Teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (Plantago major)
The study of plants that are used in folk medicine is of great importance to enhance its effectiveness and safety. In order to strengthen research with plants of popular use, the Ministry of Health published the list of RENISUS. In this list we find the tansagem (Plantago major), which is used in fo...
| Autor principal: | Santos, Katlyn Bazoli dos |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
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Universidade Tecnológica Federal do Paraná
2020
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riut-1-55022020-11-06T13:36:37Z Teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (Plantago major) Bioactive compounds contents and antioxidant capacity of the leaves of the tansagem (Plantago major) Santos, Katlyn Bazoli dos Tonin, Lilian Tatiani Dusman Tonin, Lilian Tatiani Dusman Bonafe, Elton Guntendorfer Suzuki, Rúbia Michele Medicina popular Plantas medicinais Antioxidantes Medicine, Popular Medicinal plants Antioxidants CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA The study of plants that are used in folk medicine is of great importance to enhance its effectiveness and safety. In order to strengthen research with plants of popular use, the Ministry of Health published the list of RENISUS. In this list we find the tansagem (Plantago major), which is used in folk medicine as diuretic, antidiarrheal, expectorant, hemostatic and healing, and is used against infections of the upper respiratory tract (pharyngitis, tonsillitis, stomatitis), chronic bronchitis and as auxiliary In the treatment of peptic ulcers, among others. Studies on the species indicated the presence of phenolic compounds, flavonoids, iridoids, among others. In the present work the drying of P. major leaves was carried out at temperatures of 40, 60 and 80 ºC. The drying process was monitored until the mass of the material reached a constant value. The drying time was inversely proportional to the temperature, which was already expected. It was possible to remove about 92% of the moisture at all temperatures in short drying times. The contents of phenolic compounds were quantified and the highest value was EtOH/H2O 30% extract from drying at 40°C with 0.7459 mg EAG 100 g-1 sample. The conditions with the highest total flavonoid content were extractions with MeOH/H2O 5% solvents with dry tannage at 40 and 60oC and EtOH/H2O 5% with drying at 80oC. For the free radical sequestration method DPPH at a concentration of 10000 μg mL-1 the extracts presented rapid kinetics and percent inhibition of the radical greater than 92% in the time of 30 minutes. At the concentration of 1000 μg mL-1 in the time of 30 minutes, the extracts that presented the highest percentage of inhibition of the DPPH radical were EtOH/H2O 30% and MeOH/H2O 5%, with rapid kinetics. For the extracts activity against ABTS, at the concentration of 10000 μg mL-1 the four extracts of the drying at 40oC presented higher percentages of inhibition of the radical. At the time of 30 minutes of reaction, the percentages of antioxidant activity ranged from 57.06% to 63.67%. Thus, by analyzing all the results obtained, it is possible to propose that the drying of the tansagem be carried out at a temperature of 40°C, and the extract prepared with MeOH/H2O5% or EtOH/H2O30%, and considering the lower toxicity of the Ethanol, it is used as the best solvent to extract its bioactive compounds O estudo de plantas que são utilizadas na medicina popular é de grande importância para aprimorar sua eficácia e segurança. Com o intuito de fortalecer as pesquisas com plantas de uso popular, o Ministério da Saúde publicou a lista do RENISUS. Nesta lista encontra-se a tansagem (Plantago major), que é utilizada na medicina popular como diurética, antidiarreica, expectorante, hemostática e cicatrizante, sendo empregada contra infecções das vias respiratórias superiores (faringite, amigdalite, estomatite), bronquite crônica e como auxiliar no tratamento de úlceras pépticas, entre outras. Estudos sobre a espécie indicaram a presença de compostos fenólicos, flavonoides, iridoides, entre outros. O presente trabalho teve como objetivo a secagem das folhas de P. major foi realizada nas temperaturas de 40, 60 e 80 ºC. O processo de secagem foi monitorado até que a massa do material chegasse a um valor constante. O tempo de secagem foi inversamente proporcional à temperatura, o que já era esperado. Foi possível remover cerca de 92% da umidade para todas as temperaturas, em tempos curtos de secagem.Os teores de compostos fenólicos foram quantificados e o maior valor foi para o extrato EtOH/H2O 30% da secagem a 40 ºC com 0,7459 mg EAG 100 g-1de amostra. As condições que apresentaram maior teor de flavonoides totais foram as extrações realizadas com os solventes MeOH/H2O 5% com a tansagem seca a 40 e 60oC eEtOH/H2O 5% com a tansagem seca a 80oC.Para o método de sequestro do radical livre DPPH em concentração de 10.000 µg mL-1os extratos apresentaram cinética rápida e porcentagem de inibição do radical superior a 92% no tempo de 30 minutos. Na concentração de 1.000 µg mL-1 no tempo de 30 minutos, os extratos que apresentaram maior porcentagem de inibição do radical DPPH foram o EtOH/H2O 30% e MeOH/H2O 5%, com cinética rápida.Para a atividade dos extratos frente o ABTS, na concentração de 10.000 µg mL-1os quatro extratos da secagem a 40oC apresentaram maiores percentuais de inibição do radical.No tempo de 30 minutos de reação, as porcentagens de atividade antioxidante variaram de 57,06% a 63,67%. Dessa forma, analisando todos os resultados obtidos, é possível propor que a secagem do tansagem seja realizada a uma temperatura de 40°C, e o extrato preparado com MeOH/H2O 5% ou EtOH/H2O 30%, e considerando a menor toxicidade do etanol, este seja utilizado como melhor solvente extrator dos seus compostos bioativos. 