Potencial bactericida de biocarvões do pseudocaule da bananeira (Musa sp.)
The access to quality drinking water is a basic need that represents as a major problem for many people around the world, about 2.1 billion people in 2017 according to the World Health Organization. One of the critical factors for the standards of water quality for human consumption is the control o...
| Autor principal: | Barbosa, Eduardo Ghiraldi |
|---|---|
| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
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Universidade Tecnológica Federal do Paraná
2023
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riut-1-306532023-03-01T06:07:33Z Potencial bactericida de biocarvões do pseudocaule da bananeira (Musa sp.) Bactericidal potencial of banana pseudostem biochars (Musa sp.) Barbosa, Eduardo Ghiraldi Scheufele, Fabiano Bisinella Busso, Cleverson Scheufele, Fabiano Bisinella Maniglia, Thiago Cintra Jacomini, Débora Carbono ativado Água - Purificação Filtros e filtração Carbon, Activated Water - Purification Filters and filtration CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA::TRATAMENTO DE AGUAS DE ABASTECIMENTO E RESIDUARIAS::TECNICAS AVANCADAS DE TRATAMENTO DE AGUAS The access to quality drinking water is a basic need that represents as a major problem for many people around the world, about 2.1 billion people in 2017 according to the World Health Organization. One of the critical factors for the standards of water quality for human consumption is the control of the concentration of microorganisms present in it, since these living beings cause not only changes in taste and odor, but also pipes and equipment corrosion, contaminate food and spread diseases among human beings. Based on the problems generated by the contamination of microorganisms, mainly in water sources, this work seeks to evaluate the bactericidal potential of activated carbon obtained from the banana pseudostem (CA-PCB in portuguese), to investigate its possible use in filters or other equipment that act on bacterial inhibition. To determine the charcoal inhibition capacities, experiments were carried out with strains of E. coli and P. aeruginosa, comparing different production conditions of CA-PCB by a Box-Behnken (BB) design, with variations in the concentrations of the activating agent ZnCl2 (Cimp), heating rate (R) and activation temperature (Tativ) in the pyrolysis and activation process. In addition, other activated carbons (obtained from açaí and a commercial one impregnated with Ag/Cu) were also evaluated for comparison purposes. Thus, as an indicator of bactericidal potential, the formation of inhibitory halos by coals in Petri dishes expressed by the areas of inhibition halos was evaluated as a variable response of the BB design, for each of the studied bacterial strains. In addition, the stability of the coal was evaluated (by hot washing and by cold running water), the pH of the CA-PCB was determined, as well as the possibility of leaching in terms of pH variations due to contact with carbons, as well as the leaching of elements into the aqueous phase. The coals were also characterized as to their morphology (by microscopy) as well as their elemental composition. In general, different behaviors were observed in the formation of inhibitory halos for each strain, while in plaques with P. aeruginosa the coals with the highest concentrations of impregnating material (Cimp = 2 mol L-1) had the highest bactericidal potential (halo areas above 3 cm2), with E. coli the best results were obtained for coals with production variables at more central values (Cimp = 1 mol L-1; R = 10 °C min-1; Tativ = 600 °C). Additionally, a partial loss of the bactericidal potential of CA-PCB was observed after the leaching experiment (cold wash with running water) and a total loss of inhibitory capacity after the pH determination procedure (hot wash), for strain P. aeruginosa. The pH of the CA-PCB was 4.06 and, therefore, aiming to investigate the inhibitory mechanisms, it was found that the acidic pH (< 4) can be characterized as the cause of inhibition for the CA-PCB, however this factor was not confirmed (at least not as the only cause of the bactericidal capacity). In addition, the experiment to evaluate the possible leaching of elements from CA-PCB indicated an irrelevant release of zinc (Zn) and a remarkable removal of chlorine (>97%) by activated carbon, which is a promising characteristic of this carbon (based on the NBR 10698 – which is a Brazilian regulatory standard that states about the quality standards of water filters). Based on the obtained results, it can be concluded that the use of CA-PCB is very promising for the manufacture of filters, for the removal of organic and inorganic compounds, and also with bactericidal potential. However, further studies are still necessary for a comprehensive understanding of the stability behavior of this activated carbon, in addition