Conservation of sausages utilizing chitosan biofilm
Use of biofilms in food enable increase of shelf life and ensure the conservation thereof. Chitosan has been used on obtainment of biofilms due its excellent properties of antimicrobial, antifungal, permeable and resistant film formation. The aim of this study was to evaluate the application effect...
| Principais autores: | Engel, Gelcimara, Pizato, Solange, Kalschne, Daneysa Lahis, da Silva-Buzanello, Rosana Aparecida, Habu, Sascha, Canan, Cristiane |
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| Formato: | Artigo |
| Idioma: | Português |
| Publicado em: |
Universidade Tecnológica Federal do Paraná (UTFPR)
2016
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| Acesso em linha: |
http://periodicos.utfpr.edu.br/rebrapa/article/view/3471 |
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peri-article-34712016-12-03T13:35:03Z Conservation of sausages utilizing chitosan biofilm Engel, Gelcimara Pizato, Solange Kalschne, Daneysa Lahis da Silva-Buzanello, Rosana Aparecida Habu, Sascha Canan, Cristiane Coating; Antifungal property; Shelf life; Meat product. Use of biofilms in food enable increase of shelf life and ensure the conservation thereof. Chitosan has been used on obtainment of biofilms due its excellent properties of antimicrobial, antifungal, permeable and resistant film formation. The aim of this study was to evaluate the application effect of chitosan biofilms on conservation of sausages vacuum-packed or bulk packaging, stored at 10 °C for 10 days. For biofilm obtaining, chitosan (85% of deacetylation) was dissolved in acetic acid (0.5 mol L-1), at concentrations of 1% (T1) and 2% (T2). Sausages produced in industrial line were coating with T1 and T2 to immersion, stored at vacuum-packed or bulk packaging, and compared to a control (T3, without coating). Microbiologic analyses to determination of total and thermotolerant coliforms, mesophilic aerobic count, coagulase-positive Staphylococcus, and molds and yeasts were carried out during the storage at 10 °C, at times of 0, 14, 21 and 28 days, in order to study the antimicrobial and antifungal behavior of biofilm on the sausage. The biofilm showed no inhibitory action for mesophilic aerobic in all samples during the storage period. In molds and yeasts analyses were no significant difference between the samples T1, T2 and T3, for the vacuum packed sausages, indicating that the biofilm chitosan showed antifungal properties in the studied conditions. Further, in yeast count was observed that T2 was more effective inhibition than T1. In all experimental conditions, the coagulase-positive Staphylococcus count, and determination of total and thermotolerant coliforms showed values lower than 2 CFU g-1 and 0.3 MPN g-1, respectively. Application of chitosan biofilms in sausages can result in antifungal effect when associated to vacuum packing. Universidade Tecnológica Federal do Paraná (UTFPR) 2016-08-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://periodicos.utfpr.edu.br/rebrapa/article/view/3471 10.3895/rebrapa.v7n1.3471 Brazilian Journal of Food Research; v. 7, n. 1 (2016); 42 - 57 Brazilian Journal of Food Research; v. 7, n. 1 (2016); 42 - 57 2448-3184 10.3895/rebrapa.v7n1 por http://periodicos.utfpr.edu.br/rebrapa/article/view/3471/pdf_1 http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidade Tecnológica Federal do Paraná |
| collection |
PERI |
| language |
Português |
| format |
Artigo |
| author |
Engel, Gelcimara Pizato, Solange Kalschne, Daneysa Lahis da Silva-Buzanello, Rosana Aparecida Habu, Sascha Canan, Cristiane |
| spellingShingle |
Engel, Gelcimara Pizato, Solange Kalschne, Daneysa Lahis da Silva-Buzanello, Rosana Aparecida Habu, Sascha Canan, Cristiane Conservation of sausages utilizing chitosan biofilm |
| author_sort |
Engel, Gelcimara |
| title |
Conservation of sausages utilizing chitosan biofilm |
| title_short |
Conservation of sausages utilizing chitosan biofilm |
| title_full |
Conservation of sausages utilizing chitosan biofilm |
| title_fullStr |
Conservation of sausages utilizing chitosan biofilm |
| title_full_unstemmed |
Conservation of sausages utilizing chitosan biofilm |
| title_sort |
conservation of sausages utilizing chitosan biofilm |
| description |
Use of biofilms in food enable increase of shelf life and ensure the conservation thereof. Chitosan has been used on obtainment of biofilms due its excellent properties of antimicrobial, antifungal, permeable and resistant film formation. The aim of this study was to evaluate the application effect of chitosan biofilms on conservation of sausages vacuum-packed or bulk packaging, stored at 10 °C for 10 days. For biofilm obtaining, chitosan (85% of deacetylation) was dissolved in acetic acid (0.5 mol L-1), at concentrations of 1% (T1) and 2% (T2). Sausages produced in industrial line were coating with T1 and T2 to immersion, stored at vacuum-packed or bulk packaging, and compared to a control (T3, without coating). Microbiologic analyses to determination of total and thermotolerant coliforms, mesophilic aerobic count, coagulase-positive Staphylococcus, and molds and yeasts were carried out during the storage at 10 °C, at times of 0, 14, 21 and 28 days, in order to study the antimicrobial and antifungal behavior of biofilm on the sausage. The biofilm showed no inhibitory action for mesophilic aerobic in all samples during the storage period. In molds and yeasts analyses were no significant difference between the samples T1, T2 and T3, for the vacuum packed sausages, indicating that the biofilm chitosan showed antifungal properties in the studied conditions. Further, in yeast count was observed that T2 was more effective inhibition than T1. In all experimental conditions, the coagulase-positive Staphylococcus count, and determination of total and thermotolerant coliforms showed values lower than 2 CFU g-1 and 0.3 MPN g-1, respectively. Application of chitosan biofilms in sausages can result in antifungal effect when associated to vacuum packing. |
| publisher |
Universidade Tecnológica Federal do Paraná (UTFPR) |
| publishDate |
2016 |
| url |
http://periodicos.utfpr.edu.br/rebrapa/article/view/3471 |
| _version_ |
1805294261664481280 |
| score |
10,814766 |