Registro fonte: Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja

Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja

Fusarium oxysporum is the causative agent of Fusarium wilt in tomato, one of the ways to control the disease is through chemical treatment of seeds, however the present work seeks an alternative solution to chemical treatment, using the purging technique with essential oils. The objective of the wor...

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Autor principal:	Russiano, Carlos Guilherme dos Santos
Formato:	Dissertação
Idioma:	Português
Publicado em:	Universidade Tecnológica Federal do Paraná 2020
Assuntos:	Tomate Essências e óleos essenciais Germinação Sementes Fusarium oxysporum Tomatoes Essences and essential oils Germination Seeds CNPQ::CIENCIAS AGRARIAS Agronomia
Acesso em linha:	http://repositorio.utfpr.edu.br/jspui/handle/1/5084
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spelling	riut-1-50842020-07-21T06:01:03Z Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja Volatility of essential oils in the control of Fusarium oxysporum in cherry tomato seeds Russiano, Carlos Guilherme dos Santos Moraes, Pedro Valério Dutra de https://orcid.org/0000-0002-5278-1377 http://lattes.cnpq.br/2831331040980295 Mazaro, Sérgio Miguel https://orcid.org/0000-0003-2787-9409 http://lattes.cnpq.br/4271660992059925 Manteli, Claudia https://orcid.org/0000-0001-8797-2889 http://lattes.cnpq.br/5206965399284783 Vismara, Lilian de Souza https://orcid.org/0000-0002-2879-1401 http://lattes.cnpq.br/3439514004356616 Moraes, Pedro Valério Dutra de https://orcid.org/0000-0002-5278-1377 http://lattes.cnpq.br/2831331040980295 Tomate Essências e óleos essenciais Germinação Sementes Fusarium oxysporum Tomatoes Essences and essential oils Germination Seeds CNPQ::CIENCIAS AGRARIAS Agronomia Fusarium oxysporum is the causative agent of Fusarium wilt in tomato, one of the ways to control the disease is through chemical treatment of seeds, however the present work seeks an alternative solution to chemical treatment, using the purging technique with essential oils. The objective of the work is to evaluate the effect of purge with essential oils of tea tree, chia, citronella, lavender, anise and clove lavender in cherry tomato seeds contaminated with Fusarium oxysporum. The experiments were carried out at UTFPR, Dois Vizinhos campus, in the phytopathology and seed laboratories. Organic cherry tomato seeds, batch 118074-001-52 from ISLA were used. In all experiments, a completely randomized design with four replications was used. After contact of the seeds with the inoculum in Petri dishes for a period of 24 hours, followed by purging them with essential oils, the gerbox germination test was performed with 25 seeds in each experimental unit, for a period of 8 days, checking the variables IVG,% G, TMG. With the same seeds, mycelial growth was evaluated, in order to verify its influence on the phytopathogen morphology, for this, a seed from each treatment was placed in the center of a Petri dish, where mycelial growth was measured; and then samples of each treatment were observed in a scanning electron microscope. After confirming the best treatment, through the results obtained, tea tree oil was again submitted to the germination test, where 25 seeds of each treatment composed of the cherry tomato seeds in contact with the pathogen in the petri dishes for 24 hours and purged for another 24 hours with tea tree essential oil in closed crucibles; plus the positive control in which seeds without inoculum were used, and the negative control, containing only the seeds with the inoculum of F. oxysporum; in which every three days four gerboxes from each treatment were opened to check the percentage of germination of the seeds, number of abnormal seeds and percentage of presence of mycelium in the seeds, and then collected in plastic bags and taken to the freezer to later proceed the resistance induction tests in seedlings, through the analysis of chitinase, glucanase and total proteins. The results obtained were submitted to the Tukey test (P≤0.05), and the regression analysis, through the computational tool Genes. The final data showed that tea tree oil can inhibit the mycelial growth of F. oxysporum, without harming the germination of cherry tomato seeds. Subsequent tests with this oil also demonstrate that with the oil there is a reduction of mycelium in the seeds and a reduction of abnormal seedlings in comparison with the negative control. There was no significant difference between the variables tested for resistance induction. Fusarium oxysporum é o agente causador da murcha de Fusarium em tomateiro, uma das formas de controle da doença é através do tratamento químico de sementes, porém o presente trabalho busca uma solução alternativa ao tratamento químico, utilizando a técnica de expurgo com óleos essenciais. O objetivo do trabalho é avaliar o efeito do expurgo com óleos essenciais de melaleuca, chia, citronela, lavanda, alfavaca anis e alfavaca cravo em sementes de tomate cereja contaminados com Fusarium oxysporum. Os experimentos foram realizados na UTFPR, campus Dois Vizinhos, nos laboratórios de fitopatologia e laboratório de sementes. Utilizou-se sementes orgânicas de tomate cereja, lote118074-001-52 da ISLA. Em todos os experimentos foram utilizados o delineamento inteiramente ao acaso com quatro repetições. Após contato das sementes com o inóculo em placas de Petri por um período de 24 horas, seguido por expurgo das mesmas com os óleos essenciais, foi feito o teste de germinação em gerbox com 25 sementes em cada unidade experimental, por um período de 8 dias, verificando as variáveis IVG, %G, TMG. Com as mesmas sementes, o crescimento micelial foi avaliado, a fim de verificar sua influência na morfologia do fitopatógeno, para isso, uma semente de cada tratamento foi disposta no centro de uma placa de Petri, onde foi medido o crescimento micelial; e em seguida amostras de cada tratamento foram observadas em microscópio eletrônico de varredura. Após constatar o melhor tratamento, por meio dos resultados obtidos, o óleo de melaleuca foi submetido novamente ao teste de germinação, onde 25 sementes