to identifying the inhibitory mechanisms provided by the material that promotes the bactericidal activity. O acesso à água potável de qualidade é uma necessidade básica que afeta diversas pessoas ao redor do mundo, cerca de 2,1 bilhões de pessoas em 2017 segundo a OMS. Um dos fatores decisivos que caracterizam a água de qualidade para consumo humano é o controle da concentração de microrganismos, visto que estes seres vivos causam não apenas alterações no sabor e odor, mas também contaminam alimentos e disseminam doenças dentre os seres humanos. Baseando-se nos problemas gerados por contaminações de microrganismos na água, este trabalho busca avaliar o potencial bactericida do carvão ativado obtido a partir do pseudocaule da bananeira (CA-PCB), para avaliar sua possível utilização em filtros com atuação na inibição bacteriana. Para determinar as capacidades de inibição do carvão foram desenvolvidos experimentos com cepas de Escherichia coli e Pseudomonas aeruginosa, comparando diferentes condições de produção dos CA-PCB por um planejamento Box-Behnken (BB), com variações das concentrações do agente ativante ZnCl2 (Cimp), taxa de aquecimento (R) e temperatura de ativação (Tativ) no processo de ativação. Além disso, foram avaliados em conjunto outros carvões ativados (de açaí e comercial impregnado com Ag/Cu) para fins de comparação. Desta forma, avaliou-se como indicador do potencial bactericida, a formação de halos inibitórios pelos carvões em placas de Petri, com as áreas dos halos de inibição como variável resposta do planejamento BB, para cada uma das cepas bacterianas estudadas. Além disso, foi avaliada a estabilidade do carvão (com lavagem à quente, e à temperatura ambiente com água corrente), determinou-se o pH do CA-PCB, bem como a possibilidade de lixiviação (por variações do pH devido ao contato com o carvão, bem como a lixiviação de elementos para a fase aquosa). Os carvões também foram caracterizados quanto à sua morfologia (por microscopia), e pela composição elementar. Em geral, verificou-se comportamentos diferentes na formação de halos inibitórios de cada cepa, enquanto nas placas com P. aeruginosa os carvões com maiores valores de Cimp (Cimp = 2 mol L-1) apresentaram os maiores potenciais bactericidas (APA = 3 cm2), com a E. coli os melhores resultados foram obtidos pelos carvões com as variáveis de produção em valores mais centrais (Cimp = 1 mol L-1; R = 10 °C min-1; Tativ = 600 °C). Adicionalmente, foi observada uma perda parcial do potencial bactericida do CA-PCB após o experimento de lixiviação e perda total da capacidade inibitória após o procedimento de determinação do pH, para a cepa P. aeruginosa. O pH do CA-PCB foi de 4,06 e, portanto, visando investigar os mecanismos inibitórios, verificou-se que o pH ácido (< 4) poderia se caracterizar como a causa da inibição para os CA-PCB. No entanto, esse fator não foi confirmado como a única causa do potencial bactericida. Além disso, o experimento de avaliação da lixiviação de elementos do CA-PCB, indicou uma liberação irrelevante de zinco (Zn) e uma remoção de cloro superior a 97% pelo carvão ativado, sendo está uma característica promissora deste carvão (frente à NBR 10698). Com os resultados obtidos pode-se concluir que a utilização do CA-PCB é muito promissora para a confecção de filtros para a remoção de compostos orgânicos e inorgânicos e, ainda, com potencial bactericida. No entanto, são necessários mais estudos para uma melhor compreensão do comportamento da estabilidade do carvão e entendimento dos mecanismos inibitórios do material promovendo a ação bactericida. 2023-02-28T16:36:58Z 2023-02-28T16:36:58Z 2021-08-26 bachelorThesis BARBOSA, Eduardo Ghiraldi. Potencial bactericida de biocarvões do pseudocaule da bananeira (Musa sp.). 2021. Trabalho de Conclusão de Curso (Bacharelado em Engenharia de Bioprocessos e Biotecnologia) - Universidade Tecnológica Federal do Paraná, Toledo, 2021. http://repositorio.utfpr.edu.br/jspui/handle/1/30653 por openAccess application/pdf Universidade Tecnológica Federal do Paraná Toledo Brasil Engenharia de Bioprocessos e Biotecnologia UTFPR |
| institution |
Universidade Tecnológica Federal do Paraná |
| collection |
RIUT |
| language |
Português |
| topic |
Carbono ativado Água - Purificação Filtros e filtração Carbon, Activated Water - Purification Filters and filtration CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA::TRATAMENTO DE AGUAS DE ABASTECIMENTO E RESIDUARIAS::TECNICAS AVANCADAS DE TRATAMENTO DE AGUAS |
| spellingShingle |
Carbono ativado Água - Purificação Filtros e filtração Carbon, Activated Water - Purification Filters and filtration CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA::TRATAMENTO DE AGUAS DE ABASTECIMENTO E RESIDUARIAS::TECNICAS AVANCADAS DE TRATAMENTO DE AGUAS Barbosa, Eduardo Ghiraldi Potencial bactericida de biocarvões do pseudocaule da bananeira (Musa sp.) |
| description |
