Validação de marcadores do tipo SSRs identificados em regiões de QTLs relacionados com a resistência à antracnose no feijoeiro
In order to have increasingly better and bigger production, plants are submitted to selections, crossbreds and DNA's modifications, being these the main vegetal improving resources. About plants' selection, nowadays there are techniques which allow to realize this selection only from their...
| Autor principal: | Sartor, Klaiton Luis Cagnini |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Agronomia
2022
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/30046 |
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| Resumo: |
In order to have increasingly better and bigger production, plants are submitted to selections, crossbreds and DNA's modifications, being these the main vegetal improving resources. About plants' selection, nowadays there are techniques which allow to realize this selection only from their DNA, without the necessity of expressing the individual's phonotype. One of these techniques is the marker-assisted selection (MAS). The called microsatellites markers or Simple Sequence Repeat (SSRS) are advocated by many researchers as the ones which are more competent and applicable to several species. One of the applicabilities of such markers is the selection of plants resistant to pathogens, for instance, the resistance of beans (Phaseolus vulgaris L.) to anthracnose (Colletotrichum lindemuthianum). The aim of this resource was to validate SSRS molecular markers previously identified in regions of QTLs (Quantitative Trait Loci) on bean plants to analyze if they are actually related to anthracnose resistance. This survey started by planting 16 beans' genotypes and still on its vegetative stage their leaves were collected in order to extract each genotype. Having the AND in vitro, 9 molecular markers were amplified through PCR (Polymerase Chain Reaction) and after that the bands amplified on agarose gel were compared with two parental bands, CAL-143 and IAC-UNA, being one susceptible and the other resistant to anthracnose, respectively. From the field data, where were evaluated the 16 genotypes' resistance to anthracnose, it was made a comparative with the amplifications in agarose gel. Five valid markers can be pointed: SSR-IAC284, SSR-IAC65, SSR-IAC239, SSR-IAC245 e SSR-IAC268. However, more tests need to be done for the other markers intending to prove if they are or are not really related to anthracnose resistance. This resource result allows these 5 markers to be used in future plants assisted selections, to make the improver work quicker and more efficient. |
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