Atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico
The aim of this study was to analyze the influence of different concentrations of crude ethanol extracts (EET) and dichloromethanic (EDC) of antimalarial medicinal species Bidens pilosa L. (beggartick), Phyllanthus niruri L. (shatter stone), Petiveria alliacea L. (guinea) and Senna hirsuta L. (sickl...
| Autor principal: | Ferrari, Ana Paula |
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| Formato: | Dissertação |
| Idioma: | Português |
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Universidade Tecnológica Federal do Paraná
2013
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riut-1-6752015-03-07T06:12:22Z Atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico Ferrari, Ana Paula Marchese, José Abramo Carpes, Solange Teresinha Alelopatia Agentes alelopáticos Plantas medicinais Allelopathy Allelopathic agents Medicinal plants The aim of this study was to analyze the influence of different concentrations of crude ethanol extracts (EET) and dichloromethanic (EDC) of antimalarial medicinal species Bidens pilosa L. (beggartick), Phyllanthus niruri L. (shatter stone), Petiveria alliacea L. (guinea) and Senna hirsuta L. (sicklepod) on germination (GER), mean germination time (TMG), mean speed of germination (VMG), radicle (RAD) and hypocotyls (HIP) of Lactuca sativa L. (lettuce) and Ipomoea grandifolia (Dammer) O'Donel (rope-glory). The experimental design was completely randomized, distributed in a factorial (species antimalarial vs. extractor vs. concentration). Bioassays were performed with four replicates of 25 seeds for germination tests, and 10 pre-germinated seeds for the growth bioassays. Germinated seeds were counted daily for obtaining the TMG and VMG while GER, DAR and HIP were measured after 144 hours of application of the extracts. In parallel to the study of allelopathic activity aimed also to examine the antioxidant and antimicrobial activities of EET and EDC of four malarial species used in bioassays, as well as the species Quassia amara L. (quássia amarga). Analyses antioxidants were performed through three different methodologies, namely: scavenging of ABTS•+, scavenging DPPH•, both performed with EET and EDC, and antioxidant power reduction iron (FRAP), performed with EET, as well as the content of total phenolic compounds (TCF). The EET were compared to commercial antioxidant BHT (butylated hydroxytoluene), BHA (butylated hydroxyanisole) and α-tocopherol. The results of bioassays of extracts of plants antimalarial and their respective concentrations showed inhibitory effects, and in some cases stimulatory, different magnitudes of the processes of germination and/or growth of the target species. Increasing the concentration of EET and EDC of P. alliacea and EET of S. hirsuta, did not show significant results for variables TMG, VMG and GER of corda-de-viola, and the EDC of P. alliacea also did not influence RAD HIP. The EET of B. Pilosa showed excellent results compared to inhibition of RAD and HIP lettuce and GER, TMG, VMG and RAD rope-glory, suggesting the use of this extract for future work of purification, isolation and identification of bioactive substances. In all methods of antioxidant activity analyzed, the EET of Q. amara shown as the most effective treatment, followed by EET of P. niruri, which are analyzed in High Performance Liquid Chromatography Reverse-Phase (HPLC-RP), indicating the presence of rutin, myricetin, quercetin and gallic acid for both species, these compounds being recognized for its antioxidant properties. Analyses were performed using antimicrobial minimum inhibitory concentration (MIC) of the plant extracts with concentrations between 5.0 and 0.88 mg mL-1 in 96-well microplates inoculated with Baccilus cereus ATCC 11.778. The EET of Q. amara, P. niruri and S. hirsuta and EDC of B. pilosa showed minimal inhibitory concentration (MIC) = ≤ 0.088 mg mL-1. Thus, the data presented in this study indicate the potential use of the EET of B. pilosa and EET of Q. amara as sources of molecules having herbicidal activity, antioxidants and antimicrobial properties, and these extracts are important sources for the search of molecules that can be used as a prototype for the development of new products in the agrochemical industries, food and/or pharmaceuticals, requiring future studies on the extraction, purification, isolation and identification of bioactive compounds and their mechanisms of action. CAPES O objetivo desse trabalho foi analisar a influência