2020-11-06T13:36:37Z 2020-11-06T13:36:37Z 2017-06-22 bachelorThesis SANTOS, Katlyn Bazoli dos. Teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (Plantago major). 2017. 63 f. Trabalho de Conclusão de Curso (Graduação) — Universidade Tecnológica Federal do Paraná, Apucarana, 2017. http://repositorio.utfpr.edu.br/jspui/handle/1/5502 por openAccess application/pdf Universidade Tecnológica Federal do Paraná Apucarana Brasil Licenciatura em Química UTFPR |
| institution |
Universidade Tecnológica Federal do Paraná |
| collection |
RIUT |
| language |
Português |
| topic |
Medicina popular Plantas medicinais Antioxidantes Medicine, Popular Medicinal plants Antioxidants CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
| spellingShingle |
Medicina popular Plantas medicinais Antioxidantes Medicine, Popular Medicinal plants Antioxidants CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA Santos, Katlyn Bazoli dos Teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (Plantago major) |
| description |
The study of plants that are used in folk medicine is of great importance to enhance its effectiveness and safety. In order to strengthen research with plants of popular use, the Ministry of Health published the list of RENISUS. In this list we find the tansagem (Plantago major), which is used in folk medicine as diuretic, antidiarrheal, expectorant, hemostatic and healing, and is used against infections of the upper respiratory tract (pharyngitis, tonsillitis, stomatitis), chronic bronchitis and as auxiliary In the treatment of peptic ulcers, among others. Studies on the species indicated the presence of phenolic compounds, flavonoids, iridoids, among others. In the present work the drying of P. major leaves was carried out at temperatures of 40, 60 and 80 ºC. The drying process was monitored until the mass of the material reached a constant value. The drying time was inversely proportional to the temperature, which was already expected. It was possible to remove about 92% of the moisture at all temperatures in short drying times. The contents of phenolic compounds were quantified and the highest value was EtOH/H2O 30% extract from drying at 40°C with 0.7459 mg EAG 100 g-1 sample. The conditions with the highest total flavonoid content were extractions with MeOH/H2O 5% solvents with dry tannage at 40 and 60oC and EtOH/H2O 5% with drying at 80oC. For the free radical sequestration method DPPH at a concentration of 10000 μg mL-1 the extracts presented rapid kinetics and percent inhibition of the radical greater than 92% in the time of 30 minutes. At the concentration of 1000 μg mL-1 in the time of 30 minutes, the extracts that presented the highest percentage of inhibition of the DPPH radical were EtOH/H2O 30% and MeOH/H2O 5%, with rapid kinetics. For the extracts activity against ABTS, at the concentration of 10000 μg mL-1 the four extracts of the drying at 40oC presented higher percentages of inhibition of the radical. At the time of 30 minutes of reaction, the percentages of antioxidant activity ranged from 57.06% to 63.67%. Thus, by analyzing all the results obtained, it is possible to propose that the drying of the tansagem be carried out at a temperature of 40°C, and the extract prepared with MeOH/H2O5% or EtOH/H2O30%, and considering the lower toxicity of the Ethanol, it is used as the best solvent to extract its bioactive compounds |
| format |
Trabalho de Conclusão de Curso (Graduação) |
| author |
Santos, Katlyn Bazoli dos |
| author_sort |
Santos, Katlyn Bazoli dos |
| title |
Teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (Plantago major) |
| title_short |
Teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (Plantago major) |
| title_full |
Teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (Plantago major) |
| title_fullStr |
Teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (Plantago major) |
| title_full_unstemmed |
Teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (Plantago major) |
| title_sort |
teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (plantago major) |
| publisher |
Universidade Tecnológica Federal do Paraná |
| publishDate |
2020 |
| citation |
SANTOS, Katlyn Bazoli dos. Teores de compostos bioativos e capacidade antioxidante das folhas da tansagem (Plantago major). 2017. 63 f. Trabalho de Conclusão de Curso (Graduação) — Universidade Tecnológica Federal do Paraná, Apucarana, 2017. |
| url |
http://repositorio.utfpr.edu.br/jspui/handle/1/5502 |
| _version_ |
1805316652714164224 |
| score |
10,814766 |