Estudo visando à obtenção de micropartículas e géis de quitosana para liberação controlada de substâncias bioativas
In the search for new pharmacologically active compounds, several studies have sought to minimize the unwanted effects of non-steroidal anti-inflammatory, is the search for a more comfortable and efficient form of management , but also provide the patient with a more potent drug with fewer effects s...
| Autor principal: | Primo, Julia de Oliveira |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2020
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/6129 |
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| Resumo: |
In the search for new pharmacologically active compounds, several studies have sought to minimize the unwanted effects of non-steroidal anti-inflammatory, is the search for a more comfortable and efficient form of management , but also provide the patient with a more potent drug with fewer effects side. Thereby seeking to contribute to advances in the development of new drugs, this study investigated the controlled release of naproxen and eugenyl and thymyl naproxenates. For this, gels, microparticles and chitosan blends were prepared. In order to obtain these gels, a sample of chitosan, with a degree of deacetylation of
75-85%, was acetylated in heterogeneous medium using pyridine and acetic anhydride. The structural characteristics of the obtained product was analyzed by infrared spectroscopy, 13C nuclear magnetic resonance and acetylation degree was determined by a volumetric method (ASTM D871-72). The results suggest a N-acetylation degree of 99.5%, in agreement with the literature value. Through of the N-acetylchitosan solubilization in a mixture of N,Ndimethylformamide/5% LiCl were obtained gels that showed a high stability, even after washing with distilled water. N-acetylchitosan gels and of N-acetylchitosan/cellulose acetate blends with bioactive substances (naproxen, eugenyl and thymyl naproxenates) were produced using the casting method. The morphological characteristics of the fracture surfaces of the pure N-acetylchitosan gels were investigated by scanning electron microscopy. The morphology revealed a smooth surface and shows a dense and uniform film. The blends obtained are opaque indicating immiscibility of the system, however with the addition of bioactive substances the system became transparent, suggesting that naproxen acts as compatibilizer. The release kinetics of the bioactive substances incorporated in Nacetylchitosan gels were evaluated by UV-Vis spectroscopy at predetermined periods of time. For the N-acetylchitosan/naproxen system, in 8 minutes, was observed to release 90% of naproxen. As for the blends there was a more controlled release, by releasing 20% of naproxen in 5 minutes. The microparticles were obtained by different methods, the particles showed different morphologies, with an average size of 1 mm. The ALG-QT particles prepared with naproxen, have similar morphology that pure microparticles. The release study showed that the microparticles prepared by the indirect method, initially showed a more controlled release profile. The results of this study indicate that N-acetylchitosan gels are promising systems for transdermal drug delivery, in real conditions (in vivo), may be used to improve/optimize the time of action and maintain therapeutic concentration of bioactive substances. |
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