de diferentes concentrações dos extratos brutos etanólicos (EET) e diclorometânicos (EDC) das espécies medicinais antimaláricas Bidens pilosa L. (picão-preto), Phyllanthus niruri L. (quebra-pedra), Petiveria alliacea L. (guiné) e Senna hirsuta L. (fedegoso), sobre a germinabilidade (GER), tempo médio de germinação (TMG), velocidade média de germinação (VMG), comprimento de radícula (RAD) e comprimento de hipocótilo (HIP) de Lactuca sativa L. (alface) e Ipomoea grandifolia (Dammer) O´Donel (corda-de-viola). O delineamento utilizado foi inteiramente casualizado, distribuído em um trifatorial (espécie antimalárica vs. extrator vs. concentração). Os bioensaios foram realizados com quatro repetições de 25 sementes, para os testes germinativos, e 10 sementes pré-germinadas, para os bioensaios de crescimento. As sementes germinadas foram contadas diariamente, para a obtenção do TMG e VMG, enquanto a GER, RAD e HIP foram mensurados após 144 horas da aplicação dos extratos. Paralelamente ao estudo de atividade alelopática, objetivou-se também analisar a atividade antioxidante e antimicrobiana dos EET e EDC das quatro espécies antimaláricas utilizadas nos bioensaios, assim como, da espécie Quassia amara L. (quássiaamarga). As análises antioxidantes foram realizadas através de três metodologias distintas, sendo elas: sequestro do radical ABTS•+ e sequestro do radical DPPH•, ambas realizadas com os EET e EDC, e poder antioxidante de redução do Ferro (FRAP), realizada apenas com os EET, assim como o teor de compostos fenólicos totais (TCF). Os EET foram comparados aos antioxidantes comerciais BHT (butil hidroxitolueno), BHA (butil hidroxianisol) e α-tocoferol. Os resultados dos bioensaios dos extratos brutos das plantas antimaláricas, e suas respectivas concentrações, demonstraram efeitos inibitórios, e em alguns casos estimulatórios, de magnitudes diferentes sobre os processos de germinação e/ou crescimento das espécies alvo. O aumento das concentrações dos EET e EDC de P. alliacea e do EET de S. hirsuta, não afetaram o TMG, VMG e GER de corda-de-viola, sendo que o EDC de P. alliacea também não influenciou sobre RAD e HIP da mesma, enquanto o EET de B. Pilosa apresentou ótimos resultados de inibição do RAD e HIP de alface e GER, TMG, VMG, RAD e HIP de corda-de-viola. Em todas as metodologias de atividade antioxidante analisadas, o EET de Q. amara demonstrou-se como o tratamento mais eficaz, seguido do EET de P. niruri, sendo estes, analisados em Cromatografia Líquida de Alta Eficiência de Fase-Reversa (CLAE-FR), evidenciando a presença de rutina, miricetina, quercetina e ácido gálico para ambas as espécies, sendo estes compostos, reconhecidos por suas propriedades antioxidantes. As análises antimicrobianas foram realizadas através da Concentração Inibitória Mínima (CIM) dos extratos das plantas, com concentrações entre 5,0 e 0,88 mg mL-1, em microplacas de 96 poços inoculadas com Baccilus cereus ATCC 11.778. Os EET de Q. amara, P. niruri e S. hirsuta e o EDC de B. pilosa apresentaram concentração inibitória mínima (MIC) = ≤0,088 mg mL-1. Dessa forma, os dados apresentados nesse estudo indicam, em especial, um potencial uso do EET de B. pilosa e do EET de Q. amara como fontes de moléculas com atividade herbicida, antioxidantes e antimicrobianas, sendo estes extratos, importantes fontes para a busca de moléculas que possam ser utilizadas como protótipos para o desenvolvimento de novos produtos nas indústrias de agroquímicos, alimentícia e /ou farmacêutica, requerendo estudos futuros quanto a extração, purificação, isolamento e identificação dos compostos bioativos e seus respectivos mecanismos de ação. 2013-12-02T13:06:46Z 2013-12-02T13:06:46Z 2013-07-30 masterThesis FERRARI, Ana Paula. Atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico. 2013. 115 f. Dissertação (Mestrado em Agronomia) - Universidade Tecnológica Federal do Paraná, Pato Branco, 2013. http://repositorio.utfpr.edu.br/jspui/handle/1/675 por application/pdf Universidade Tecnológica Federal do Paraná Pato Branco Programa de Pós-Graduação em Agronomia |
| institution |
Universidade Tecnológica Federal do Paraná |
| collection |
RIUT |
| language |
Português |
| topic |
Alelopatia Agentes alelopáticos Plantas medicinais Allelopathy Allelopathic agents Medicinal plants |
| spellingShingle |
Alelopatia Agentes alelopáticos Plantas medicinais Allelopathy Allelopathic agents Medicinal plants Ferrari, Ana Paula Atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico |
| description |
