Estudo dos compostos ativos da Lycium barbarum

The study of bioactive compounds in fruits has increased attracting attention due to its nutraceutical properties. The Goji berry, Lycium barbarum is an original berry cultivated mainly in the Asia region and has been studied due to its high biological activities due to its functional compounds. Thi...

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Autor principal: Appoloni, Monise Cestari
Formato: Trabalho de Conclusão de Curso (Graduação)
Idioma: Português
Publicado em: Universidade Tecnológica Federal do Paraná 2020
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Acesso em linha: http://repositorio.utfpr.edu.br/jspui/handle/1/6630
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Resumo: The study of bioactive compounds in fruits has increased attracting attention due to its nutraceutical properties. The Goji berry, Lycium barbarum is an original berry cultivated mainly in the Asia region and has been studied due to its high biological activities due to its functional compounds. This research aimed to study the total phenolic compounds, flavonoids and anthocyanins, as well as its antioxidant potential and identification and quantification of phenolic compounds present in the fruit. Different concentrations (v / v) ethanol (20%, 40%, 60%, 80%) was used as extractor solvent of phenolic compounds, which were measured by the Folin-Ciocalteu method. Total Flavonoids were estimated using the colorimetric method with aluminum chloride and the pH differential was used to determine the monomeric anthocyanins. The antioxidant potential was assessed by means of scavening methods of Radical DPPH and ABTS. The High Performance Liquid Chromatography (HPLC) was used to identify and quantify the individual phenolic compounds present in the berry. The higher ethanol content was increased with the presence of phenolic compounds, total monomeric anthocyanins and flavonoids. However, the samples showed high antioxidant power to extraction with a lower alcohol concentration. By means of HPLC were identified as catechin, rutin, chlorogenic acid and, finally, pcoumaric acid. Finally, its noted the importance of this study due to the lack of studies on the fruits of Lycium barbarum, since it is rich in bioactive compounds, and the extrinsic and intrinsic factors that influence the composition of these compounds in the berry.