de cada tratamento composto pelas sementes de tomate cereja em contato com o patógeno nas placas de petri por 24 horas e expurgadas por mais 24 horas com óleo essencial de melaleuca em cadinhos fechados; mais a testemunha positiva em que foram usadas sementes isentas de inóculo, e a testemunha negativa, contendo apenas as sementes com o inóculo de F. oxysporum; em que de três em três dias quatro gerbox de cada tratamento eram abertos para verificar a porcentagem de germinação das sementes, número de sementes anormais e porcentagem de presença de micélio nas sementes, e em seguida coletadas em sacos plásticos e levadas ao congelador para posteriormente proceder os testes de indução de resistência nas plântulas, por meio da análise de quitinase, glucanase e proteínas totais. Os resultados obtidos foram submetidos ao Teste de Tukey (P≤0,05), e a análise de regressão, através da ferramenta computacional Genes. Os dados finais mostraram que o óleo de melaleuca pode inibir o crescimento micelial de F. oxysporum, sem prejudicar a germinação das sementes de tomate cereja. Os testes subsequentes com este óleo, também demonstram que com o óleo há a redução de micélio nas sementes e redução de plântulas anormais em comparação com a testemunha negativa. Não ocorreu diferença significativa entre as variáveis testadas para indução de resistência. 2020-07-20T12:57:24Z 2020-07-20T12:57:24Z 2020-03-09 masterThesis RUSSIANO, Carlos Guilherme dos Santos. Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja. 2020. Dissertação (Mestrado em Agroecossistemas) - Universidade Tecnológica Federal do Paraná, Dois Vizinhos, 2020. http://repositorio.utfpr.edu.br/jspui/handle/1/5084 por openAccess application/pdf Universidade Tecnológica Federal do Paraná Dois Vizinhos Brasil Programa de Pós-Graduação em Agroecossistemas UTFPR
institution	Universidade Tecnológica Federal do Paraná
collection	RIUT
language	Português
topic	Tomate Essências e óleos essenciais Germinação Sementes Fusarium oxysporum Tomatoes Essences and essential oils Germination Seeds CNPQ::CIENCIAS AGRARIAS Agronomia
spellingShingle	Tomate Essências e óleos essenciais Germinação Sementes Fusarium oxysporum Tomatoes Essences and essential oils Germination Seeds CNPQ::CIENCIAS AGRARIAS Agronomia Russiano, Carlos Guilherme dos Santos Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja
description	Fusarium oxysporum is the causative agent of Fusarium wilt in tomato, one of the ways to control the disease is through chemical treatment of seeds, however the present work seeks an alternative solution to chemical treatment, using the purging technique with essential oils. The objective of the work is to evaluate the effect of purge with essential oils of tea tree, chia, citronella, lavender, anise and clove lavender in cherry tomato seeds contaminated with Fusarium oxysporum. The experiments were carried out at UTFPR, Dois Vizinhos campus, in the phytopathology and seed laboratories. Organic cherry tomato seeds, batch 118074-001-52 from ISLA were used. In all experiments, a completely randomized design with four replications was used. After contact of the seeds with the inoculum in Petri dishes for a period of 24 hours, followed by purging them with essential oils, the gerbox germination test was performed with 25 seeds in each experimental unit, for a period of 8 days, checking the variables IVG,% G, TMG. With the same seeds, mycelial growth was evaluated, in order to verify its influence on the phytopathogen morphology, for this, a seed from each treatment was placed in the center of a Petri dish, where mycelial growth was measured; and then samples of each treatment were observed in a scanning electron microscope. After confirming the best treatment, through the results obtained, tea tree oil was again submitted to the germination test, where 25 seeds of each treatment composed of the cherry tomato seeds in contact with the pathogen in the petri dishes for 24 hours and purged for another 24 hours with tea tree essential oil in closed crucibles; plus the positive control in which seeds without inoculum were used, and the negative control, containing only the seeds with the inoculum of F. oxysporum; in which every three days four gerboxes from each treatment were opened to check the percentage of germination of the seeds, number of abnormal seeds and percentage of presence of mycelium in the seeds, and then collected in plastic bags and taken to the freezer to later proceed the resistance induction tests in seedlings, through the analysis of chitinase, glucanase and total proteins. The results obtained were submitted to the Tukey test (P≤0.05), and the regression analysis, through the computational tool Genes. The final data showed that tea tree oil can inhibit the mycelial growth of F. oxysporum, without harming the germination of cherry tomato seeds. Subsequent tests with this oil also demonstrate that with the oil there is a reduction of mycelium in the seeds and a reduction of abnormal seedlings in comparison with the negative control. There was no significant difference between the variables tested for resistance induction.
format	Dissertação
author	Russiano, Carlos Guilherme dos Santos
author_sort	Russiano, Carlos Guilherme dos Santos
title	Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja
title_short	Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja
title_full	Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja
title_fullStr	Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja
title_full_unstemmed	Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja
title_sort	volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja
publisher	Universidade Tecnológica Federal do Paraná
publishDate	2020
citation	RUSSIANO, Carlos Guilherme dos Santos. Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja. 2020. Dissertação (Mestrado em Agroecossistemas) - Universidade Tecnológica Federal do Paraná, Dois Vizinhos, 2020.
url	http://repositorio.utfpr.edu.br/jspui/handle/1/5084
_version_	1805306366358716416
score	10,814766

Volatilidade de óleos essências no controle de fusarium oxysporum em sementes de tomate cereja

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