Registro fonte: Estudo dos compostos ativos da Lycium barbarum

Estudo dos compostos ativos da Lycium barbarum

The study of bioactive compounds in fruits has increased attracting attention due to its nutraceutical properties. The Goji berry, Lycium barbarum is an original berry cultivated mainly in the Asia region and has been studied due to its high biological activities due to its functional compounds. Thi...

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Autor principal:	Appoloni, Monise Cestari
Formato:	Trabalho de Conclusão de Curso (Graduação)
Idioma:	Português
Publicado em:	Universidade Tecnológica Federal do Paraná 2020
Assuntos:	Compostos bioativos Antioxidantes Cromatografia a líquido de alta eficiência Frutas - Composição Bioactive compounds Antioxidants High performance liquid chromatography Fruit - Composition CNPQ::CIENCIAS AGRARIAS
Acesso em linha:	http://repositorio.utfpr.edu.br/jspui/handle/1/6630
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id	riut-1-6630
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spelling	riut-1-66302020-11-10T12:23:58Z Estudo dos compostos ativos da Lycium barbarum Appoloni, Monise Cestari Haminiuk, Charles Windson Isidoro Demczuk Junior, Bogdan Haminiuk, Charles Windson Isidoro Gozzo, Angela Maria Março, Paulo Henrique Compostos bioativos Antioxidantes Cromatografia a líquido de alta eficiência Frutas - Composição Bioactive compounds Antioxidants High performance liquid chromatography Fruit - Composition CNPQ::CIENCIAS AGRARIAS The study of bioactive compounds in fruits has increased attracting attention due to its nutraceutical properties. The Goji berry, Lycium barbarum is an original berry cultivated mainly in the Asia region and has been studied due to its high biological activities due to its functional compounds. This research aimed to study the total phenolic compounds, flavonoids and anthocyanins, as well as its antioxidant potential and identification and quantification of phenolic compounds present in the fruit. Different concentrations (v / v) ethanol (20%, 40%, 60%, 80%) was used as extractor solvent of phenolic compounds, which were measured by the Folin-Ciocalteu method. Total Flavonoids were estimated using the colorimetric method with aluminum chloride and the pH differential was used to determine the monomeric anthocyanins. The antioxidant potential was assessed by means of scavening methods of Radical DPPH and ABTS. The High Performance Liquid Chromatography (HPLC) was used to identify and quantify the individual phenolic compounds present in the berry. The higher ethanol content was increased with the presence of phenolic compounds, total monomeric anthocyanins and flavonoids. However, the samples showed high antioxidant power to extraction with a lower alcohol concentration. By means of HPLC were identified as catechin, rutin, chlorogenic acid and, finally, pcoumaric acid. Finally, its noted the importance of this study due to the lack of studies on the fruits of Lycium barbarum, since it is rich in bioactive compounds, and the extrinsic and intrinsic factors that influence the composition of these compounds in the berry. Fundação Araucária O estudo de compostos bioativos de frutas tem atraído cada vez mais atenção devido a suas propriedades nutracêuticas. A Goji berry, Lycium barbarum é uma baga originária da região da Ásia e vem sendo estudada em virtude de suas altas atividades biológicas devido aos seus compostos funcionais. Esta pesquisa teve por objetivo estudar os compostos fenólicos totais, flavonoides e antocianinas, assim como seu potencial antioxidante e identificação e quantificação dos compostos fenólicos presentes na fruta. Diferentes concentrações (v/v) de etanol (20%, 40%, 60%, 80%) foram utilizadas como solvente extrator dos compostos fenólicos, os quais foram quantificados por meio do método de Folin-Ciocalteau. Flavonoides totais foram estimados usando o método colorimétrico com cloreto de alumínio e o diferencial de pH foi utilizado para determinar as antocianinas monoméricas. O potencial antioxidante foi avaliado por meio dos métodos de Sequestro do Radical do DPPH e do ABTS. A Cromatografia Líquida de Alta Eficiência (CLAE) foi utilizada para identificar e quantificar os compostos fenólicos individuais presentes na baga. Quanto