Efeito do extrato hexânico de Ricinus communis L. sobre esporos de Bacillus thuringiensis
In biological and alternative production systems, plant-based products are used, such as essential oils, plant extracts, as well as entomopathogenic microorganisms, including Bacillus thuringiensis (Bt). Such agents can be applied separately or combined to control insect-pests, one of which may inte...
| Autor principal: | Bianchini Júnior, José Carlos |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
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Agronomia
2023
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http://repositorio.utfpr.edu.br/jspui/handle/1/30943 |
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riut-1-309432023-03-28T06:08:08Z Efeito do extrato hexânico de Ricinus communis L. sobre esporos de Bacillus thuringiensis Hexanic extract efect from Ricinus communis L. on Bacillus thuringiensis spores Bianchini Júnior, José Carlos Silva, Everton Ricardi Lozano da Warmling, Jheniffer Valmira Montanher, Paula Fernandes Silva, Everton Ricardi Lozano da Essências e óleos essenciais Mamona Bacillus thuringiensis Essences and essential oils Castor beans CNPQ::CIENCIAS AGRARIAS::AGRONOMIA In biological and alternative production systems, plant-based products are used, such as essential oils, plant extracts, as well as entomopathogenic microorganisms, including Bacillus thuringiensis (Bt). Such agents can be applied separately or combined to control insect-pests, one of which may interfere with the action of the other. In this sense, the objective of this work was to evaluate the effects of the hexane extract of castor bean (Ricinnus communis) EHRC on Bt spores. For such, the effect of EHRC at 2% was evaluated on Bt spores, simulating the joint application. Tests with spores were performed in vitro, from concentrations of 1x10-6 CFU/mL of each Bt-based product (Crystal®, Dipel® and Xentari®). An aliquot of 60 μL of this suspension was added to Erlenmeyer flasks, containing 10 mL of the 2% EHRC solution. Subsequently, the mixture was inoculated in three Petri dishes, at seven points on the nutrient agar culture surface, and then the CFU/mL per point was quantified. It was observed that the EHRC at 2% reduced the CFU/mL of Bt in all treatments, compared to the respective controls, ranging from 27.18% to 93.18%. Therefore 2% EHRC is not compatible with Bt spores from commercial products Crystal®, Dipel® and Xentari®. Nos sistemas biológicos e alternativos de produção, são utilizados produtos à base de plantas, como de óleos essenciais, extratos vegetais, bem como microrganismos entamopatogênicos, dentre os quais Bacillus thuringiensis (Bt). Tais agentes podem ser aplicados separadamente ou associados para o controle de insetos-pragas, podendo um interferir sobre a ação do outro. Neste sentido, o objetivo deste trabalho foi avaliar os efeitos do extrato hexânico de mamona (Ricinnus communis) EHRC sobre esporos de Bt. Para tal, o efeito do EHRC à 2% foi avaliado sobre esporos de Bt, simulando a aplicação conjunta. Os ensaios com esporos foram realizados in vitro, a partir das concentrações de 1x10-6 UFC/mL de cada produto a base de Bt (Crystal®, Dipel® e Xentari®). Uma alíquota de 60 μL dessa suspensão foi adicionada em frascos de Erlenmeyer, contendo 10 mL da solução de EHRC à 2%. Posteriormente a mistura foi inoculada em três placas-de-Petri, em sete pontos na superfície de cultura ágar nutrientes, e em seguida, quantificado a UFC/mL por ponto. Observou que o EHRC à 2% reduziu as UFC/mL de Bt em todos os tratamentos, comparando-se às respectivas testemunhas, variando de 27,18% a 93,18%. Logo o EHRC à 2% não é compatível com os esporos de Bt dos produtos comerciais Crystal®, Dipel® e Xentari®. 2023-03-27T17:51:19Z 2023-03-27T17:51:19Z 2021-11-23 bachelorThesis BIANCHINI JÚNIOR, José Carlos. Efeito do extrato hexânico de Ricinus communis L. sobre esporos de Bacillus thuringiensis. 2021. Trabalho de Conclusão de Curso (Bacharelado em Agronomia) - Universidade Tecnológica Federal do Paraná, Dois Vizinhos, 2021. http://repositorio.utfpr.edu.br/jspui/handle/1/30943 por openAccess Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Agronomia Dois Vizinhos Brasil Universidade Tecnológica Federal do Paraná UTFPR |
| institution |
Universidade Tecnológica Federal do Paraná |
| collection |
RIUT |
| language |
Português |
| topic |
Essências e óleos essenciais Mamona Bacillus thuringiensis Essences and essential oils Castor beans CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
| spellingShingle |
Essências e óleos essenciais Mamona Bacillus thuringiensis Essences and essential oils Castor beans CNPQ::CIENCIAS AGRARIAS::AGRONOMIA Bianchini Júnior, José Carlos Efeito do extrato hexânico de Ricinus communis L. sobre esporos de Bacillus thuringiensis |
| description |
In biological and alternative production systems, plant-based products are used, such as essential oils, plant extracts, as well as entomopathogenic microorganisms, including Bacillus thuringiensis (Bt). Such agents can be applied separately or combined to control insect-pests, one of which may interfere with the action of the other. In this sense, the objective of this work was to evaluate the effects of the hexane extract of castor bean (Ricinnus communis) EHRC on Bt spores. For such, the effect of EHRC at 2% was evaluated on Bt spores, simulating the joint application. Tests with spores were performed in vitro, from concentrations of 1x10-6 CFU/mL of each Bt-based product (Crystal®, Dipel® and Xentari®). An aliquot of 60 μL of this suspension was added to Erlenmeyer flasks, containing 10 mL of the 2% EHRC solution. Subsequently, the mixture was inoculated in three Petri dishes, at seven points on the nutrient agar culture surface, and then the CFU/mL per point was quantified. It was observed that the EHRC at 2% reduced the CFU/mL of Bt in all treatments, compared to the respective controls, ranging from 27.18% to 93.18%. Therefore 2% EHRC is not compatible with Bt spores from commercial products Crystal®, Dipel® and Xentari®. |
| format |
Trabalho de Conclusão de Curso (Graduação) |
| author |
Bianchini Júnior, José Carlos |
| author_sort |
Bianchini Júnior, José Carlos |
| title |
Efeito do extrato hexânico de Ricinus communis L. sobre esporos de Bacillus thuringiensis |
| title_short |
Efeito do extrato hexânico de Ricinus communis L. sobre esporos de Bacillus thuringiensis |
| title_full |
Efeito do extrato hexânico de Ricinus communis L. sobre esporos de Bacillus thuringiensis |
| title_fullStr |
Efeito do extrato hexânico de Ricinus communis L. sobre esporos de Bacillus thuringiensis |
| title_full_unstemmed |
Efeito do extrato hexânico de Ricinus communis L. sobre esporos de Bacillus thuringiensis |
| title_sort |
efeito do extrato hexânico de ricinus communis l. sobre esporos de bacillus thuringiensis |
| publisher |
Agronomia |
| publishDate |
2023 |
| citation |
BIANCHINI JÚNIOR, José Carlos. Efeito do extrato hexânico de Ricinus communis L. sobre esporos de Bacillus thuringiensis. 2021. Trabalho de Conclusão de Curso (Bacharelado em Agronomia) - Universidade Tecnológica Federal do Paraná, Dois Vizinhos, 2021. |
| url |
http://repositorio.utfpr.edu.br/jspui/handle/1/30943 |
| _version_ |
1810427659501436928 |
| score |
10,814766 |