Fluorescence-based lab-on-chip spot design for improved signal detection

This work presents a new perspective for fluorescence signal detection using specific optics on Lab-on-Chip devices. An apparatus was designed and implemented in order to assess the performance of a fluorescence technique using different detection spot configurations, using the chip itself as a wave...

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Principais autores: Bassani, Thiago, Dias, Philipe, Branco, Gilberto, Silva, Wilson José da, Vieira Neto, Hugo, Schneider, Fábio Kurt
Formato: Trabalho Apresentado em Evento
Idioma: Inglês
Publicado em: Curitiba 2013
Assuntos:
Acesso em linha: http://repositorio.utfpr.edu.br/jspui/handle/1/655
http://dx.doi.org/10.1109/EMBC.2012.6345988
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spelling riut-1-6552015-03-07T06:11:40Z Fluorescence-based lab-on-chip spot design for improved signal detection Bassani, Thiago Dias, Philipe Branco, Gilberto Silva, Wilson José da Vieira Neto, Hugo Schneider, Fábio Kurt Fluorescência Imunofluorescência Óptica Fluorescence Immunofluorescence Optics This work presents a new perspective for fluorescence signal detection using specific optics on Lab-on-Chip devices. An apparatus was designed and implemented in order to assess the performance of a fluorescence technique using different detection spot configurations, using the chip itself as a waveguide for illumination. The experiments conducted investigate the influence of the dimensions – diameter and height – of the spot on the amplitude of the detection output signal. Results show that the configuration of optical interfaces must be considered in order to improve detection output, or to be able to detect less fluorophore molecules in the spot. 5000 2013-11-21T22:10:39Z 2012 conferenceObject BASSANI, Thiago et al. Fluorescence-based lab-on-chip spot design for improved signal detection. In: ANNUAL INTERNATIONALCONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, 34., 2012, San Diego. Anais eletrônicos… San Diego, 2012. Disponível em: <http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6345988>. Acesso em: 16 jul. 2013. 978-1-4244-4120-4 1557-170X http://repositorio.utfpr.edu.br/jspui/handle/1/655 http://dx.doi.org/10.1109/EMBC.2012.6345988 eng Annual International Conference of the IEEE Engineering in Medicine and Biology Society http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6345988&tag=1 application/pdf Curitiba
institution Universidade Tecnológica Federal do Paraná
collection RIUT
language Inglês
topic Fluorescência
Imunofluorescência
Óptica
Fluorescence
Immunofluorescence
Optics
spellingShingle Fluorescência
Imunofluorescência
Óptica
Fluorescence
Immunofluorescence
Optics
Bassani, Thiago
Dias, Philipe
Branco, Gilberto
Silva, Wilson José da
Vieira Neto, Hugo
Schneider, Fábio Kurt
Fluorescence-based lab-on-chip spot design for improved signal detection
description This work presents a new perspective for fluorescence signal detection using specific optics on Lab-on-Chip devices. An apparatus was designed and implemented in order to assess the performance of a fluorescence technique using different detection spot configurations, using the chip itself as a waveguide for illumination. The experiments conducted investigate the influence of the dimensions – diameter and height – of the spot on the amplitude of the detection output signal. Results show that the configuration of optical interfaces must be considered in order to improve detection output, or to be able to detect less fluorophore molecules in the spot.
format Trabalho Apresentado em Evento
author Bassani, Thiago
Dias, Philipe
Branco, Gilberto
Silva, Wilson José da
Vieira Neto, Hugo
Schneider, Fábio Kurt
author_sort Bassani, Thiago
title Fluorescence-based lab-on-chip spot design for improved signal detection
title_short Fluorescence-based lab-on-chip spot design for improved signal detection
title_full Fluorescence-based lab-on-chip spot design for improved signal detection
title_fullStr Fluorescence-based lab-on-chip spot design for improved signal detection
title_full_unstemmed Fluorescence-based lab-on-chip spot design for improved signal detection
title_sort fluorescence-based lab-on-chip spot design for improved signal detection
publisher Curitiba
publishDate 2013
citation BASSANI, Thiago et al. Fluorescence-based lab-on-chip spot design for improved signal detection. In: ANNUAL INTERNATIONALCONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, 34., 2012, San Diego. Anais eletrônicos… San Diego, 2012. Disponível em: <http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6345988>. Acesso em: 16 jul. 2013.
978-1-4244-4120-4
1557-170X
url http://repositorio.utfpr.edu.br/jspui/handle/1/655
http://dx.doi.org/10.1109/EMBC.2012.6345988
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score 10,814766