The access to quality drinking water is a basic need that represents as a major problem for many people around the world, about 2.1 billion people in 2017 according to the World Health Organization. One of the critical factors for the standards of water quality for human consumption is the control of the concentration of microorganisms present in it, since these living beings cause not only changes in taste and odor, but also pipes and equipment corrosion, contaminate food and spread diseases among human beings. Based on the problems generated by the contamination of microorganisms, mainly in water sources, this work seeks to evaluate the bactericidal potential of activated carbon obtained from the banana pseudostem (CA-PCB in portuguese), to investigate its possible use in filters or other equipment that act on bacterial inhibition. To determine the charcoal inhibition capacities, experiments were carried out with strains of E. coli and P. aeruginosa, comparing different production conditions of CA-PCB by a Box-Behnken (BB) design, with variations in the concentrations of the activating agent ZnCl2 (Cimp), heating rate (R) and activation temperature (Tativ) in the pyrolysis and activation process. In addition, other activated carbons (obtained from açaí and a commercial one impregnated with Ag/Cu) were also evaluated for comparison purposes. Thus, as an indicator of bactericidal potential, the formation of inhibitory halos by coals in Petri dishes expressed by the areas of inhibition halos was evaluated as a variable response of the BB design, for each of the studied bacterial strains. In addition, the stability of the coal was evaluated (by hot washing and by cold running water), the pH of the CA-PCB was determined, as well as the possibility of leaching in terms of pH variations due to contact with carbons, as well as the leaching of elements into the aqueous phase. The coals were also characterized as to their morphology (by microscopy) as well as their elemental composition. In general, different behaviors were observed in the formation of inhibitory halos for each strain, while in plaques with P. aeruginosa the coals with the highest concentrations of impregnating material (Cimp = 2 mol L-1) had the highest bactericidal potential (halo areas above 3 cm2), with E. coli the best results were obtained for coals with production variables at more central values (Cimp = 1 mol L-1; R = 10 °C min-1; Tativ = 600 °C). Additionally, a partial loss of the bactericidal potential of CA-PCB was observed after the leaching experiment (cold wash with running water) and a total loss of inhibitory capacity after the pH determination procedure (hot wash), for strain P. aeruginosa. The pH of the CA-PCB was 4.06 and, therefore, aiming to investigate the inhibitory mechanisms, it was found that the acidic pH (< 4) can be characterized as the cause of inhibition for the CA-PCB, however this factor was not confirmed (at least not as the only cause of the bactericidal capacity). In addition, the experiment to evaluate the possible leaching of elements from CA-PCB indicated an irrelevant release of zinc (Zn) and a remarkable removal of chlorine (>97%) by activated carbon, which is a promising characteristic of this carbon (based on the NBR 10698 – which is a Brazilian regulatory standard that states about the quality standards of water filters). Based on the obtained results, it can be concluded that the use of CA-PCB is very promising for the manufacture of filters, for the removal of organic and inorganic compounds, and also with bactericidal potential. However, further studies are still necessary for a comprehensive understanding of the stability behavior of this activated carbon, in addition to identifying the inhibitory mechanisms provided by the material that promotes the bactericidal activity. |
| format |
Trabalho de Conclusão de Curso (Graduação) |
| author |
Barbosa, Eduardo Ghiraldi |
| author_sort |
Barbosa, Eduardo Ghiraldi |
| title |
Potencial bactericida de biocarvões do pseudocaule da bananeira (Musa sp.) |
| title_short |
Potencial bactericida de biocarvões do pseudocaule da bananeira (Musa sp.) |
| title_full |
Potencial bactericida de biocarvões do pseudocaule da bananeira (Musa sp.) |
| title_fullStr |
Potencial bactericida de biocarvões do pseudocaule da bananeira (Musa sp.) |
| title_full_unstemmed |
Potencial bactericida de biocarvões do pseudocaule da bananeira (Musa sp.) |
| title_sort |
potencial bactericida de biocarvões do pseudocaule da bananeira (musa sp.) |
| publisher |
Universidade Tecnológica Federal do Paraná |
| publishDate |
2023 |
| citation |
BARBOSA, Eduardo Ghiraldi. Potencial bactericida de biocarvões do pseudocaule da bananeira (Musa sp.). 2021. Trabalho de Conclusão de Curso (Bacharelado em Engenharia de Bioprocessos e Biotecnologia) - Universidade Tecnológica Federal do Paraná, Toledo, 2021. |
| url |
http://repositorio.utfpr.edu.br/jspui/handle/1/30653 |
| _version_ |
1805452986484260864 |
| score |
10,814766 |