Fluorescence-based lab-on-chip spot design for improved signal detection
This work presents a new perspective for fluorescence signal detection using specific optics on Lab-on-Chip devices. An apparatus was designed and implemented in order to assess the performance of a fluorescence technique using different detection spot configurations, using the chip itself as a wave...
Principais autores: | Bassani, Thiago, Dias, Philipe, Branco, Gilberto, Silva, Wilson José da, Vieira Neto, Hugo, Schneider, Fábio Kurt |
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Formato: | Trabalho Apresentado em Evento |
Idioma: | Inglês |
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Curitiba
2013
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http://repositorio.utfpr.edu.br/jspui/handle/1/655 http://dx.doi.org/10.1109/EMBC.2012.6345988 |
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riut-1-6552015-03-07T06:11:40Z Fluorescence-based lab-on-chip spot design for improved signal detection Bassani, Thiago Dias, Philipe Branco, Gilberto Silva, Wilson José da Vieira Neto, Hugo Schneider, Fábio Kurt Fluorescência Imunofluorescência Óptica Fluorescence Immunofluorescence Optics This work presents a new perspective for fluorescence signal detection using specific optics on Lab-on-Chip devices. An apparatus was designed and implemented in order to assess the performance of a fluorescence technique using different detection spot configurations, using the chip itself as a waveguide for illumination. The experiments conducted investigate the influence of the dimensions – diameter and height – of the spot on the amplitude of the detection output signal. Results show that the configuration of optical interfaces must be considered in order to improve detection output, or to be able to detect less fluorophore molecules in the spot. 5000 2013-11-21T22:10:39Z 2012 conferenceObject BASSANI, Thiago et al. Fluorescence-based lab-on-chip spot design for improved signal detection. In: ANNUAL INTERNATIONALCONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, 34., 2012, San Diego. Anais eletrônicos… San Diego, 2012. Disponível em: <http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6345988>. Acesso em: 16 jul. 2013. 978-1-4244-4120-4 1557-170X http://repositorio.utfpr.edu.br/jspui/handle/1/655 http://dx.doi.org/10.1109/EMBC.2012.6345988 eng Annual International Conference of the IEEE Engineering in Medicine and Biology Society http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6345988&tag=1 application/pdf Curitiba |
institution |
Universidade Tecnológica Federal do Paraná |
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RIUT |
language |
Inglês |
topic |
Fluorescência Imunofluorescência Óptica Fluorescence Immunofluorescence Optics |
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Fluorescência Imunofluorescência Óptica Fluorescence Immunofluorescence Optics Bassani, Thiago Dias, Philipe Branco, Gilberto Silva, Wilson José da Vieira Neto, Hugo Schneider, Fábio Kurt Fluorescence-based lab-on-chip spot design for improved signal detection |
description |
This work presents a new perspective for fluorescence signal detection using specific optics on Lab-on-Chip devices. An apparatus was designed and implemented in order to assess the performance of a fluorescence technique using different detection spot configurations, using the chip itself as a waveguide for illumination. The experiments conducted investigate the influence of the dimensions – diameter and height – of the spot on the amplitude of the detection output signal. Results show that the configuration of optical interfaces must be considered in order to improve detection output, or to be able to detect less fluorophore molecules in the spot. |
format |
Trabalho Apresentado em Evento |
author |
Bassani, Thiago Dias, Philipe Branco, Gilberto Silva, Wilson José da Vieira Neto, Hugo Schneider, Fábio Kurt |
author_sort |
Bassani, Thiago |
title |
Fluorescence-based lab-on-chip spot design for improved signal detection |
title_short |
Fluorescence-based lab-on-chip spot design for improved signal detection |
title_full |
Fluorescence-based lab-on-chip spot design for improved signal detection |
title_fullStr |
Fluorescence-based lab-on-chip spot design for improved signal detection |
title_full_unstemmed |
Fluorescence-based lab-on-chip spot design for improved signal detection |
title_sort |
fluorescence-based lab-on-chip spot design for improved signal detection |
publisher |
Curitiba |
publishDate |
2013 |
citation |
BASSANI, Thiago et al. Fluorescence-based lab-on-chip spot design for improved signal detection. In: ANNUAL INTERNATIONALCONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, 34., 2012, San Diego. Anais eletrônicos… San Diego, 2012. Disponível em: <http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6345988>. Acesso em: 16 jul. 2013. 978-1-4244-4120-4 1557-170X |
url |
http://repositorio.utfpr.edu.br/jspui/handle/1/655 http://dx.doi.org/10.1109/EMBC.2012.6345988 |
_version_ |
1805452878620393472 |
score |
10,814766 |