The aim of this study was to analyze the influence of different concentrations of crude ethanol extracts (EET) and dichloromethanic (EDC) of antimalarial medicinal species Bidens pilosa L. (beggartick), Phyllanthus niruri L. (shatter stone), Petiveria alliacea L. (guinea) and Senna hirsuta L. (sicklepod) on germination (GER), mean germination time (TMG), mean speed of germination (VMG), radicle (RAD) and
hypocotyls (HIP) of Lactuca sativa L. (lettuce) and Ipomoea grandifolia (Dammer)
O'Donel (rope-glory). The experimental design was completely randomized, distributed in a factorial (species antimalarial vs. extractor vs. concentration). Bioassays were performed with four replicates of 25 seeds for germination tests, and 10 pre-germinated seeds for the growth bioassays. Germinated seeds were counted daily for obtaining the TMG and VMG while GER, DAR and HIP were measured after 144 hours of application of the extracts. In parallel to the study of allelopathic activity aimed also to examine the antioxidant and antimicrobial activities of EET and EDC of four malarial species used in bioassays, as well as the species Quassia amara L. (quássia amarga). Analyses antioxidants were performed through three different methodologies, namely: scavenging of ABTS•+, scavenging DPPH•, both performed
with EET and EDC, and antioxidant power reduction iron (FRAP), performed with
EET, as well as the content of total phenolic compounds (TCF). The EET were
compared to commercial antioxidant BHT (butylated hydroxytoluene), BHA (butylated
hydroxyanisole) and α-tocopherol. The results of bioassays of extracts of plants
antimalarial and their respective concentrations showed inhibitory effects, and in some cases stimulatory, different magnitudes of the processes of germination and/or growth of the target species. Increasing the concentration of EET and EDC of P. alliacea and EET of S. hirsuta, did not show significant results for variables TMG, VMG and GER of corda-de-viola, and the EDC of P. alliacea also did not influence
RAD HIP. The EET of B. Pilosa showed excellent results compared to inhibition of
RAD and HIP lettuce and GER, TMG, VMG and RAD rope-glory, suggesting the use
of this extract for future work of purification, isolation and identification of bioactive substances. In all methods of antioxidant activity analyzed, the EET of Q. amara shown as the most effective treatment, followed by EET of P. niruri, which are
analyzed in High Performance Liquid Chromatography Reverse-Phase (HPLC-RP),
indicating the presence of rutin, myricetin, quercetin and gallic acid for both species,
these compounds being recognized for its antioxidant properties. Analyses were
performed using antimicrobial minimum inhibitory concentration (MIC) of the plant
extracts with concentrations between 5.0 and 0.88 mg mL-1 in 96-well microplates
inoculated with Baccilus cereus ATCC 11.778. The EET of Q. amara, P. niruri and S.
hirsuta and EDC of B. pilosa showed minimal inhibitory concentration (MIC) = ≤
0.088 mg mL-1. Thus, the data presented in this study indicate the potential use of the
EET of B. pilosa and EET of Q. amara as sources of molecules having herbicidal
activity, antioxidants and antimicrobial properties, and these extracts are important
sources for the search of molecules that can be used as a prototype for the
development of new products in the agrochemical industries, food and/or
pharmaceuticals, requiring future studies on the extraction, purification, isolation and
identification of bioactive compounds and their mechanisms of action. |
| format |
Dissertação |
| author |
Ferrari, Ana Paula |
| author_sort |
Ferrari, Ana Paula |
| title |
Atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico |
| title_short |
Atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico |
| title_full |
Atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico |
| title_fullStr |
Atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico |
| title_full_unstemmed |
Atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico |
| title_sort |
atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico |
| publisher |
Universidade Tecnológica Federal do Paraná |
| publishDate |
2013 |
| citation |
FERRARI, Ana Paula. Atividade alelopática, antioxidante e antimicrobiana de plantas com uso popular antimalárico. 2013. 115 f. Dissertação (Mestrado em Agronomia) - Universidade Tecnológica Federal do Paraná, Pato Branco, 2013. |
| url |
http://repositorio.utfpr.edu.br/jspui/handle/1/675 |
| _version_ |
1805322841172738048 |
| score |
10,814766 |