Utilização de solventes eutéticos naturais profundos na extração de betalaínas de cascas de Beta vulgaris L. var. esculenta para aplicação na indústria de alimentos
Natural deep eutectic solvents (NADES) are an alternative to conventional organic solvents used in the extraction of bioactive substances of plant origin.They are formed by primary metabolites, sugars, amino acids and organic acids, therefore, they have low toxicity and greater biodegradability. Bet...
| Autor principal: | Oliveira, Anna Karolina Gomes |
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| Formato: | Trabalho de Conclusão de Curso (Graduação) |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná
2023
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.utfpr.edu.br/jspui/handle/1/30838 |
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| Resumo: |
Natural deep eutectic solvents (NADES) are an alternative to conventional organic solvents used in the extraction of bioactive substances of plant origin.They are formed by primary metabolites, sugars, amino acids and organic acids, therefore, they have low toxicity and greater biodegradability. Betalain is a substance that gives a yellow-orange or red-violet color and is divided into betacyanins and betaxanthins. This pigment has antioxidant activity, being a compound of interest for the food and pharmaceutical industry. Beetroot is the main plant source of betalains and, taking into account the use of residues, the objective of this study was to optimize the extraction of betalains from beetroot peel (Beta vulgaris L. var esculenta) using natural deep eutectic solvents. An experimental design was carried out using the response surface methodology, using the citric acid/sucrose solvent with 30% water (m/m) in three different mass proportions (1:1, 1:2, 1:3), temperature (25, 40 and 55 °C) and time (60, 120, 180 min), where the response variable was the quantification of betalains. Then the test with the highest yield was characterized according to the amount of total phenolics and reducing and scavenging power of DPPH free radicals. As a result, the test with temperature conditions at 55 °C, time of 60 minutes and eutectic solvent citric acid/sucrose (1:1) and 30% water (w/w) showed the highest yield of 114.24 mg.100 mL-1 for betacyanins and 46.4713 mg.100 mL-1 for betaxanthins. The analyzed sample showed a total amount of phenolics of 76.4970 μg of GAE.mg-1 or 367.19 μg of GAE.mL-1 and a high percentage of inhibition of 59.78% at a concentration of 4000.84 μg.mL-1. Furthermore, the EC50 value found was 5.83 μg.mL-1. Therefore, beetroot peel proved to be a potential source of betalains. The eutectic solvent tested showed a good performance in the extraction of betalains compared to conventional solvents used. |
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