Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera
The Moringa oleifera (Moringacea) is a plant originally from India that has been attracting the interest of researchers and scientists, because of its biological capacity and its proven medicinal qualities, it has already been used to combat high blood pressure, tumors, arthritis and also as a food...
| Autor principal: | Rizzi, Fabio Ricardo |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2020
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/15296 |
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| Resumo: |
The Moringa oleifera (Moringacea) is a plant originally from India that has been attracting the interest of researchers and scientists, because of its biological capacity and its proven medicinal qualities, it has already been used to combat high blood pressure, tumors, arthritis and also as a food reinforcement. Such medicinal properties are attributed to its chemical composition, which presents phenolic compounds, compounds with antioxidant activity and large amounts of vitamins and minerals. Taking into account these properties, it is essential to determine a suitable extraction method that seeks to maximize the yields of compounds present in the plant extract, provided that it is a method that has a good applicability and to that it is effective, economically feasible and likely to be extended to larger scales. To determine the appropriate extraction method, there is the need for scientific basis provided by statistical methods, so a 23 factorial design was performed, in order to evaluate the influence of the independent variables (temperature, ethanol solvent concentration and time extraction) over the dependent variables (responses) that match the antioxidant activity by radical DPPH• (2,2-diphenyl-1-picrylhydrazyl) sequestering methods, radical cation ABTS•+ (2,2-azinobis-[benzotiazolin-3-ethyl-6-sulphonic-acid]) sequestering, FRAP (Ferric Reducing Antioxidant Power) and phenolic content by Folin-Ciocalteu method. To assess whether the influence of independent variables on the responses were statistically significant, the STATISTICA software was used. The results have shown that the experiment 9, which corresponds to the center point (0) of the independent variables was the one who presented better responses for the evaluation of antioxidant activity for the radical ABTS•+,FRAP and total phenolic. For the evaluation of the antioxidant capacity by DPPH• method, the experiment 4 corresponding to top level solvent (+1), higher temperature level (+1) and the lower level of time (-1) has shown the best answer. Statistically, for the ABTS and FRAP (Ferric Reducing Antioxidant Power) antioxidant evaluation methods, the temperature variable and the interaction between temperature and time had a significant influence on the response. For the DPPH response, the variables solvent concentration, temperature, time and interaction between solvent concentration and time were significant. For the total phenolics determination method, the variables time, temperature and solvent/temperature and temperature/time interactions had a significant influence on the responses. Curvature was significant for all responses, this implies evidence that quadratic terms must be determined. The solution is to increase factorial planning with four axial races, resulting in complete planning. |
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