Efeito estressor do glifosato e paraquate em Saccharomyces cerevisiae
Glyphosate and paraquat are herbicides used in many countries. The disposal of these products and their metabolites lead to the contamination of several environmental compartments, and are often conducted to sewage and water treatment plants. Studies report that exposure to these compounds may lead...
| Autor principal: | Ianoski, Aline Bisinella |
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| Formato: | Dissertação |
| Idioma: | Português |
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Universidade Tecnológica Federal do Paraná
2019
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riut-1-43152019-08-08T06:00:41Z Efeito estressor do glifosato e paraquate em Saccharomyces cerevisiae Stressor effect of glyphosate and paraquat on Saccharomyces cerevisiae Ianoski, Aline Bisinella Haminiuk, Charles Windson Isidoro http://lattes.cnpq.br/3110129398595336 Maciel, Giselle Maria http://lattes.cnpq.br/1525661213489573 Haminiuk, Charles Windson Isidoro Soares, Marlene Alvarez, Thabata Maria Leveduras Herbicidas Radicais livres (Química) Saccharomyces cerevisiae Antioxidantes Enzimas Catalase Estresse oxidativo Tecnologia ambiental Yeast Herbicides Free radicals (Chemistry) Saccharomyces cerevisiae Antioxidants Enzymes Catalase Oxidative stress Green technology CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA::SANEAMENTO AMBIENTAL::QUALIDADE DO AR, DAS AGUAS E DO SOLO Ciências Ambientais Glyphosate and paraquat are herbicides used in many countries. The disposal of these products and their metabolites lead to the contamination of several environmental compartments, and are often conducted to sewage and water treatment plants. Studies report that exposure to these compounds may lead to oxidative stress in organisms. An exaggerated stimulus in the production of free radicals, such as reactive oxygen species (ROS), may be related to more than fifty diseases. One way to monitor the effect of these micropollutants on living things is to employ Saccharomyces cerevisiae as a eukaryotic model. In this context, this yeast was used to evaluate the stressor effect generated by the micropollutants glyphosate and paraquat, both with the analytical standards and with its commercial formulations TROP® and TOCHA®, respectively. Cell survival analyzes were performed at different time periods, from the generation of ROS and the determination of the catalase (CAT) and superoxide dismutase (SOD) enzymes after 2 and 3 hours of exposure to the pesticides. For the cell survival assay the cells from the logarithmic growth phase were treated with the analytical standards glyphosate and paraquat at the concentrations of 50, 100 and 200 μmol L-¹. The concentration that most affected the growth rate of the yeast was 200 μmol L-¹, and this was the concentration applied in the other assays. In the survival trials with the commercial formulations TROP® (glyphosate) and TOCHA® (paraquat) the cell growth rate was drastically affected. There was an increase in the production of ROS during the second and third hour of exposure to almost all the compounds analyzed, except for the paraquat analytical standard in the second hour. CAT production in the second hour increased only to the glyphosate analytical standard, and in the third hour it increased for both the substance and the TROP® commercial formulation. For the other substances studied, there were no significant changes in CAT production. The enzyme SOD increased in the presence of the analytical standards glyphosate and paraquat in the second hour, and in the third hour there was a significant reduction in the enzymatic activity against analytical paraquate. Commercial formulations TROP® and TOCHA® showed no significant difference in the prodution of SOD in relation to the control in any of the analyzed periods. The results obtained show that there is a need to address not only the active ingredients of pesticides, but especially their commercial formulations, as this is how pesticides are made available in the environment. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) O glifosato e o paraquate são herbicidas utilizados em vários países. O descarte destes produtos e seus metabólitos ocasionam a contaminação de diversos compartimentos ambientais, sendo muitas vezes conduzidos para estações de tratamento de esgoto e de água. Estudos relatam que a exposição a estes compostos pode ocasionar estresse oxidativo nos organismos. Um estímulo exagerado na produção de radicais livres, como as espécies reativas de oxigênio (EROs), pode estar relacionado a mais de cinquenta doenças. Uma maneira de verificar o efeito destes micropoluentes nos seres vivos é empregar a Saccharomyces cerevisiae como um modelo eucariótico. Neste contexto, utilizou-se esta levedura para avaliação do efeito estressor gerado pelos micropoluentes glifosato e paraquate, tanto com os padrões analíticos como com as suas formulações comerciais TROP® e TOCHA®, respectivamente. Foram realizadas análises de sobrevivência celular em diferentes períodos de tempo, da geração de EROs e de dosagem das enzimas catalase (CAT) e superóxido dismutase (SOD) após 2 e 3 horas de exposição aos pesticidas. Para o ensaio de sobrevivência celular as células provenientes da fase de crescimento logarítmico foram tratadas com os padrões analíticos glifosato e paraquate nas concentrações de 50, 100 e 200 µmol L-1. A concentração que mais afetou a taxa de crescimento da levedura foi a de 200 µmol L-1, sendo esta a concentração aplicada nos demais ensaios. Nos testes de sobrevivência com as formulações comerciais TROP® (glifosato) e TOCHA® (paraquate) a taxa de crescimento celular foi drasticamente afetada. Houve elevação na produção de EROs durante a segunda e terceira hora de exposição a quase todos os compostos analisados, exceto para o padrão analítico paraquate na segunda hora. A produção de CAT na segunda hora aumentou apenas para o padrão analítico glifosato, já na terceira hora aumentou tanto para esta substância quanto para a formulação comercial TROP®. Para as demais substâncias estudadas não houve alterações significativas na produção de CAT. A enzima SOD aumentou na presença dos padrões analíticos glifosato e paraquate na segunda hora, já na terceira hora ocorreu redução significativa na atividade enzimática frente apenas ao padrão analítico paraquate. As formulações comerciais TROP® e TOCHA® não apresentaram diferença significativa na produção de SOD em relação ao controle em nenhum dos períodos analisados. Os resultados obtidos evidenciam que há necessidade de abordar não apenas os ingredientes ativos dos pesticidas, mas principalmente as suas formulações comercias, pois é assim que os pesticidas são disponibilizados no ambiente. 