Análise de hormônios em águas residuárias e naturais, derivada da extração com ponteiras descartáveis e detecção por HPLC-FL
Water resources are a means of receiving domestic, industrial and agricultural sewage and, therefore, are the main vehicle for the environmental dispersion of chemical pollutants. For this reason, the issue of the environment, and more specifically of water, remains a current issue at national and i...
| Autor principal: | Cordeiro, Leticia Darlla |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2020
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/6100 |
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| Resumo: |
Water resources are a means of receiving domestic, industrial and agricultural sewage and, therefore, are the main vehicle for the environmental dispersion of chemical pollutants. For this reason, the issue of the environment, and more specifically of water, remains a current issue at national and international level. Urban growth and population concentration in urban centers coupled with precarious sanitation and new consumption habits have contributed to the launching of thousands of substances known as emerging pollutants (rivers, lakes and underground deposits) human beings. Emerging pollutants present very low concentrations in natural aquatic environments, ranging from μg L-1 to ng L-1, in some cases even lower concentration levels have already been analyzed, therefore, it is necessary that the preparation of samples is efficient to concentrate and minimize interference for the determination of emerging contaminants. This project involved a study in which, from the use of disposable tips (DPX), the emergent contaminants were extracted, in this case the female hormones, from wastewater and natural waters. After the extraction procedure, the hormones present in the water samples were analyzed by Fluorescence Coupling High-Performance Liquid Chromatography (HPLC-FL). The results showed the presence of three hormones in the samples, estriol, B-Estradiol and 17-α-Ethinyl estradiol, with concentrations in the µg L-1 range. Extraction using DPX proved to be very efficient despite the relatively new method. It is a single method in which the sorbent contained freely within a tip is thoroughly mixed with the sample solution through the aspiration of air. This dynamic mixture of the solid dispersive-sample phase favors the rapid equilibrium sorption of the analyte. The lower sorbent mass results in lower sample and organic solvent volumes. In addition, DPX extraction can be easily automated along with HPLC-FL detection. |
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