Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera
The Moringa oleifera (Moringacea) is a plant originally from India that has been attracting the interest of researchers and scientists, because of its biological capacity and its proven medicinal qualities, it has already been used to combat high blood pressure, tumors, arthritis and also as a food...
| Autor principal: | Rizzi, Fabio Ricardo |
|---|---|
| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
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Universidade Tecnológica Federal do Paraná
2020
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riut-1-152962020-11-18T19:44:04Z Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera Optimization of bioactive compounds extraction process from the Moringa oleifera leaves Rizzi, Fabio Ricardo Oldoni, Tatiane Luiza Cadorin Oldoni, Tatiane Luiza Cadorin Lima, Vanderlei Aparecido de Calegari, Matheus Augusto Fenóis Antioxidantes Compostos bioativos Plantas - Análise Phenols Antioxidants Bioactive compounds Plants - Analysis CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA The Moringa oleifera (Moringacea) is a plant originally from India that has been attracting the interest of researchers and scientists, because of its biological capacity and its proven medicinal qualities, it has already been used to combat high blood pressure, tumors, arthritis and also as a food reinforcement. Such medicinal properties are attributed to its chemical composition, which presents phenolic compounds, compounds with antioxidant activity and large amounts of vitamins and minerals. Taking into account these properties, it is essential to determine a suitable extraction method that seeks to maximize the yields of compounds present in the plant extract, provided that it is a method that has a good applicability and to that it is effective, economically feasible and likely to be extended to larger scales. To determine the appropriate extraction method, there is the need for scientific basis provided by statistical methods, so a 23 factorial design was performed, in order to evaluate the influence of the independent variables (temperature, ethanol solvent concentration and time extraction) over the dependent variables (responses) that match the antioxidant activity by radical DPPH• (2,2-diphenyl-1-picrylhydrazyl) sequestering methods, radical cation ABTS•+ (2,2-azinobis-[benzotiazolin-3-ethyl-6-sulphonic-acid]) sequestering, FRAP (Ferric Reducing Antioxidant Power) and phenolic content by Folin-Ciocalteu method. To assess whether the influence of independent variables on the responses were statistically significant, the STATISTICA software was used. The results have shown that the experiment 9, which corresponds to the center point (0) of the independent variables was the one who presented better responses for the evaluation of antioxidant activity for the radical ABTS•+,FRAP and total phenolic. For the evaluation of the antioxidant capacity by DPPH• method, the experiment 4 corresponding to top level solvent (+1), higher temperature level (+1) and the lower level of time (-1) has shown the best answer. Statistically, for the ABTS and FRAP (Ferric Reducing Antioxidant Power) antioxidant evaluation methods, the temperature variable and the interaction between temperature and time had a significant influence on the response. For the DPPH response, the variables solvent concentration, temperature, time and interaction between solvent concentration and time were significant. For the total phenolics determination method, the variables time, temperature and solvent/temperature and temperature/time interactions had a significant influence on the responses. Curvature was significant for all responses, this implies evidence that quadratic terms must be determined. The solution is to increase factorial planning with four axial races, resulting in complete planning. A Moringa oleifera (Moringacea) é uma planta originária da Índia que vem despertando interesse de pesquisadores e cientistas, devido sua capacidade biológica e suas comprovadas propriedades medicinais, já sendo usada no combate a hipertensão, tumores, artrite e como reforço alimentar. Tais propriedades medicinais são atribuídas à sua composição química que apresenta compostos fenólicos, compostos com atividade antioxidante e grandes quantidades de vitaminas e sais minerais. Levando em consideração essas propriedades, torna-se indispensável determinar um método de extração adequado que busque maximizar os teores de compostos presentes no extrato da planta, sendo um método que possua uma boa aplicabilidade e que seja eficaz, com viabilidade econômica e passível de ser estendido a maiores escalas. Para determinar o método de extração adequado, destaca-se a necessidade de bases científicas fornecidas por metodologias estatísticas, deste modo foi realizado um planejamento fatorial 23, com o intuito de avaliar as influências das variáveis independentes (temperatura, concentração do solvente etanol e tempo de extração) em relação as variáveis dependentes (respostas) que correspondem a atividade antioxidante pelos métodos de sequestro do radical DPPH• (2,2-difenil-1-picrilhidrazil), sequestro do cátion radical ABTS·+ (2,2-azinobis-[3-etil-benzotiazolin-6-ácido sulfônico]), FRAP (Ferric Reducing Antioxidant Power) e o teor de compostos fenólicos pelo método de Folin-Ciocalteau. Para avaliar se as influências das variáveis independentes sobre as respostas foram significativas estatisticamente foi utilizado o software STATISTICA. Os resultados mostram que o experimento 9 que corresponde ao ponto central (0) das variáveis independentes foi o que apresentou melhores respostas para a avaliação da atividade antioxidante para o método de sequestro do radical ABTS•+, FRAP e teor de fenólicos totais. Para a avaliação da capacidade antioxidante pelo método de DPPH•, o experimento 4 que