Controle de qualidade microbiológico em frigorífico
An unhygienic surface in a productive environment, added to the adhesion capacity of a microorganism, can become a potential source of contamination and lead to the formation of biofilms. These, once formed, are difficult to remove and can proliferate for food contamination. Concern about food safet...
| Autor principal: | Stocco, Claudia Walus |
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| Formato: | Dissertação |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2017
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/2301 |
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| Resumo: |
An unhygienic surface in a productive environment, added to the adhesion capacity of a microorganism, can become a potential source of contamination and lead to the formation of biofilms. These, once formed, are difficult to remove and can proliferate for food contamination. Concern about food safety is a challenge, as related problems can compromise consumer health. The objective of this work is to select potential pathogenic microorganisms producing microbial biofilms present in the industrial processing of a beef cattle. The development of the work is summarized in three phases: interview with the quality coordinator of a refrigerator in the Campos Gerais region; diagnosis of critical points in the quality control of the industrial processing of this refrigerator, through a decision diagram and sample collection during the industrial process through swabs, used in the isolation by microbiology. Then, the genetic profile of the samples was identified through DNA isolation, followed by amplification by Polymerase Chain Reaction with the universal primers rD1 and fD1. The data generated in the first phase indicated the quality control programs in the refrigeration industry under study. The interviewee, responsible for the quality control of the industry, emphasized the use of Good Manufacturing Practices (GMP), Hazard Analysis and Critical Control Points (HACCP), Standard Operating Procedures (PPHO), Pest Monitoring (IPM) And Verification Sheet (FV). From the decision diagram, 25 points were identified for the collection of samples for the identification of pathogenic microorganisms. Among these, ten sample points were isolated by conventional microbiology with EMB culture, indicating contamination of gastrointestinal contents by fecal coliforms. At ten points, not always distinct, there was growth in the SS - Salmonella Shigella culture, indicating contamination during slaughter from the handling of the meat by the employees, since they may be healthy carriers of pathogenic microorganisms. For genotypic identification of the sequenced samples, the results reached the species level, being Escherichia, Proteus, Hafnia and Bacillus, all belonging to the group of Enterobacteria, except for Bacillus. It was verified through the genotypic identification, related to the sample collection sites in the flowchart, that there is cross contamination in the productive environment of the present refrigerator, in most of the points, related to the manipulator. |
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