Registro fonte: Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)

Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)

Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) known as lesser mealworm is the main insect-plague of poultry houses. It causes nutritional losses, sanitary conditions, discomfort to birds and structural damage to production units. The objective of this study was to evaluate the potentia...

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Autor principal:	Rodrigues, Paulo Roberto
Formato:	Dissertação
Idioma:	Português
Publicado em:	Universidade Tecnológica Federal do Paraná 2019
Assuntos:	Pragas - Controle biológico Entomologia Aviários Pests - Biological control Entomology Aviaries CNPQ::CIENCIAS AGRARIAS::AGRONOMIA Agronomia
Acesso em linha:	http://repositorio.utfpr.edu.br/jspui/handle/1/4394
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spelling	riut-1-43942019-09-11T06:00:51Z Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) Potential of commercial entomopathogenic fungi for the control of Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) Rodrigues, Paulo Roberto Potrich, Michele http://lattes.cnpq.br/6017285848848713 Silva, Everton Ricardi Lozano da http://lattes.cnpq.br/0137021895397436 Potrich, Michele Manfio, Daiara Santos, Juliana Cristina dos Pragas - Controle biológico Entomologia Aviários Pests - Biological control Entomology Aviaries CNPQ::CIENCIAS AGRARIAS::AGRONOMIA Agronomia Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) known as lesser mealworm is the main insect-plague of poultry houses. It causes nutritional losses, sanitary conditions, discomfort to birds and structural damage to production units. The objective of this study was to evaluate the potential of the entomopathogenic fungi Beauveria bassiana (IBCB 66 and Bb2), Metarhizium anisopliae (IBCB 425 and Ma2) and Isaria fumosorosea (If1) in different application methodologies on larvae and adults of A. diaperinus under conditions laboratory and semi-field. In the laboratory, the treatments consisted in the application of 1 mL of the suspensions of the isolates of entomopathogenic fungi (1 × 109 conidia.mL-1). Sterilized distilled water, distilled water sterilized with Tween® (0.01%) and chemical insecticide (cypermethrin+chlorpyrifos+piperonilabutoxide) were used as controls. Bioassay 1) The pathogenicity of the isolates was evaluated on larvae and adults of A. diaperinus and; Bioassay 2) the most promising isolates were incorporated into the poultry feed. The insects were transferred to the experimental units (UEs), being Petri dishes (Ø15 cm) for adults, and acrylic plates with 12 wells (Ø1 cm per well) for larvae, these were allocated in an incubator incubator (27 ± 2 ° C, RH 70 ± 10% and photoperiod of 14h). Each treatment consisted of eight replicates, with 12 larvae and 12 adults per replicate. In semi-field, the treatments that were promising were adopted in Bioassay 1 of the laboratory. As controls, the same ones used in laboratory bioassays were used. Bioassay 1) the application of 1.2 ml of the treatments on the surface of the contact UEs of the insects was tested; Bioassay 2) the incorporation of 10 mL of the treatments in medium to the litter. The UEs consisted of organizer boxes, translucent, with 19L of volume, supplied with 0.003 m3 of litter. These were allocated in an air conditioned room under conditions 27 ± 2 ° C, U.R. 70 ± 10% and photoperiod of 14h. Each treatment consisted of five replicates, with 20 larvae and 20 adults per replicate. The evaluations were performed after 10 days, for laboratory, and with seven and 10 days, for semi-field. The variable-response is mortality. In laboratory, in Bioassay 1, isolates BC2, IBCB 425 and IF1 were pathogenic to larvae of A. diaperinus, causing 40; 52.8 and 33.5% mortality, respectively. For adults, only IBCB 425 isolate was pathogenic, causing a 38.3% mortality. For both Laboratory Bioassay 2 and semi-field bioassays 1 and 2, these were considered to be non-significant. Although three isolates of entomopathogenic fungi (Bb2, IBCB 425 and If1) showed pathogenicity on larvae and adults of A. diaperinus, their virulence was considered low. Thus, it is suggested new studies with different isolates of the species used, as well as other fungi species in order to find an efficient isolate for the biological control of A. diaperinus. Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) conhecido como cascudinho-de-aviário é o principal inseto-praga da avicultura de corte. Causa prejuízos nutricionais, sanitários, desconforto às aves e danos estruturais as unidades de produção. Assim, objetivou-se avaliar o potencial dos fungos entomopatogênicos Beauveria bassiana (IBCB 66 e Bb2), Metarhizium anisopliae (IBCB 425 e Ma2) e Isaria fumosorosea (If1) em metodologias distintas de aplicação sobre larvas e adultos de A. diaperinus, em condições de laboratório e semicampo. Em laboratório, os tratamentos consistiram na aplicação de 1 mL das suspensões dos isolados de fungos entomopatogênicos (1×109 conídios.mL-1). Como testemunhas utilizou-se água destilada esterilizada, água destilada esterilizada com Tween® (0,01%) e inseticida químico (cipermetrina+clorpirifós+but.piperonila). Bioensaio 1) Avaliou-se a patogenicidade dos isolados sobre larvas e adultos de A. diaperinus e; Bioensaio 2) os isolados mais promissores foram incorporados à ração das aves. Os insetos foram transferidos para as unidades experimentais (UEs), sendo placas de Petri (Ø15 cm) para adultos, e placas de acrílico com 12 poços (Ø1 cm por poço) para larvas, estas foram alocadas em estufa incubadora (27 ± 2 °C, U.R. 70 ± 10% e fotoperíodo de 14h). Cada tratamento foi composto por oito repetições, com 12 larvas e 12 adultos por repetição. Em semicampo, foram adotados os tratamentos que foram promissores no Bioensaio 1 de laboratório. Como testemunhas utilizou-se as mesmas adotadas nos bioensaios de laboratório. Bioensaio 1) testou-se a aplicação de 1,2 mL dos tratamentos à superfície das UEs de contato dos insetos e; Bioensaio 2) a incorporação de 10 mL dos tratamentos em meio a cama aviária. As UEs consistiram de caixas organizadoras, translúcidas, com 19L de volume, supridas com 0,003 m3 de cama aviária. Estas foram alocadas em sala climatizada sob condições 27 ± 2 °C, U.R. 70 ± 10% e fotoperíodo de 14h. Cada tratamento foi composto por cinco repetições, com 20 larvas e 20 adultos por repetição. As avaliações foram realizadas após 10 dias, para laboratório, e com sete e 10 dias, para semicampo. Sendo a variável resposta a mortalidade. Em laboratório, no Bioensaio 1, os isolados BC2, IBCB 425 e IF1 foram patogênicos a larvas de A. diaperinus, provocando 40; 52,8 e 33,5% de mortalidade, respectivamente. Para adultos, apenas o isolado IBCB 425 foi patogênico, causando 38,3% de mortalidade. Tanto para Bioensaio 2 de laboratório, quanto para os bioensaios 1 e 2 de semicampo, estes foram considerados não significativos. Embora três isolados de fungos entomopatogênicos (Bb2, IBCB 425 e If1) tenham apresentado patogenicidade sobre larvas e adultos de A. diaperinus, sua virulência foi considerada baixa. Assim, sugere-se novos estudos com diferentes isolados das espécies utilizadas, bem como, de outras espécies de fungos afim de encontrar um isolado eficiente para o controle biológico de A. diaperinus. 2019-09-10T15:04:08Z 2019-09-10T15:04:08Z 2019-02-25 masterThesis RODRIGUES, Paulo Roberto. Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). 2019. 65 f. Dissertação (Mestrado em Agroecossistemas) - Universidade Tecnológica Federal do Paraná, Dois Vizinhos, 2019. http://repositorio.utfpr.edu.br/jspui/handle/1/4394 por openAccess application/pdf Universidade Tecnológica Federal do Paraná Dois Vizinhos Brasil Programa de Pós-Graduação em Agroecossistemas UTFPR
institution	Universidade Tecnológica Federal do Paraná
collection	RIUT
language	Português
topic	Pragas - Controle biológico Entomologia Aviários Pests - Biological control Entomology Aviaries CNPQ::CIENCIAS AGRARIAS::AGRONOMIA Agronomia
spellingShingle	Pragas - Controle biológico Entomologia Aviários Pests - Biological control Entomology Aviaries CNPQ::CIENCIAS AGRARIAS::AGRONOMIA Agronomia Rodrigues, Paulo Roberto Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
