Extrato etanólico na alimentação do pacu Piaractus mesopotamicus (Holmberg, 1887): efeitos sobre o desempenho e morfologia intestinal
The objective of this study was to evaluate the performance of fish and morphological alterations of the intestinal epithelium caused by the dietary inclusion of propolis ethanolic extract (EEP) in increasing concentrations (0.0, 1.5, 3.0 and 4.5%), supplied to fish for 60 days. For this purpose, 40...
| Autor principal: | Ponciano Junior, Valfrides Felix |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2020
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/11356 |
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| Resumo: |
The objective of this study was to evaluate the performance of fish and morphological alterations of the intestinal epithelium caused by the dietary inclusion of propolis ethanolic extract (EEP) in increasing concentrations (0.0, 1.5, 3.0 and 4.5%), supplied to fish for 60 days. For this purpose, 40 pacus (208 ± 35.75g) were used in 4 250 L tanks in a system of water recirculation and forced aeration, and each fish being considered as a repetition (n = 10). The fish were housed in homogeneous lots of 10 fish per box, with two meals daily (9hrs-17hrs) until apparent satiety; At the end of 60 days, they were submitted to a 48-hour fasting to decrease the fecal contents of the intestinal tract, and afterwards, were sedated for final biometrics and calculation of the performance variables (weight gain, food consumption and specific growth rate). For the histological analysis, were euthanized (benzocaine anesthetic overdose and posterior medullary section) 4 fish per treatment (n = 4) and a fragment of the previous portion of the intestine was removed for measurement (villus height and thickness of the muscular layer of the intestine). The sections were analyzed under light microscopy using software (ZEN, ZEN 2012 Bluee Edition, Cral Zeiss Microscopy [marca registrada]), and the results were submitted to analyzes of variance at the 5% probability level and when the result is significant, apply Tukey’s test. The inclusion in the concentration of 1.5% of ethanolic propolis extract in the pacus diet has been demonstrated a greater weight gain and specific growth rate in relation to the other treatments. The height of the villousness was not affected by the treatments tested. On the other way, the thickness of the muscular layer was higher in the fish treated with 1.5% of EEP, when compared to the fish that ingested 0,0, 3,0 and 4,5% of EEP in the diet. So, under the conditions that the experiment was carried out, the EEP proved to be efficient as a growth promoter and caused morphological changes in the thickness of the muscular layer of the pacus intestine. |
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