicality and recognition of this test organism in trials of the kind, as well as the potential threat of emerging pollutants, increased sales of products with indicative of sun protection and governmental retrospect to the environment in the country. The experiment was conducted at the Universidade Tecnológica Federal do Paraná (UTFPR) in Campo Mourão, between October and December 2021, starting with the dilution of the compound in concentrations of 100 and 200 µg/L in DMSO, and 300 µg/L in DMSO and Tween® 20. Germination and root growth were estimated after 8 days of the plated seeds in an incubator, and cytotoxicity and genotoxicity effects were obtained in bulb meristems at contact times of 24 and 48h, all in quintuplicate. Despite not being cytotoxic or genotoxic under these conditions, octocrylene induced an increase in the number of dividing cells and, consequently, in the mitotic index, which was attributed to hormesis, a dose-response phenomenon where the organism is stimulated and has a performance gain by fighting the stressor at low levels of exposure. On the other hand, there were decreases in the number of germinated seeds and in root length, which seems to be a model of delayed mitosis caused by overproduction of reactive oxygen species, which may have interfered with parameters such as chromosome movement and nuclear envelope dynamics, causing an accumulation of cells in prophase and preventing the continuity of the cycle. A single exception, when prophase accumulation did not prevent roots from growing, may have been indicative of cell elongation in the basal section. The study provided toxicologically relevant data in A. cepa and fills a scientific gap of risk estimates focused exclusively on aquatic animals and at concentrations much higher than those recorded in real matrices. It is hoped that it will help build a robust database to drive investments in monitoring and removal technologies, legislative calls for restrictions and development of new ecologically viable filters, and to inform society of the dangers around them. |
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