Registro fonte: Poly(ε-caprolactone)/zein based scarffolds produced via electrospinning

Poly(ε-caprolactone)/zein based scarffolds produced via electrospinning

Several studies investigated the electrospinnability of poly (ε-caprolactone) blends with polysaccharides and proteins for producing cytocompatible and hydrophilic membranes for tissue engineering purposes. However, there are no reports of electrospun membranes based on poly(ε-caprolactone)/N,N-dime...

ver descrição completa

Autor principal:	Plath, André Mathias Souza
Formato:	Dissertação
Idioma:	Português
Publicado em:	Universidade Tecnológica Federal do Paraná 2020
Assuntos:	Polímeros Polissacarídeos Proteínas Eletrofiação Polymers Polysaccharides Proteins Electrospinning CNPQ::ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA Materiais
Acesso em linha:	http://repositorio.utfpr.edu.br/jspui/handle/1/4756
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!

id	riut-1-4756
recordtype	dspace
spelling	riut-1-47562021-02-12T13:22:26Z Poly(ε-caprolactone)/zein based scarffolds produced via electrospinning Plath, André Mathias Souza Martins, Alessandro Francisco http://lattes.cnpq.br/6465172583349458 Corradini, Elisangela http://lattes.cnpq.br/1145425960313431 Martins, Alessandro Francisco http://lattes.cnpq.br/6465172583349458 Piai, Juliana Francis http://lattes.cnpq.br/4229214524955307 Monteiro, Johny Paulo https://orcid.org/0000-0001-7402-2163 http://lattes.cnpq.br/5157402662010182 Polímeros Polissacarídeos Proteínas Eletrofiação Polymers Polysaccharides Proteins Electrospinning CNPQ::ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA Materiais Several studies investigated the electrospinnability of poly (ε-caprolactone) blends with polysaccharides and proteins for producing cytocompatible and hydrophilic membranes for tissue engineering purposes. However, there are no reports of electrospun membranes based on poly(ε-caprolactone)/N,N-dimethyl chitosan and poly(ε-caprolactone)/zein blends carried out in a 70/30 formic acid/acetic acid mixture (70/30 vol/vol). By adjusting poly(ε-caprolactone)/N,N-dimethyl chitosan and poly(ε-caprolactone)/zein weight ratios, we developed nanofibers with average diameters ranging from 200 to 500 nm. The poly(ε-caprolactone)-based blends are electrospinnable with until 40 wt% N, N-dimethyl chitosan or 50 wt% zein contents. The infrared spectroscopy and X-ray excited photoelectron spectroscopy characterized the materials. Contact angle measurements point that the insertion of N,N-dimethyl chitosan and zein increased the hydrophilicity of the PCL-based membranes. The contact angle decreases from 118º (pure poly (ε-caprolactone) nanofiber) to 76º (8% poly (ε-caprolactone)/ 30 wt% N,N-dimethyl chitosan content) and 73º ( 8% poly(ε-caprolactone)/40 wt% zein content). Cell adhesion and proliferation studies indicate that the poly(εcaprolactone)/zein nanofibers containing 30 and 40 wt% worked as scaffold materials. The membranes promoted cell adhesion, proliferation, and spreading of adipose-derived human stem cells after 7 days of incubation. The results indicate that the poly(ε-caprolactone)/zein electrospun membranes may be applied as scaffolds in tissue engineering purposes. Vários estudos investigaram a eletrofiação de blendas à base de poli(ε-caprolactona) com polissacarídeos e proteínas para produzir materiais citocompatíveis e hidrofílicos para aplicação na área de engenharia de tecidos. No entanto, ainda não há relatos da eletrofiação de blendas poliméricas à base de poli(ε-caprolactona)/N,N-dimetil quitosana e poli(εcaprolactona)/zeína no sistema de solvente ácido fórmico/ácido acético (razão 70/30 vol/vol). Ajustando as proporções em massa de poli(ε-caprolactona)/N,N-dimetil quitosana e poli(εcaprolactona)/zeína, desenvolvemos nanofibras com diâmetros médios entre 200 a 500 nm. As misturas à base de poli(ε-caprolactona) são eletrofiáveis com até 40% em massa de N,Ndimetil-quitosana e até 50% em massa de zeína. A espectroscopia na região do infravermelho, espectroscopia de fotoelétrons excitados por raios X caracterizaram os materiais. Medidas de ângulo de contato indicaram que a inserção da N,N-dimetil quitosana e zeína aumentaram a hidrofilicidade das membranas. O ângulo de contato diminui de 118º (nanofibra de poli(εcaprolactona pura) para 76º (8% poli(ε-caprolactona)/ 30 m% N,N-dimetil quitosana) e 73º (8% poli(ε-caprolactona)/ 40 m% zeína). Estudos de adesão e proliferação celular indicaram que as nanofibras de poli(ε-caprolactona)/zeína contendo 30 e 40 m% de zeína atuaram como materiais scaffolds. As membranas promoveram adesão, proliferação e espalhamento celular de células tronco do tecido adiposo humano em apenas 7 dias de incubação. Os resultados indicam que os materiais poly(ε-caprolactone)/zeína podem ser aplicados no campo de engenharia de tecidos como materiais scaffolds. 