Desenvolvimento de um método analítico para quantificação de resíduos de carbendazim em sucos de laranjas
Carbedazim is a fungicide used in agriculture to controll a wide range of fungi in fruits, such as citric fruits. The ingestion of Carbedazim in a dose higher than the TLV (threshold limit value) allowed by the legislation provokes infertility, testicular problems, toxicity to organisms present in s...
| Autor principal: | Curti, Suzana Moreira Muniz |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2020
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/6805 |
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| Resumo: |
Carbedazim is a fungicide used in agriculture to controll a wide range of fungi in fruits, such as citric fruits. The ingestion of Carbedazim in a dose higher than the TLV (threshold limit value) allowed by the legislation provokes infertility, testicular problems, toxicity to organisms present in soil, among other consequences. Due to this, the use of Carbedazim is prohibited in several countries. The objective of the present work was to develop an alternative methodology for quantify Carbedazim in orange juice. The standard method in literature used for quantify Carbedazim a complexing reaction followed by a liquid-liquid extraction being the absorbance of final extract measured at =478 nm. The disadvantage of such method is the number of steps, the use of reactants and solvents and the chemical-waste generation. In the developed methodology, Carbendazim were directly added to the orange juice and an UV-Vis spectrum 200 – 850 nm wavelength range was obtained after each addition. The data of spectra (intensity vs wavelength) were further organized in a matrix in which the chemometric method MCR-ALS of multivariate resolution was processed. The profile of Carbedazim spectrum recovered after MCR-ALS treatment matches to the Carbedazim spectrum profile shown in literature. The concentration profile related to Carbedazim was used for building a pseudo-univariate calibration curve. The results obtained through the proposed methodology were compared to the obtained by the standard method. The standard errors associated to the determinations using conventional method (standard one) were higher than those obtained through the UV-Vis combined to the chemometric MCR-ALS. Thus, the methodology developed in the present work is fast, not expensive and can be used in routine labs for quality control in orange juice industries. Beyond this, the new method is in accordance to the Green chemistry principle mainly in minimizing the use of chemical reactants, solvents and lowering the chemical-waste generation. |
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