Determinação de compostos bioativos de extrato e frações de própolis verde, propriedades biológicas e aplicação em emulsões cosméticas
Propolis is a resinous substance produced by bees, with a varied chemical composition, especially phenolic and flavonoid compounds, which give its biological activities, as antioxidant and antimicrobial. The application of raw materials of natural origin to cosmetics is one of the promising trends i...
| Autor principal: | Arenhart, Deisy Karina |
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| Formato: | Dissertação |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2018
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/3271 |
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| Resumo: |
Propolis is a resinous substance produced by bees, with a varied chemical composition, especially phenolic and flavonoid compounds, which give its biological activities, as antioxidant and antimicrobial. The application of raw materials of natural origin to cosmetics is one of the promising trends in the market, given the potential health benefits and possible efficacy in minimizing the phenomena of degradation during the processing and storage phases. Thus, the objective of this study was to determine the content of bioactive compounds and biological activities of extract and fractions of green propolis, with application in cosmetic emulsions. The crude ethanolic extract (CEE) and, by liquid-liquid extraction, the green propolis hexane (FHEX), ethyl acetate (ACT-F) and aqueous (AQ-F) fractions were obtained. The total phenolic compounds (TFC) and flavonoids (FLA) were determined by spectrophotometry and high performance liquid chromatography (HPLC-DAD). In addition, it was to evaluate antioxidant activity by three methods (DPPH, ABTS e FRAP) and antimicrobial activity by minimum inhibitory concentration (MIC). Evaluating the fraction with better results, A bacterial death curve was constructed to verify the time required for inhibition or microbial death. This fraction was then applied to a nonionic O/W emulsion in order to verify its influence on the stability of the processed product as observed through the preliminary and accelerated stability tests. The best results were obtained in ACT-F, with a mean for TFC of 138.41 mg EAG g-1 and FLA of 85.84 mg EQ g-1 and for antioxidant activity 1160.17 µmol TE g -1 (DPPH), 1023.30 µmol TE g -1 (ABTS) and 2020.22 µmol Fe (II) g-1 (FRAP). The most effective MICs were obtained against Gram-positive bacteria Staphylococcus aureus and Staphylococcus aureus MRSA (5.0 mg mL-1 and 10.0 mg mL-1 , respectively) and against yeast Candida albicans (2.5 mg mL-1 ). By HPLC, 4 compounds were identified: the caffeic and p-coumaric phenolic acids and flavonoid pinocembrina and chrysin, with the highest concentrations of p-coumaric and caffeic acid in ACT-F. In the microbial death kinetics test, all microorganisms showed reduced growth over time in exposure to ACT-F of própolis in MIC and MIC*2, characterizing microbicidal and/or microbiostatic activity. With the incorporation of ACT-F, emulsions with a homogeneous appearance and characteristic odor of propolis were obtained and, subjected to the preliminary stability tests, they remained stable from a physicochemical point of view, indicating compatibility between the components. In the accelerated stability tests, the control emulsions (without preservative) and containing 0.2 % ACT-F did not remain stable during the 60 days, presenting phycico-chemical and/or microbiological alterations However, emulsions containing 2.0 % própolis remained stable throughout the study period, indicating that they have potential application as preservatives of the products analyzed. |
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