description	Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) known as lesser mealworm is the main insect-plague of poultry houses. It causes nutritional losses, sanitary conditions, discomfort to birds and structural damage to production units. The objective of this study was to evaluate the potential of the entomopathogenic fungi Beauveria bassiana (IBCB 66 and Bb2), Metarhizium anisopliae (IBCB 425 and Ma2) and Isaria fumosorosea (If1) in different application methodologies on larvae and adults of A. diaperinus under conditions laboratory and semi-field. In the laboratory, the treatments consisted in the application of 1 mL of the suspensions of the isolates of entomopathogenic fungi (1 × 109 conidia.mL-1). Sterilized distilled water, distilled water sterilized with Tween® (0.01%) and chemical insecticide (cypermethrin+chlorpyrifos+piperonilabutoxide) were used as controls. Bioassay 1) The pathogenicity of the isolates was evaluated on larvae and adults of A. diaperinus and; Bioassay 2) the most promising isolates were incorporated into the poultry feed. The insects were transferred to the experimental units (UEs), being Petri dishes (Ø15 cm) for adults, and acrylic plates with 12 wells (Ø1 cm per well) for larvae, these were allocated in an incubator incubator (27 ± 2 ° C, RH 70 ± 10% and photoperiod of 14h). Each treatment consisted of eight replicates, with 12 larvae and 12 adults per replicate. In semi-field, the treatments that were promising were adopted in Bioassay 1 of the laboratory. As controls, the same ones used in laboratory bioassays were used. Bioassay 1) the application of 1.2 ml of the treatments on the surface of the contact UEs of the insects was tested; Bioassay 2) the incorporation of 10 mL of the treatments in medium to the litter. The UEs consisted of organizer boxes, translucent, with 19L of volume, supplied with 0.003 m3 of litter. These were allocated in an air conditioned room under conditions 27 ± 2 ° C, U.R. 70 ± 10% and photoperiod of 14h. Each treatment consisted of five replicates, with 20 larvae and 20 adults per replicate. The evaluations were performed after 10 days, for laboratory, and with seven and 10 days, for semi-field. The variable-response is mortality. In laboratory, in Bioassay 1, isolates BC2, IBCB 425 and IF1 were pathogenic to larvae of A. diaperinus, causing 40; 52.8 and 33.5% mortality, respectively. For adults, only IBCB 425 isolate was pathogenic, causing a 38.3% mortality. For both Laboratory Bioassay 2 and semi-field bioassays 1 and 2, these were considered to be non-significant. Although three isolates of entomopathogenic fungi (Bb2, IBCB 425 and If1) showed pathogenicity on larvae and adults of A. diaperinus, their virulence was considered low. Thus, it is suggested new studies with different isolates of the species used, as well as other fungi species in order to find an efficient isolate for the biological control of A. diaperinus.
format	Dissertação
author	Rodrigues, Paulo Roberto
author_sort	Rodrigues, Paulo Roberto
title	Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
title_short	Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
title_full	Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
title_fullStr	Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
title_full_unstemmed	Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)
title_sort	potencial de fungos entomopatogênicos comerciais para o controle de alphitobius diaperinus (panzer) (coleoptera: tenebrionidae)
publisher	Universidade Tecnológica Federal do Paraná
publishDate	2019
citation	RODRIGUES, Paulo Roberto. Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). 2019. 65 f. Dissertação (Mestrado em Agroecossistemas) - Universidade Tecnológica Federal do Paraná, Dois Vizinhos, 2019.
url	http://repositorio.utfpr.edu.br/jspui/handle/1/4394
_version_	1805310326191685632
score	10,814766

Potencial de fungos entomopatogênicos comerciais para o controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)

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