Registro fonte: Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route

Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route

The 2,4-D is one of the most important agrochemical in Brasil, since its use increased with the development of 2,4-D resistant crops to the large monocultureover. However, its effect on non-target species is still under study. There are many research studying the effect of 2,4-D in the body, however...

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Autor principal:	Hernandéz, Hilda Vanessa Poquioma
Formato:	Dissertação
Idioma:	Inglês
Publicado em:	Universidade Tecnológica Federal do Paraná 2020
Assuntos:	Stress oxidativo Marcadores bioquímicos Enzimas Herbicidas Bioquímica Oxidative stress Biochemical markers Enzymes Herbicides Biochemistry CNPQ::CIENCIAS BIOLOGICAS Biotecnologia
Acesso em linha:	http://repositorio.utfpr.edu.br/jspui/handle/1/5017
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spelling	riut-1-50172020-06-16T06:01:28Z Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route Efeito bioquímico do 2,4-D em animais: revisão meta-analítica e estudo in vivo por via trófica Hernandéz, Hilda Vanessa Poquioma Ghisi, Nédia de Castilhos https://orcid.org/0000-0001-7616-1618 http://lattes.cnpq.br/4542801151720873 Ribeiro, Ciro Alberto de Oliveira https://orcid.org/0000-0002-3168-9272 http://lattes.cnpq.br/7666734780195647 Ghisi, Nédia de Castilhos https://orcid.org/0000-0001-7616-1618 http://lattes.cnpq.br/4542801151720873 Sado, Ricardo Yuji https://orcid.org/0000-0002-6987-9290 http://lattes.cnpq.br/0983192188463013 Yamamoto, Flávia Yoshie https://orcid.org/0000-0002-6880-8458 http://lattes.cnpq.br/1232172428308410 Stress oxidativo Marcadores bioquímicos Enzimas Herbicidas Bioquímica Oxidative stress Biochemical markers Enzymes Herbicides Biochemistry CNPQ::CIENCIAS BIOLOGICAS Biotecnologia The 2,4-D is one of the most important agrochemical in Brasil, since its use increased with the development of 2,4-D resistant crops to the large monocultureover. However, its effect on non-target species is still under study. There are many research studying the effect of 2,4-D in the body, however all of them use different species of a large range of age, applying the xenobiotic by different routes and using many tissues to analyze the effect of 2,4-D on the enzymatic activity. In some of them, the agrotoxic is applied in combination with other compounds using high concentration for few hours, while in others are used more dilute concentrations of the herbicide pure, for several months. All this information makes it difficult the understanding of the real effect of 2,4-D over the oxidative stress. In order to disentangle all this information, it was don a meta analytici study and an in vivo study in fish. In the first part of this research it was studied the response of CAT, SOD, GST, GST, GR, GPx, and the level of GSH in organisms exposed to 2,4-D, in order to determine if variables such as phylogenetical group, age, exposure route, tissue of study, mixture with other compounds, dose applied and exposure time would be influencing the activity of action of those molecules, as a way to provide more conclusive information.In the second part, Rhamdia quelen was exposed to 2,4-D and there were assessed the same biochemical biomarkers from the fist part. In the meta analytical study it was found a clear difference in the response pattern from mammals and no-mammal animals. In the first group it seen a decrease in the activity of SOD and CAT, while in the second group the opposite is appreciated. The mixture with other additives is not a differential factor, while the age is a factor still unclear, studies using young animals were sparse. The hydric exposure causes increase in CAT, SOD and GST activity, while the oral one a drop in the activity of CAT, SOD, GPx and in the level of GSH. The response of the enzymatic and non-enzymatic molecules differs within the tissues however it is clear that male reproductive organs are more sensible regarding female organs. Finally, while the doses exposition is not determinant factor to increase or decrease the enzymatic activity, as longer the exposition time, lesser the activity of CAT, SOD and GPx is seen, while the GSH increases in larger exposition periods. From the in vivo study, there was found no oxidative stress in R. quelen exposed to doses of 20, 200 and 2000µg/kg for a period of 22 and 42 days. From these last results, corresponded with the data found in the first part of this investigation. Oxidative stress was not determined since the exposure occurred for a short period of time. Conselho Nacional do Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) O 2,4-D é um dos agroquímicos mais importantes do Brasil, pois seu uso aumentou com o desenvolvimento de culturas resistentes ao 2,4-D para a grande monocultura. No entanto, seu efeito sobre espécies não-alvo ainda está sendo estudado. Existem muitas pesquisas estudando o efeito do 2,4-D no organismo, no entanto, todos eles usam espécies diferentes de uma grande faixa etária, aplicando o xenobiótico por diferentes rotas e usando muitos tecidos para analisar o efeito do 2,4-D. D sobre a atividade enzimática. Em alguns deles, o agrotóxico é aplicado em combinação com outros compostos, utilizando alta concentração por algumas horas, enquanto em outros são utilizadas concentrações mais diluídas do herbicida puro, por vários meses. Toda essa informação dificulta a compreensão do efeito real do 2,4-D sobre o estresse oxidativo. Para desmembrar todas essas informações, foi realizado um estudo metaanalítico e um estudo in vivo em peixes. Na primeira parte desta pesquisa, estudouse a resposta de CAT, SOD, GST, GST, GR, GPx e o nível de GSH em organismos expostos ao 2,4-D, a fim de determinar se variáveis como grupo filogenético , idade, via de exposição, tecido do estudo, mistura com outros compostos, dose aplicada e tempo de exposição influenciam a atividade de ação dessas moléculas, como forma de fornecer informações mais conclusivas. Na segunda parte, Rhamdia quelen foi exposto ao 2,4-D e foram avaliados os mesmos biomarcadores bioquímicos da primeira parte. No estudo