Propriedades antioxidantes e perfil dos compostos fenólicos de cogumelos
Mushrooms have been part of the normal human diet for thousands of years and, in recent times, the amounts consumed have risen greatly, involving a large number of species. Besides the nutritional properties, mushrooms have been demonstrated to possess healthy properties. In this study, three variet...
| Autor principal: | Stafussa, Ana Paula |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2020
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/6772 |
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| Resumo: |
Mushrooms have been part of the normal human diet for thousands of years and, in recent times, the amounts consumed have risen greatly, involving a large number of species. Besides the nutritional properties, mushrooms have been demonstrated to possess healthy properties. In this study, three varieties of mushrooms were evaluated: Champignon, Shimeji and Shiitaki. Absolute methanol was used to obtain the crude extracts of the mushrooms (1:20, w/v). The total phenolic compounds of the mushrooms were determined using the Folin-Ciocalteau method. Flavonoids were estimated using the colorimetric method with aluminium chloride and the pH differential assay was used to quantify the total anthocyanins in the samples.1,1- Diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity and coupled autoxidation of β- carotene and linoleic acid assays were used to obtain the EC50 values of the samples. Reverse-phase high-performance liquid chromatography with diode-array detection (RP-HPLCDAD-UV/Vis) was used for the separation and identification of phenolic compounds in the mushrooms extracts. Among the three mushrooms studied, champignon presented the highest concentration of total phenolic compounds (6.40 ± 0.22 mg GAE/g dry basis) and flavonoids (2.36 ± 0.19 mg CE/g dry basis). Anthocyanins were only detected in the sample of Shimeji (2.54 ± 0.13 mg/100g dry basis). The mushroom with the highest antioxidant potential was shiitaki (EC50 values of 16.83 ± 0.31 μg/mL and 26.46 ± 0.37 μg/mL in the DPPH and in the β-carotene/ linoleic acid assays, respectively). Different phenolic compounds were identified by HPLC analysis in each sample. The highest concentration was found in Champignon, being rutin significantly present. |
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