maior o teor de etanol, maior foi à presença dos compostos fenólicos totais, flavonoides totais e antocianinas monoméricas. No entanto, as amostras apresentaram elevado poder antioxidante para a extração com menor concentração alcoólica. Por meio da CLAE foram identificados: catequina, rutina, ácido clorogênico e por fim, ácido p-cumárico. Por fim, notou-se a importância desse estudo em virtude da escassez de estudos sobre os frutos da Lycium barbarum, já que esta é rica em compostos bioativos, e pelos fatores extrínsecos e intrínsecos que influenciam na composição destes compostos na baga. 2020-11-10T12:23:58Z 2020-11-10T12:23:58Z 2015-02-13 bachelorThesis APPOLONI, Monise Cestari. Estudo dos compostos bioativos da Lycium barbarum. 2015. 51 f. Trabalho de Conclusão de Curso (Graduação) – Universidade Tecnológica Federal do Paraná, Campo Mourão, 2015. http://repositorio.utfpr.edu.br/jspui/handle/1/6630 por openAccess application/pdf Universidade Tecnológica Federal do Paraná Campo Mourao Brasil Departamento Acadêmico de Alimentos Engenharia de Alimentos UTFPR
institution	Universidade Tecnológica Federal do Paraná
collection	RIUT
language	Português
topic	Compostos bioativos Antioxidantes Cromatografia a líquido de alta eficiência Frutas - Composição Bioactive compounds Antioxidants High performance liquid chromatography Fruit - Composition CNPQ::CIENCIAS AGRARIAS
spellingShingle	Compostos bioativos Antioxidantes Cromatografia a líquido de alta eficiência Frutas - Composição Bioactive compounds Antioxidants High performance liquid chromatography Fruit - Composition CNPQ::CIENCIAS AGRARIAS Appoloni, Monise Cestari Estudo dos compostos ativos da Lycium barbarum
description	The study of bioactive compounds in fruits has increased attracting attention due to its nutraceutical properties. The Goji berry, Lycium barbarum is an original berry cultivated mainly in the Asia region and has been studied due to its high biological activities due to its functional compounds. This research aimed to study the total phenolic compounds, flavonoids and anthocyanins, as well as its antioxidant potential and identification and quantification of phenolic compounds present in the fruit. Different concentrations (v / v) ethanol (20%, 40%, 60%, 80%) was used as extractor solvent of phenolic compounds, which were measured by the Folin-Ciocalteu method. Total Flavonoids were estimated using the colorimetric method with aluminum chloride and the pH differential was used to determine the monomeric anthocyanins. The antioxidant potential was assessed by means of scavening methods of Radical DPPH and ABTS. The High Performance Liquid Chromatography (HPLC) was used to identify and quantify the individual phenolic compounds present in the berry. The higher ethanol content was increased with the presence of phenolic compounds, total monomeric anthocyanins and flavonoids. However, the samples showed high antioxidant power to extraction with a lower alcohol concentration. By means of HPLC were identified as catechin, rutin, chlorogenic acid and, finally, pcoumaric acid. Finally, its noted the importance of this study due to the lack of studies on the fruits of Lycium barbarum, since it is rich in bioactive compounds, and the extrinsic and intrinsic factors that influence the composition of these compounds in the berry.
format	Trabalho de Conclusão de Curso (Graduação)
author	Appoloni, Monise Cestari
author_sort	Appoloni, Monise Cestari
title	Estudo dos compostos ativos da Lycium barbarum
title_short	Estudo dos compostos ativos da Lycium barbarum
title_full	Estudo dos compostos ativos da Lycium barbarum
title_fullStr	Estudo dos compostos ativos da Lycium barbarum
title_full_unstemmed	Estudo dos compostos ativos da Lycium barbarum
title_sort	estudo dos compostos ativos da lycium barbarum
publisher	Universidade Tecnológica Federal do Paraná
publishDate	2020
citation	APPOLONI, Monise Cestari. Estudo dos compostos bioativos da Lycium barbarum. 2015. 51 f. Trabalho de Conclusão de Curso (Graduação) – Universidade Tecnológica Federal do Paraná, Campo Mourão, 2015.
url	http://repositorio.utfpr.edu.br/jspui/handle/1/6630
_version_	1805317211159527424
score	10,814766

Estudo dos compostos ativos da Lycium barbarum

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