2019-08-07T14:22:16Z 2019-08-07T14:22:16Z 2019-04-26 masterThesis IANOSKI, Aline Bisinella. Efeito estressor do glifosato e paraquate em Saccharomyces cerevisiae. 2019. 77 f. Dissertação (Mestrado em Ciência e Tecnologia Ambiental) - Universidade Tecnológica Federal do Paraná, Curitiba, 2019. http://repositorio.utfpr.edu.br/jspui/handle/1/4315 por openAccess application/pdf Universidade Tecnológica Federal do Paraná Curitiba Brasil Programa de Pós-Graduação em Ciência e Tecnologia Ambiental UTFPR |
| institution |
Universidade Tecnológica Federal do Paraná |
| collection |
RIUT |
| language |
Português |
| topic |
Leveduras Herbicidas Radicais livres (Química) Saccharomyces cerevisiae Antioxidantes Enzimas Catalase Estresse oxidativo Tecnologia ambiental Yeast Herbicides Free radicals (Chemistry) Saccharomyces cerevisiae Antioxidants Enzymes Catalase Oxidative stress Green technology CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA::SANEAMENTO AMBIENTAL::QUALIDADE DO AR, DAS AGUAS E DO SOLO Ciências Ambientais |
| spellingShingle |
Leveduras Herbicidas Radicais livres (Química) Saccharomyces cerevisiae Antioxidantes Enzimas Catalase Estresse oxidativo Tecnologia ambiental Yeast Herbicides Free radicals (Chemistry) Saccharomyces cerevisiae Antioxidants Enzymes Catalase Oxidative stress Green technology CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA::SANEAMENTO AMBIENTAL::QUALIDADE DO AR, DAS AGUAS E DO SOLO Ciências Ambientais Ianoski, Aline Bisinella Efeito estressor do glifosato e paraquate em Saccharomyces cerevisiae |
| description |
Glyphosate and paraquat are herbicides used in many countries. The disposal of these products and their metabolites lead to the contamination of several environmental compartments, and are often conducted to sewage and water treatment plants. Studies report that exposure to these compounds may lead to oxidative stress in organisms. An exaggerated stimulus in the production of free radicals, such as reactive oxygen species (ROS), may be related to more than fifty diseases. One way to monitor the effect of these micropollutants on living things is to employ Saccharomyces cerevisiae as a eukaryotic model. In this context, this yeast was used to evaluate the stressor effect generated by the micropollutants glyphosate and paraquat, both with the analytical standards and with its commercial formulations TROP® and TOCHA®, respectively. Cell survival analyzes were performed at different time periods, from the generation of ROS and the determination of the catalase (CAT) and superoxide dismutase (SOD) enzymes after 2 and 3 hours of exposure to the pesticides. For the cell survival assay the cells from the logarithmic growth phase were treated with the analytical standards glyphosate and paraquat at the concentrations of 50, 100 and 200 μmol L-¹. The concentration that most affected the growth rate of the yeast was 200 μmol L-¹, and this was the concentration applied in the other assays. In the survival trials with the commercial formulations TROP® (glyphosate) and TOCHA® (paraquat) the cell growth rate was drastically affected. There was an increase in the production of ROS during the second and third hour of exposure to almost all the compounds analyzed, except for the paraquat analytical standard in the second hour. CAT production in the second hour increased only to the glyphosate analytical standard, and in the third hour it increased for both the substance and the TROP® commercial formulation. For the other substances studied, there were no significant changes in CAT production. The enzyme SOD increased in the presence of the analytical standards glyphosate and paraquat in the second hour, and in the third hour there was a significant reduction in the enzymatic activity against analytical paraquate. Commercial formulations TROP® and TOCHA® showed no significant difference in the prodution of SOD in relation to the control in any of the analyzed periods. The results obtained show that there is a need to address not only the active ingredients of pesticides, but especially their commercial formulations, as this is how pesticides are made available in the environment. |
| format |
Dissertação |
| author |
Ianoski, Aline Bisinella |
| author_sort |
Ianoski, Aline Bisinella |
| title |
Efeito estressor do glifosato e paraquate em Saccharomyces cerevisiae |
| title_short |
Efeito estressor do glifosato e paraquate em Saccharomyces cerevisiae |
| title_full |
Efeito estressor do glifosato e paraquate em Saccharomyces cerevisiae |
| title_fullStr |
Efeito estressor do glifosato e paraquate em Saccharomyces cerevisiae |
| title_full_unstemmed |
Efeito estressor do glifosato e paraquate em Saccharomyces cerevisiae |
| title_sort |
efeito estressor do glifosato e paraquate em saccharomyces cerevisiae |
| publisher |
Universidade Tecnológica Federal do Paraná |
| publishDate |
2019 |
| citation |
IANOSKI, Aline Bisinella. Efeito estressor do glifosato e paraquate em Saccharomyces cerevisiae. 2019. 77 f. Dissertação (Mestrado em Ciência e Tecnologia Ambiental) - Universidade Tecnológica Federal do Paraná, Curitiba, 2019. |
| url |
http://repositorio.utfpr.edu.br/jspui/handle/1/4315 |
| _version_ |
1805313028545052672 |
| score |
10,814766 |