meta-analítico, foi encontrada uma clara diferença no padrão de resposta de mamíferos e animais não mamíferos. No primeiro grupo, houve uma diminuição na atividade de SOD e CAT, enquanto no segundo grupo, o oposto é apreciado. A mistura com outros aditivos não é um fator diferencial, enquanto a idade ainda é um fator incerto, estudos com animais jovens foram escassos. A exposição hídrica causa aumento da atividade de CAT, SOD e GST, enquanto a exposição oral diminui a atividade de CAT, SOD, GPx e o nível de GSH. A resposta das moléculas enzimáticas e não enzimáticas difere dentro dos tecidos, porém é claro que os órgãos reprodutores masculinos são mais sensíveis em relação aos órgãos femininos. Finalmente, enquanto a exposição das doses não é fator determinante para aumentar ou diminuir a atividade enzimática, quanto maior o tempo de exposição, menor é a atividade de CAT, SOD e GPx, enquanto o GSH aumenta em períodos de exposição maiores. No estudo in vivo, não foi encontrado estresse oxidativo em R. quelen exposto a doses de 20, 200 e 2000µg / kg por um período de 22 e 42 dias. A partir de esses últimos resutaldos pode se conferir os dados encontrados na primeira parte de esta investigação. Não foi determinado estresse oxidativo já que a exposição fue por courto período de tempo. 2020-06-15T20:00:02Z 2020-06-15T20:00:02Z 2020-02-28 masterThesis HERNANDÉZ, Hilda Vanessa Poquioma. Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route. 2020. Thesis (Master's Degree) - Universidade Tecnológica Federal do Paraná, Ponta Grossa, 2020. http://repositorio.utfpr.edu.br/jspui/handle/1/5017 eng openAccess application/pdf Universidade Tecnológica Federal do Paraná Dois Vizinhos Ponta Grossa Brasil Programa de Pós-Graduação em Biotecnologia UTFPR
institution	Universidade Tecnológica Federal do Paraná
collection	RIUT
language	Inglês
topic	Stress oxidativo Marcadores bioquímicos Enzimas Herbicidas Bioquímica Oxidative stress Biochemical markers Enzymes Herbicides Biochemistry CNPQ::CIENCIAS BIOLOGICAS Biotecnologia
spellingShingle	Stress oxidativo Marcadores bioquímicos Enzimas Herbicidas Bioquímica Oxidative stress Biochemical markers Enzymes Herbicides Biochemistry CNPQ::CIENCIAS BIOLOGICAS Biotecnologia Hernandéz, Hilda Vanessa Poquioma Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route
description	The 2,4-D is one of the most important agrochemical in Brasil, since its use increased with the development of 2,4-D resistant crops to the large monocultureover. However, its effect on non-target species is still under study. There are many research studying the effect of 2,4-D in the body, however all of them use different species of a large range of age, applying the xenobiotic by different routes and using many tissues to analyze the effect of 2,4-D on the enzymatic activity. In some of them, the agrotoxic is applied in combination with other compounds using high concentration for few hours, while in others are used more dilute concentrations of the herbicide pure, for several months. All this information makes it difficult the understanding of the real effect of 2,4-D over the oxidative stress. In order to disentangle all this information, it was don a meta analytici study and an in vivo study in fish. In the first part of this research it was studied the response of CAT, SOD, GST, GST, GR, GPx, and the level of GSH in organisms exposed to 2,4-D, in order to determine if variables such as phylogenetical group, age, exposure route, tissue of study, mixture with other compounds, dose applied and exposure time would be influencing the activity of action of those molecules, as a way to provide more conclusive information.In the second part, Rhamdia quelen was exposed to 2,4-D and there were assessed the same biochemical biomarkers from the fist part. In the meta analytical study it was found a clear difference in the response pattern from mammals and no-mammal animals. In the first group it seen a decrease in the activity of SOD and CAT, while in the second group the opposite is appreciated. The mixture with other additives is not a differential factor, while the age is a factor still unclear, studies using young animals were sparse. The hydric exposure causes increase in CAT, SOD and GST activity, while the oral one a drop in the activity of CAT, SOD, GPx and in the level of GSH. The response of the enzymatic and non-enzymatic molecules differs within the tissues however it is clear that male reproductive organs are more sensible regarding female organs. Finally, while the doses exposition is not determinant factor to increase or decrease the enzymatic activity, as longer the exposition time, lesser the activity of CAT, SOD and GPx is seen, while the GSH increases in larger exposition periods. From the in vivo study, there was found no oxidative stress in R. quelen exposed to doses of 20, 200 and 2000µg/kg for a period of 22 and 42 days. From these last results, corresponded with the data found in the first part of this investigation. Oxidative stress was not determined since the exposure occurred for a short period of time.
format	Dissertação
author	Hernandéz, Hilda Vanessa Poquioma
author_sort	Hernandéz, Hilda Vanessa Poquioma
title	Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route
title_short	Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route
title_full	Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route
title_fullStr	Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route
title_full_unstemmed	Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route
title_sort	biochemical effect of 2,4-d in animals: meta-analytic review, and in vivo study through trophic route
publisher	Universidade Tecnológica Federal do Paraná
publishDate	2020
citation	HERNANDÉZ, Hilda Vanessa Poquioma. Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route. 2020. Thesis (Master's Degree) - Universidade Tecnológica Federal do Paraná, Ponta Grossa, 2020.
url	http://repositorio.utfpr.edu.br/jspui/handle/1/5017
_version_	1805316644050829312
score	10,814766

Biochemical effect of 2,4-D in animals: meta-analytic review, and in vivo study through trophic route

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