2020-03-06T19:41:23Z 2022-03-01 2020-03-06T19:41:23Z 2020-02-17 masterThesis PLATH, André Mathias Souza. Poly(ε-caprolactone)/zein based scaffolds produced via electrospinning. 2020. 56 f. Dissertação (Mestrado em Ciência e Engenharia de Materiais) - Universidade Tecnológica Federal do Paraná, Londrina, 2020. http://repositorio.utfpr.edu.br/jspui/handle/1/4756 por embargoedAccess application/pdf Universidade Tecnológica Federal do Paraná Londrina Brasil Programa de Pós-Graduação em Ciência e Engenharia de Materiais UTFPR
institution	Universidade Tecnológica Federal do Paraná
collection	RIUT
language	Português
topic	Polímeros Polissacarídeos Proteínas Eletrofiação Polymers Polysaccharides Proteins Electrospinning CNPQ::ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA Materiais
spellingShingle	Polímeros Polissacarídeos Proteínas Eletrofiação Polymers Polysaccharides Proteins Electrospinning CNPQ::ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA Materiais Plath, André Mathias Souza Poly(ε-caprolactone)/zein based scarffolds produced via electrospinning
description	Several studies investigated the electrospinnability of poly (ε-caprolactone) blends with polysaccharides and proteins for producing cytocompatible and hydrophilic membranes for tissue engineering purposes. However, there are no reports of electrospun membranes based on poly(ε-caprolactone)/N,N-dimethyl chitosan and poly(ε-caprolactone)/zein blends carried out in a 70/30 formic acid/acetic acid mixture (70/30 vol/vol). By adjusting poly(ε-caprolactone)/N,N-dimethyl chitosan and poly(ε-caprolactone)/zein weight ratios, we developed nanofibers with average diameters ranging from 200 to 500 nm. The poly(ε-caprolactone)-based blends are electrospinnable with until 40 wt% N, N-dimethyl chitosan or 50 wt% zein contents. The infrared spectroscopy and X-ray excited photoelectron spectroscopy characterized the materials. Contact angle measurements point that the insertion of N,N-dimethyl chitosan and zein increased the hydrophilicity of the PCL-based membranes. The contact angle decreases from 118º (pure poly (ε-caprolactone) nanofiber) to 76º (8% poly (ε-caprolactone)/ 30 wt% N,N-dimethyl chitosan content) and 73º ( 8% poly(ε-caprolactone)/40 wt% zein content). Cell adhesion and proliferation studies indicate that the poly(εcaprolactone)/zein nanofibers containing 30 and 40 wt% worked as scaffold materials. The membranes promoted cell adhesion, proliferation, and spreading of adipose-derived human stem cells after 7 days of incubation. The results indicate that the poly(ε-caprolactone)/zein electrospun membranes may be applied as scaffolds in tissue engineering purposes.
format	Dissertação
author	Plath, André Mathias Souza
author_sort	Plath, André Mathias Souza
title	Poly(ε-caprolactone)/zein based scarffolds produced via electrospinning
title_short	Poly(ε-caprolactone)/zein based scarffolds produced via electrospinning
title_full	Poly(ε-caprolactone)/zein based scarffolds produced via electrospinning
title_fullStr	Poly(ε-caprolactone)/zein based scarffolds produced via electrospinning
title_full_unstemmed	Poly(ε-caprolactone)/zein based scarffolds produced via electrospinning
title_sort	poly(ε-caprolactone)/zein based scarffolds produced via electrospinning
publisher	Universidade Tecnológica Federal do Paraná
publishDate	2020
citation	PLATH, André Mathias Souza. Poly(ε-caprolactone)/zein based scaffolds produced via electrospinning. 2020. 56 f. Dissertação (Mestrado em Ciência e Engenharia de Materiais) - Universidade Tecnológica Federal do Paraná, Londrina, 2020.
url	http://repositorio.utfpr.edu.br/jspui/handle/1/4756
_version_	1805318356547403776
score	10,814766

Poly(ε-caprolactone)/zein based scarffolds produced via electrospinning

Registros relacionados