corresponde ao nível superior de solvente (+1), nível superior de temperatura (+1) e o nível inferior de tempo (-1) foi o que apresentou melhor resposta. Estatisticamente, para os métodos de avaliação da capacidade antioxidante ABTS e FRAP (Ferric Reducing Antioxidant Power), a variável temperatura e a interação entre temperatura e tempo tiveram influência significativa na resposta. Para a resposta DPPH, as variáveis concentração do solvente, temperatura, tempo e a interação entre concentração do solvente e tempo foram significativas. Para o método de determinação de fenólicos totais, as variáveis tempo, temperatura e as interações concentração do solvente/temperatura e temperatura/tempo tiveram influência significativa nas respostas. A curvatura foi significativa para todas as respostas, isso implica na evidência de que termos quadráticos devem ser determinados. A solução é aumentar o planejamento fatorial com quatro corridas axiais, resultando em um planejamento completo. 2020-11-18T19:44:04Z 2020-11-18T19:44:04Z 2016-11-11 bachelorThesis RIZZI, Fábio Ricardo. Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera. 2016. 79 f. Trabalho de Conclusão de Curso (Graduação) - Universidade Tecnológica Federal do Paraná, Pato Branco, 2016. http://repositorio.utfpr.edu.br/jspui/handle/1/15296 por openAccess application/pdf Universidade Tecnológica Federal do Paraná Pato Branco Brasil Departamento Acadêmico de Química Química UTFPR |
| institution |
Universidade Tecnológica Federal do Paraná |
| collection |
RIUT |
| language |
Português |
| topic |
Fenóis Antioxidantes Compostos bioativos Plantas - Análise Phenols Antioxidants Bioactive compounds Plants - Analysis CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
| spellingShingle |
Fenóis Antioxidantes Compostos bioativos Plantas - Análise Phenols Antioxidants Bioactive compounds Plants - Analysis CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA Rizzi, Fabio Ricardo Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera |
| description |
The Moringa oleifera (Moringacea) is a plant originally from India that has been attracting the interest of researchers and scientists, because of its biological capacity and its proven medicinal qualities, it has already been used to combat high blood pressure, tumors, arthritis and also as a food reinforcement. Such medicinal properties are attributed to its chemical composition, which presents phenolic compounds, compounds with antioxidant activity and large amounts of vitamins and minerals. Taking into account these properties, it is essential to determine a suitable extraction method that seeks to maximize the yields of compounds present in the plant extract, provided that it is a method that has a good applicability and to that it is effective, economically feasible and likely to be extended to larger scales. To determine the appropriate extraction method, there is the need for scientific basis provided by statistical methods, so a 23 factorial design was performed, in order to evaluate the influence of the independent variables (temperature, ethanol solvent concentration and time extraction) over the dependent variables (responses) that match the antioxidant activity by radical DPPH• (2,2-diphenyl-1-picrylhydrazyl) sequestering methods, radical cation ABTS•+ (2,2-azinobis-[benzotiazolin-3-ethyl-6-sulphonic-acid]) sequestering, FRAP (Ferric Reducing Antioxidant Power) and phenolic content by Folin-Ciocalteu method. To assess whether the influence of independent variables on the responses were statistically significant, the STATISTICA software was used. The results have shown that the experiment 9, which corresponds to the center point (0) of the independent variables was the one who presented better responses for the evaluation of antioxidant activity for the radical ABTS•+,FRAP and total phenolic. For the evaluation of the antioxidant capacity by DPPH• method, the experiment 4 corresponding to top level solvent (+1), higher temperature level (+1) and the lower level of time (-1) has shown the best answer. Statistically, for the ABTS and FRAP (Ferric Reducing Antioxidant Power) antioxidant evaluation methods, the temperature variable and the interaction between temperature and time had a significant influence on the response. For the DPPH response, the variables solvent concentration, temperature, time and interaction between solvent concentration and time were significant. For the total phenolics determination method, the variables time, temperature and solvent/temperature and temperature/time interactions had a significant influence on the responses. Curvature was significant for all responses, this implies evidence that quadratic terms must be determined. The solution is to increase factorial planning with four axial races, resulting in complete planning. |
| format |
Trabalho de Conclusão de Curso (Graduação) |
| author |
Rizzi, Fabio Ricardo |
| author_sort |
Rizzi, Fabio Ricardo |
| title |
Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera |
| title_short |
Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera |
| title_full |
Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera |
| title_fullStr |
Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera |
| title_full_unstemmed |
Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera |
| title_sort |
otimização do processo de extração de compostos bioativos a partir das folhas de moringa oleifera |
| publisher |
Universidade Tecnológica Federal do Paraná |
| publishDate |
2020 |
| citation |
RIZZI, Fábio Ricardo. Otimização do processo de extração de compostos bioativos a partir das folhas de Moringa oleifera. 2016. 79 f. Trabalho de Conclusão de Curso (Graduação) - Universidade Tecnológica Federal do Paraná, Pato Branco, 2016. |
| url |
http://repositorio.utfpr.edu.br/jspui/handle/1/15296 |
| _version_ |
1805302589033545728 |
| score |
10,814766 |