Extração aquosa de compostos fenólicos presentes em folhas de amoreira preta (Morus nigra L) e encapsulamento em esferas de alginato de cálcio

The black mulberry, belong to the genus Morus, which has secondary metabolites, mainly phenolic compounds. These compounds are known to act in the inhibition of oxidation reactions, having antioxidant properties. They are highly reactive, susceptible to enzyme action, had low solubility and have a h...

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Autor principal: Souza, Crisiane Iozwiak de
Formato: Trabalho de Conclusão de Curso (Graduação)
Idioma: Português
Publicado em: Universidade Tecnológica Federal do Paraná 2020
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Acesso em linha: http://repositorio.utfpr.edu.br/jspui/handle/1/16445
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Resumo: The black mulberry, belong to the genus Morus, which has secondary metabolites, mainly phenolic compounds. These compounds are known to act in the inhibition of oxidation reactions, having antioxidant properties. They are highly reactive, susceptible to enzyme action, had low solubility and have a half-life gastrointestinal system. It is necessary to quickly extract the phenolic compounds and to avoid their degradation before starting to be absorbed by the body. One solution to this is an infusion extraction and followed encapsulation. Encapsulation is a process in which thin films, usually of polymeric materials, are applied to small solid particles, liquids or droplets of gases. This method is used to intercept active components and release them under controlled conditions. This work aims to optimize the process of extracting and encapsulating phenolic compounds from black mulberry leaves. Wet and dried calcium alginate beads were examined and compared in relation to their total phenolic compounds (TPC) and entrapment efficiency based on the effects caused by storage conditions. High encapsulation efficiency values were obtained for the wet (83.5%) and for the dried (92.5%) beads. Drying significantly affected bead size and morphology and proved to be an appropriate method for preservation of encapsulated polyphenols. Release of encapsulated polyphenols in water was measured to analyse the diffusion and kinetic behaviour of the system. Regarding of the stability, analyzed for 28 days, wet calcium alginate beads stored in flasks containing the optimized extract showed significantly higher TPC when compared to the same type of beads stored in water. By the present study it can be verified that the encapsulation technique of Morus nigra L extract in calcium alginate spheres is highly effective in capturing the desired bioactives and delaying their release profile.