Produção de ácido lático a partir do melaço de cana-de-açúcar
Lactic acid is a compound with extensive application in the industry, mainly in the food, chemical, pharmaceutical and textile sectors. As a highlight in recent years, polylactic acid (PLA) can be mentioned, used in the packaging area and for the manufacture of medical items with bio resorption. Due...
| Autor principal: | Grocholski, Luís Felipe |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2020
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/16484 |
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| Resumo: |
Lactic acid is a compound with extensive application in the industry, mainly in the food, chemical, pharmaceutical and textile sectors. As a highlight in recent years, polylactic acid (PLA) can be mentioned, used in the packaging area and for the manufacture of medical items with bio resorption. Due to its applicability and environmental concern, lactic acid produced by fermentation using industrial waste is a process with a lot of focus on research, aiming at viability and productivity. The present work aimed to produce lactic acid through fermentation process using sugar cane molasses 10% (m/v) as a culture medium supplemented with different nitrogen sources: urea 2% (m/v), yeast extract 2% (m/v) and permeated whey 2% (m/v). The culture medium was inoculated with Lactobacillus johnsonii and Lactobacillus helveticus and the condition that presented the best biomass result, considering cost x benefit, was staggered into the reactor. In the reactor the fermentation conditions were: pH 5.7±0.2; temperature 37 °C; agitation at 100 rpm; maintained for 48 h. The processes were monitored through: biomass production; evaluation of the increase in lactic acid concentration using HPLC with wavelength 210 nm, flow of 0.3 mL/min and mobile phase Na2SO4 40mM; evaluation of the consumption of total sugars using the Phenol-Sulfuric method with spectrophotometric readings at 490 nm; viability of using milk permeated whey. It was noticed that after 2 h of fermentation the stability of production was achieved. The process of producing lactic acid with the given conditions proved feasible, achieving a cost reduction of 8 times in relation to the use of yeast extract and producing about 4.04 g/Lh. The product/ substrate yield was 5.13 g/g, demonstrating a small consumption of the substrate for the production of lactic acid. However, for future work it is recommended to reduce fermentation time with removal of samples at shorter intervals. |
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