Encapsulamento de extrato de goji berry, com atividade antiproliferativa, via spray dryer e cloreto de colina
In recent decades, research involving functional foods has gained prominence for its potential to improve quality of life and prevent chronic diseases such as cancer. The Goji Berry (Lycium barbarum) is one of these superfoods, due to its composition rich in carotenoids, phenolic compounds and polys...
| Autor principal: | Fragoso, Ágatha Giovanna |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2023
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/30830 |
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| Resumo: |
In recent decades, research involving functional foods has gained prominence for its potential to improve quality of life and prevent chronic diseases such as cancer. The Goji Berry (Lycium barbarum) is one of these superfoods, due to its composition rich in carotenoids, phenolic compounds and polysaccharides. Since the encapsulation of bioactive compounds becomes a way to preserve and improve the characteristics of the ingredients, the objective of the study was to produce microcapsules with goji berry extract that present biological/antitumor activity, using spray drying and the ionic liquids as reticulant. The evaluation of the cytotoxic/antiproliferative activity of the pure extract of Goji Berry (5, 10, 50, 100, 200, 300, 400, 500 and 1000 μg mL-1), produced by means of a shaker and ultrasound, was performed by the test of MTT in normal (K2) and renal (786-0) and mammary (MCF-7) tumor cells, for periods of 24, 48 and 72 hours. The absorbance data obtained indicated cytotoxicity of Goji Berry for renal, especially in higher concentrations and after 48 hours. The production of the microcapsules using spray dryer was successful and the characterization of the particles confirmed the presence of the encapsulated extract and the influence of the ionic liquid on the reticulation of the capsules. When carrying out the release test of the extract in cell culture medium between the times of 3 to 72 hours, the principle of controlled drug release intended based on the cell pH was confirmed. The data show that other studies must be carried out, with different cell lines and extraction methods, different forms of production and characterization of the particles, since the technology used in this research has the potential to be used for medicinal purposes. |
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