Encapsulamento, caracterização e avaliação da atividade antioxidante e antimicrobiana do extrato aquoso de Baccharis trimera (Less.) DC para o controle de patógenos entéricos de suínos
The Baccharis trimera plant is popularly used as a herbal product, its application is mainly aimed at treating digestive problems, but it also has important anti-inflammatory and antimicrobial effects. The objective of the present work was to analyze in vitro the antioxidant activity and antimicrobi...
| Autor principal: | Brusamarello, Micheli Negri |
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| Formato: | Dissertação |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2022
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/28964 |
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| Resumo: |
The Baccharis trimera plant is popularly used as a herbal product, its application is mainly aimed at treating digestive problems, but it also has important anti-inflammatory and antimicrobial effects. The objective of the present work was to analyze in vitro the antioxidant activity and antimicrobial potential of the extract of B. trimera in the control of enteric pathogens in swine and its viability as a gastroprotective agent. The species was collected in a rural area of the city of Verê-PR and the aqueous extract of the aerial part was prepared in the laboratory of the Universidade Tecnológica Federal do Paraná (UTFPR). Tests were carried out to quantify total phenolics, antioxidant activity by the methods of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), activity antimicrobial activity by plate microdilution test, spot-test and inhibition curve, in addition to chemical characterization by High Performance Liquid Chromatography (HPLC). After encapsulation with sodium alginate, the product was subjected to scanning electron microscopy micrography, evaluation of total phenolics, solubility, encapsulation efficiency, diameter and release kinetics of the encapsulate. The results of the total phenolics and antioxidant activity assays showed important free radical scavenging activity, while the assays evaluating the antimicrobial activity showed an important bacteriostatic effect on the clinical isolates of swine and ATTCs (American Type Culture Collection) bacteria tested. HPLC of the aqueous extract revealed the presence of chlorogenic acid in higher concentration, ferulic acid, caffeic acid and cinnamic acid, which are possibly associated with important antioxidant and antimicrobial activity. The aqueous extract encapsulate presented 102 ± 0.40 mg gallic acid equivalent, 25.3% water solubility, encapsulation efficiency and 73% extract load. Electron microscopy revealed capsules with an irregular and rough surface, not showing uniform dimensions, but the entrapment of the encapsulated content was satisfactory. The encapsulate diameter ranged from 1.81-1.46 mm and the surface area from 37.18 to 47.74 cm2/cm3 between the two evaluated samples. The in vitro release profile of the encapsulated extract was investigated in simulated gastric fluid (pH 3.0) and simulated intestinal fluid (pH 7.4) and revealed slow and sustained release at alkaline pH for 48h. The results indicate an important biological potential in the use of the aqueous extract of B. trimera as a natural source for the production of future commercial drugs. |
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